A genetical screening of a protein trap library for active chromatin-binding factors on polytene chromosome in Drosophila
| Project/Area Number |
22770166
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Research Collaborator |
LIM Jinseon 東京大学, 分子細胞生物学研究所, 博士課程
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 転写 / クロマチン / 核内受容体 / エクダイソン / エクダイソンレセプター / 転写制御 |
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| Research Abstract |
It is evident that gene regulation is coupled with chromatin reorganaization, but factors and molecular basis involving in this process remain to be identified. In the present study, factors responsible for ecdysone-induced puff formation were genetically screened in Drosophila fly by establishing an EGFP protein-trap approach. Among the candidates, Drosophila CG11138/Ringfect was found as a co-activator for ecdysone receptor (EcR). Through biochemical characterization, Ringfect could be shown to serve as a histone chaperon. We describe the identification of novel puff-localized factors by screening the protein trap transgenic fly library. Drosophila CG11138/Ringfect and enolase were genetically identified as a puff-localized factor and a co-activator, and interacted with EcR in S2 cell. Thus, the present study suggests that Ringfect and enolase are categorized as a novel class of nuclear receptor co-activators.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Epigenetic silencing of core histone genes by HERS in drosophila2012
Author(s)
Ito S, Fujiyama-Nakamura S, Kimura S, Lim J, Kamoshida Y, Shiozaki-Sato Y, Sawatsubashi S, Suzuki E, Tanabe M, Ueda T, Murata T, Kato H, Ohtake F, Fujiki R, Miki T, Kouzmenko A, Takeyama K, Kato S
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Journal Title
Molecular Cell
Volume: Vol.45 (4)
Pages: 494-504
Related Report
Peer Reviewed
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[Journal Article] CDP/cut is an osteoblastic coactivator of the vitamin D receptor (VDR)2010
Author(s)
Ochiai E, Kitagawa H, Takada I, Fujiyama S, Sawatsubashi S, Kim MS, Mezaki Y, Tsushima Y, Takagi K, Azuma Y, Takeyama K, Yamaoka K, Kato S, Kamimura T
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Journal Title
J Bone Miner. Res.
Volume: Vol.25 (5)
Pages: 1157-1166
Related Report
Peer Reviewed
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