Analysis of replication initiation systems regulated through the proteins-interacting domain of DnaA
| Project/Area Number |
22770173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA Tsutomu 九州大学, 大学院・薬学研究院, 教授 (70264059)
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| Research Collaborator |
HIGASHI Masahiro 九州大学, 大学院・薬学研究院, 大学院生
HARADA Yuji 九州大学, 大学院・薬学研究院, 大学院生
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | DNA複製制御 / 蛋白質間相互作用 / 染色体DNA複製 / 複製開始制御 / 大腸菌 |
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| Research Abstract |
The initiation of chromosomal replication is strictly controlled to ensure that chromosomal DNA is replicated only once per cell cycle in all cellular organisms. In Escherichia coli, the DnaA initiator is inactivated to repress extra initiations by the Hda-replicase clamp complex. Here, this study showed that specific amino acid residues of DnaA Nand C-terminal domains are important for interaction with Hda. Moreover, candidates of novel initiation regulation factors were searched by protein purification methods, and then identified by mass spectrometry analyses.
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Report
(3 results)
Research Products
(16 results)
