| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
SgrS is a well-characterized Hfq-binding sRNA. In this project, I tried to define the SgrS region required for translation repression of ptsG mRNA, and to clarify the mechanism by which Hfq promotes the base-pairing between SgrS and ptsG mRNA. The major findings are : 1) SgrS168-181(14 nt) is a minimal base-pair region for translational repression of ptsG mRNA ; 2) the polyU tail of rho-independent terminator is essential for Hfq action ; 3) the subregion between 711 and 750 of RNase E is sufficient for the functional interaction with Hfq to support the rapid degradation of ptsG mRNA.
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