Regulation of DNA replication fork by protein ubiquitination
| Project/Area Number |
22770170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
MIMURA Satoru 大阪大学, 大学院・理学研究科, 助教 (60432233)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | DNA複製 / ユビキチン修飾 / ゲノム安定性 / 出芽酵母 / ユビキチンリガーゼ |
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| Research Abstract |
In order to clarify the molecular function of two ubiquitin ligases, which localize at and regulate replication forks, associated proteins were screened by yeast two-hybrid screening. However, I could not obtain the evidence that these proteins are destabilized by the ubiquitin ligases in vivo.
Replication factor Ctf4 of budding yeast binds to both ubiquitin ligases. Through the over-expression analysis, nobel functional domain of Ctf4 was identified.
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Report
(3 results)
Research Products
(11 results)
