Regulation of DNA replication fork by protein ubiquitination

• Project/Area Number: 22770170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: MIMURA Satoru 大阪大学, 大学院・理学研究科, 助教 (60432233)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA複製 / ユビキチン修飾 / ゲノム安定性 / 出芽酵母 / ユビキチンリガーゼ

Research Abstract

In order to clarify the molecular function of two ubiquitin ligases, which localize at and regulate replication forks, associated proteins were screened by yeast two-hybrid screening. However, I could not obtain the evidence that these proteins are destabilized by the ubiquitin ligases in vivo.
Replication factor Ctf4 of budding yeast binds to both ubiquitin ligases. Through the over-expression analysis, nobel functional domain of Ctf4 was identified.

Research Products

• [Journal Article] Non-SCF-type F-box protein Roy1/ Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function (2011)
  • Author(s): Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T
  • Journal Title: Molecular Biology of the Cell Volume: 22 Pages: 1575-1584
  • Peer Reviewed
• [Journal Article] Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function (2011)
  • Author(s): Liu Y., Nakatsukasa K., Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., Kamura T.
  • Journal Title: Molecular Biology of the Cell Volume: 22 Pages: 1575-1584
  • Peer Reviewed
• [Presentation] Possible role of Xenopus Mcm3 and 6 isoforms during development (2011)
  • Author(s): 三村覚、橋本吉民、滝澤温彦
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-16
• [Presentation] Possible role of Xenopus Mcm3 and 6 isoforms during development (2011)
  • Author(s): 三村覚、橋本吉民、滝澤温彦
  • Organizer: 第34回日本分子生物学会年会ワークショップ(座長)
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-16
• [Presentation] Analysis of cell growth defect by Ctf4 over expression in budding yeast (2011)
  • Author(s): 中島美恵、滝澤温彦、三村覚
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-15
• [Presentation] Analysis of ceU growth defect by Ctf4 over expression in budding yeast (2011)
  • Author(s): 中島美恵、滝澤温彦、三村覚
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)(一般口頭発表)
  • Year and Date: 2011-12-15
• [Presentation] Functional Proteomics using an improved auxin-inducible degron(AID) system (2011)
  • Author(s): 小畑有以、西村浩平、三村覚、滝澤温彦、鐘巻将人
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-13
• [Presentation] Functional Proteomics using an improved auxin-inducible degron (AID) system (2011)
  • Author(s): 小畑有以、西村浩平、三村覚、滝澤温彦、鐘巻将人
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)(一般口頭発表)
  • Year and Date: 2011-12-13
• [Presentation] 複製異常とレトロトランスポジション活性化 (2011)
  • Author(s): 三村覚
  • Organizer: 遺伝研研究集会
  • Place of Presentation: 国立遺伝学研究所(静岡)
  • Year and Date: 2011-10-15
• [Presentation] 複製異常とレトロトランスポジション活性化 (2011)
  • Author(s): 三村覚
  • Organizer: 遺伝研研究集会
  • Place of Presentation: 国立遺伝学研究所(静岡)(招待講演)
  • Year and Date: 2011-10-15
• [Presentation] UBIQUITIN LIGASES INVOLVED IN THE REGULATION OF DNA REPLICATION IN BUDDING YEAST (2010)
  • Author(s): Satoru Mimura and Takumi Kamura
  • Organizer: The 7^<th> 3R meeting
  • Place of Presentation: アナクラウンホテル富山
  • Year and Date: 2010-10-29