• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of multi-scale and functional imaging technique to quantify the fluctuation in intercellular signaling

Research Project

Project/Area Number 22770182
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionNational Institute of Genetics

Principal Investigator

HORIKAWA Kazuki  国立遺伝学研究所, 新分野創造センター, 准教授 (70420247)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords自己組織化 / BRET / 社会性アメーバ / 自已組織化 / cAMP
Research Abstract

To better understand the principle in self-organized pattern formation, it is important to measure fluctuating input/output-relationship of intercellular communication at the onset of pattern formation. Here, we tried to develop ultrasensitive indicators for cAMP, being the major communication signaling molecule. First, we designed the FRET-based cAMP indicator whose dynamic range and Kd was optimized for the extracellular measurement, and successfully generated ultrasensitive cAMP probe whose Kd is around 50 nM. We also developed luminescent-based BRET indicators whose Kd ranges from 50 nM to 3 microM. By utilizing these probes, we can directly measure the pattern of both intra-and intercellular cAMP concentration change which should reveal the input/output-relation during the self-organized pattern formation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] バイオイメージング細胞間シグナルの伝達パターンを捉える2011

    • Author(s)
      堀川一樹
    • Journal Title

      生物の遺伝

      Volume: 9月号 Pages: 87-91

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Spontaneous network activity visualized by ultra-sensitive Ca2+indicators, yellow Cameleon-Nano Nature Methods2010

    • Author(s)
      堀川一樹、永井健治, ほか8名
    • Volume
      7
    • Pages
      729-732
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano2010

    • Author(s)
      堀川一樹, 永井健治, ほか8名
    • Journal Title

      Nature Methods

      Volume: 7 Pages: 729-732

    • NAID

      120002834691

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Spontaneous network activity visualized by ultra-sensitive Ca2+indicators, yellow Cameleon-Nano2011

    • Author(s)
      堀川一樹
    • Organizer
      日本分子生物学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano2011

    • Author(s)
      堀川一樹
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi