Development of multi-scale and functional imaging technique to quantify the fluctuation in intercellular signaling
| Project/Area Number |
22770182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HORIKAWA Kazuki 国立遺伝学研究所, 新分野創造センター, 准教授 (70420247)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 自己組織化 / BRET / 社会性アメーバ / 自已組織化 / cAMP |
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| Research Abstract |
To better understand the principle in self-organized pattern formation, it is important to measure fluctuating input/output-relationship of intercellular communication at the onset of pattern formation. Here, we tried to develop ultrasensitive indicators for cAMP, being the major communication signaling molecule. First, we designed the FRET-based cAMP indicator whose dynamic range and Kd was optimized for the extracellular measurement, and successfully generated ultrasensitive cAMP probe whose Kd is around 50 nM. We also developed luminescent-based BRET indicators whose Kd ranges from 50 nM to 3 microM. By utilizing these probes, we can directly measure the pattern of both intra-and intercellular cAMP concentration change which should reveal the input/output-relation during the self-organized pattern formation.
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Report
(3 results)
Research Products
(5 results)
