Studies on the molecular mechanism of biogenesis and maturation of melanosomes regulated by a novel Rab32/38-binding protein

• Project/Area Number: 22770183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: OHBAYASHI Norihiko 東北大学, 大学院・生命科学研究科, 助教 (40421979)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: Rab / Varp / メラノソーム / 膜輸送 / チロシナーゼ / エフェクター / Tyrp1

Research Abstract

Rab GTPases constitute a family of small GTPases that regulate a variety of membrane trafficking events in all eukaryotic cells by recruiting their specific effector molecules. It has been reported that Rab27A, Rab32, and Rab38 are localized on the melanosomes, however, little is known about the function(s)of Rab32/38 in melanocytes. We found a novel mechanism that underlies the transport of melanogenic enzymes to melanosomes regulated by Rab32/38 and Varp that is an effector molecule of Rab32/38.

Research Products

• [Journal Article] Structure-function analysis of VP S9-ankyrin-repeat protein(Varp)in the trafficking of tyrosinase-related protein 1 in melanocytes (2011)
  • Author(s): Tamura, K., Ohbayashi, N., Ishibashi, K. & Fukuda, M
  • Journal Title: J. Boil. Chem Volume: 286 Issue: 9 Pages: 7507-7521
  • DOI: 10.1074/jbc.m110.191205
  • Peer Reviewed
• [Journal Article] Genome-wide Investigation of the Rab Binding Activity of RUN Domains: Development of a Novel Tool that Specifically Traps GTP-Rab35 (2011)
  • Author(s): Fukuda, M., Kobayashi, H., Ishibashi, K., Ohbayashi, N.
  • Journal Title: Cell Structure and Function Volume: 36 Issue: 2 Pages: 155-170
  • DOI: 10.1247/csf.11001
  • NAID: 130004053880
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] he recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking (2011)
  • Author(s): Beaumont, K. A., Hamilton, N. A., Moores, M. T., Brown, D. L., Ohbayashi, N., Cairncross, O., Cook, A. L., Smith, A. G., Misaki, R., Fukuda, M., Taguchi, T., Sturm, R. A., and Stow, J. L.
  • Journal Title: Traffic Volume: 12 Pages: 627-643
  • Peer Reviewed
• [Journal Article] The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking (2011)
  • Author(s): Beaumont, K.A., et al
  • Journal Title: Traffic Volume: 12 Issue: 5 Pages: 627-643
  • DOI: 10.1111/j.1600-0854.2011.01172.x
  • Peer Reviewed
• [Journal Article] Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2 (2010)
  • Author(s): Ohbayashi, N., Mamishi, S., Ishibashi, K., Maruta, Y., Pourakbari, B., Tamizifar, B., Mohammadpour, M., Fukuda, M., and Parvaneh, N.
  • Journal Title: Pigment Cell Melanoma Res Volume: 23 Pages: 365-374
  • Peer Reviewed
• [Journal Article] Comprehensive screening for novel Rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs (2010)
  • Author(s): Kanno, E., Ishibashi, K., Kobayashi, H., Matsui, T., Ohbayashi, N. & Fukuda, M
  • Journal Title: Traffic Volume: 11 Issue: 4 Pages: 491-507
  • DOI: 10.1111/j.1600-0854.2010.01038.x
  • Peer Reviewed
• [Journal Article] Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs. (2010)
  • Author(s): Kanno, E., Ishibashi, K., Kobayashi, H., Matsui, T., Ohbayashi, N., Fukuda, M.
  • Journal Title: Traffic Volume: 11 Pages: 491-507
  • Peer Reviewed
• [Journal Article] Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. (2010)
  • Author(s): Ohbayashi, N., Mamishi, S., Ishibashi, K., Maruta, Y., Pourakbari, B., Tamizifar, B., Mohammadpour, M., Fukuda, M., Parvaneh, N.
  • Journal Title: Pigment Cell Melanoma Res Volume: 23 Pages: 365-374
  • Peer Reviewed
• [Presentation] Varpは新規Rab32/38エフェクターとしてメラノソーム成熟に関与する (2010)
  • Author(s): 大林典彦、田村可奈子、石橋弘太郎、福田光則
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Varpは新規Rab32/38エフェクターとしてメラノソーム成熟に関与する (2010)
  • Author(s): 大林典彦、田村可南子、石橋弘太郎、福田光則
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Varp-Rab32/38複合体のメラノサイトにおける生理的意義の解明 (2010)
  • Author(s): 田村可南子、大林典彦、石橋弘太郎、福田光則
  • Organizer: 新学術領域研究「細胞内ロジスティクス」班会議
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-30
• [Presentation] Rab36はダイニン・ダイナクチン複合体依存的にリソソームの核周辺への凝集を引き起こす (2010)
  • Author(s): 松井貴英、大林典彦、丸田優人、伊藤敬、福田光則
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Presentation] Rab36はダイニン・ダイナクチン複合体依存的にリソソームの核周辺への凝集を引き起こす (2010)
  • Author(s): 松井貴英、大林典彦、丸田優人、伊藤敬、福田光則
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Remarks]
  • URL: http://www.biology.tohoku.ac.jp/lab-www/fukuda_lab/home-ja.html
• [Remarks]
  • URL: http://www.biology.tohoku.ac.jp/lab-www/fukuda_lab/home-ja.html
• [Remarks]
  • URL: http://www.biology.tohoku.ac.jp/lab-www/fukuda_lab/home-ja.html