The molecular mechanism of the endogenous siRNA biogenesis

• Project/Area Number: 22770174
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Keio University
• Principal Investigator: MIYOSHI Keita 慶應義塾大学, 医学部, 助教 (20423395)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: RNAサイレンシング / RNA干渉 / 細胞内輸送 / RNAi / Dicer / Argonaute / RNA-タンパク質複合体 / 発現制御 / Aronaute

Research Abstract

Small RNAs provide specificity to protein effector complexes that repress mRNA transcription or translation, or catalyze mRNA destruction. In this research program, we tried to gain a better under standing of the molecular mechanisms underlying the endogenous siRNA(esiRNA) biogenesis. Using Drosophila cell, we found a novel cytoplasmic granules which contains the esiRNA biogenesis proteins and that this granule is necessary for the specific association between esiRNA and the effector complex.

Research Products

• [Journal Article] Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila (2011)
  • Author(s): Cernilogar, F. M., Onorati, M. C., Kothe, G. O., Burroughs, A. M., Parsi, K. M., Breiling, A., Sardo, F. L., Saxena, A., Miyoshi, K., Siomi, H., Siomi, M. C., Carninci, P., Gilmour D. S., Corona D. F. V., and Orlando, V.
  • Journal Title: Nature Volume: 480 Pages: 391-395
  • Peer Reviewed
• [Journal Article] Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and micro RNA biogenesis pathways in Drosophila (2010)
  • Author(s): Miyoshi, K., Miyoshi, T., Hartig, J. V., Siomi, H., Siomi, M. C.
  • Journal Title: RNA Volume: 16 Pages: 506-515
  • Peer Reviewed
• [Journal Article] Many ways to generate microRNA-like small RNAs : non-canonical pathways for microRNA production (2010)
  • Author(s): Miyoshi, K., Miyoshi, T., Siomi, H.
  • Journal Title: Molecular Genetics and Genomics Volume: 284 Pages: 95-103
  • Peer Reviewed
• [Presentation] R2D2 plays a role in endogenous siRNA loading onto Argonaute2 in novel cytoplasmic Dicer-2 bodies in Drosophila (2011)
  • Author(s): 荻野あきよ、三好啓太、塩見美喜子、塩見春彦
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2011-12-15
• [Presentation] R2D2 plays a role in endogenous siRNA loading onto Argonaute2 in novel cytoplasmic Dicer-2 bodies in Drosophila (2011)
  • Author(s): 荻野あきよ
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-15
• [Presentation] A role of novel cytoplasmic R2D2-body in esiRNA biogenesis in Drosophila (2011)
  • Author(s): Miyoshi, K., Ogino, A., Siomi, M. C., Siomi, H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2011-12-14
• [Presentation] A role of novel cytoplasmic R2D2-body in esiRNA biogenesis in Drosophila (2011)
  • Author(s): Keita Miyoshi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-14
• [Presentation] Roles of Dicer2 partners, R2D2 and Loqs-PD, in the esiRNA biogenesis pathway in Drosophila (2011)
  • Author(s): Miyoshi, K., Ogino, A., Siomi, M. C., Siomi, H.
  • Organizer: Sixteenth annual meeting of the RNA society
  • Place of Presentation: Kyoto international conference hall, JAPAN
  • Year and Date: 2011-06-17
• [Presentation] Roles of Dicer2 partners, R2D2 and Loqs-PD, in the esiRNA biogenesis pathway in Drosophila (2011)
  • Author(s): Keita Miyoshi
  • Organizer: Sixteenth annual meeting of the RNA society
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-06-17
• [Presentation] Specific requirement for Dicer2 and Loquacious-PD isoform in endogenous small-interfering RNA biogenesis in Drosophila (2011)
  • Author(s): Keita Miyoshi
  • Organizer: Keystone symposia : Mechanism and Biology of Silencing
  • Place of Presentation: Monterey, CA, USA
  • Year and Date: 2011-03-23
• [Presentation] Role of Loqs-PD in the esiRNA precursor processing in Drosophila (2011)
  • Author(s): Miyoshi, K., Ogino, A., Siomi, H., Siomi, M. C.
  • Organizer: Cell Symposia : Regulatory RNAs
  • Place of Presentation: Wyndham Hotel, Chicago, USA
• [Presentation] Specific requirement for Dicer2 and Loquacious-PD isoform in endogenous small-interfering RNA biogenesis in Drosophila. Keystone symposia (2011)
  • Author(s): Miyoshi, K., Ogino, A., Siomi, H., Siomi, M. C.
  • Organizer: Mechanism and Biology of Silencing
  • Place of Presentation: Portola Hotel & Spa, Monterey, California, USA
• [Presentation] 内在性siRNA機構におけるDicer2顆粒体の機能解析 (2010)
  • Author(s): 荻野あきよ
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] The recognition of different Dicer-Loqs complexes to small RNA precursors in Drosophila (2010)
  • Author(s): Miyoshi, K., Ogino, A., Miyoshi, T., Hartig, J. V., Siomi, H., Siomi, M. C.
  • Organizer: Exciting Biologies : Biology of Recognition
  • Place of Presentation: Sentosa Resort & Spa, SINGAPORE
  • Year and Date: 2010-10-08
• [Presentation] The recognition of different Dicer-Loqs complexes to small RNA precursors in Drosophila (2010)
  • Author(s): Keita Miyoshi
  • Organizer: Exciting Biologies : Biology of Recognition
  • Place of Presentation: SINGAPORE
  • Year and Date: 2010-10-08
• [Presentation] ショウジョウバエ内在性siRNA生合成機構の解析 (2010)
  • Author(s): 三好啓太、三好智博、荻野あきよ、塩見春彦、塩見美喜子
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 一橋記念講堂
  • Year and Date: 2010-07-27
• [Presentation] Specific requirement for Dicer2 and Loquacious-PD isoform in endogenous small-interfering RNA biogenesis in Drosophila. Keystone symposia (2010)
  • Author(s): Miyoshi, K., Miyoshi, T., Hartig, J. V., Siomi, H., Siomi, M. C.
  • Organizer: RNA Silencing : Mechanism, Biology and Application
  • Place of Presentation: Keystone Resort, USA
• [Book] Methods in Molecular Biology (2012)
  • Author(s): Miyoshi, K., Ogino A., Siomi, M. C., Siomi, H.
  • Publisher: Humana Press
• [Book] Methods in Molecular Biology (2012)
  • Author(s): Keita Miyoshi
  • Publisher: Humana Press(印刷中)
• [Book] Methods in Molecular Biology (2011)
  • Author(s): Miyoshi, K., Okada T. N., Siomi, H., Siomi, M. C.
  • Publisher: Humana Press
• [Book] Argonaute Proteins : Methods in Molecular Biology (2011)
  • Author(s): Keita Miyoshi
  • Publisher: Humana Press
• [Book] RNA technologies and their applications (2010)
  • Author(s): Saito, K., Miyoshi, K., Siomi, M. C., Siomi, H.
  • Publisher: Springer
• [Book] 内在性small interfering RNAの生合成経路と機能、実験医学増刊「拡大・進展を続けるRNA研究の最先端」 (2010)
  • Author(s): 三好啓太
  • Publisher: 羊土社
• [Book] RNA interference(RNAi)とmicro RNA(miRNA):概論、RNA工学の基礎と応用 (2010)
  • Author(s): 三好啓太、塩見美喜子
  • Publisher: シーエムシー出版
• [Book] RNA technologies and their applications (2010)
  • Author(s): Kuniaki Saito, et al.
  • Publisher: SPRINGER PG
• [Remarks]
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• [Remarks]
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• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html