Study of GMF-based control of actin-meshwork organization during cell migration and endocytosis

• Project/Area Number: 22770184
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: University of Tsukuba
• Principal Investigator: NAKANO Kentaro 筑波大学, 生命環境系, 講師 (50302815)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞骨格 / アクチン / エンドサイトーシス / 分裂酵母 / メンブレントラフィック / Rho / Rab / 細胞性粘菌

Research Abstract

Arp2/3 induces formation of actin meshwork which is required for membrane deformation in various type of cell movements. It has well been studied the molecular machinery activating Arp2/3 function. However, there is almost no study how Arp2/3-activity is down regulated during recycling of the actin cytoskeleton. Therefore, I decided to study the molecular function of a yeast homologue of the glia maturation factor GMF. Moreover, I tried to investigate cellular function of GmfA by using cellular mold which shows dynamic cell behavior.

Research Products

• [Journal Article] (2011) Pob1 ensures cylindrical cell shape by coupling two distinct Rho signaling events during secretory vesicle targeting (2011)
  • Author(s): Kentaro Nakano, Mika Toya, Aki Yoneda, Yukiko Asami, Akira Yamashita, Naomi Kamasaki, Masako Osumi and Masayuki Yamamoto
  • Journal Title: Traffic Volume: vol.12 Pages: 726-739
• [Journal Article] Dividing the spoils of growth and the cell cycle : The fission yeast as a model for the study of cytokinesis (2011)
  • Author(s): Anupama Goyal, Masak Takaine, Viesturs Simanis and Kentaro Nakano
  • Journal Title: Cytoskeleton Volume: vol.68 Pages: 69-88
• [Journal Article] GMF is an evolutionarily developed ADF/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton (2010)
  • Author(s): Kentaro Nakano, Hidekazu Kuwayama, Masato Kawasaki, Osamu Numata and Masak Takaine
  • Journal Title: Cytoskeleton Volume: vol.67 Pages: 373-382
• [Journal Article] GMF is an evolutionarily developed ADF/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton (2010)
  • Author(s): Kentaro Nakano, Hidekazu Kuwayama, Masato Kawasaki, Osamu Numata, Masak Takaine
  • Journal Title: Cytoskeleton Volume: 67 Pages: 373-382
  • Peer Reviewed
• [Presentation] GMF is an evolutionarily developed ADF/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton (2010)
  • Author(s): Kentaro Nakano, Hidekazu Kuwayama, Masato Kawasaki, Osamu Numata and Masak Takaine
  • Organizer: Actin,Cytoskeleton, and Nucleus
  • Place of Presentation: シンガポール国立大学、シンガポール
  • Year and Date: 2010-11-11
• [Presentation] GMF is an evolutionarily developed ADF/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton (2010)
  • Author(s): Kentaro Nakano, Hidekazu Kuwayama, Masato Kawasaki, Osamu Numata, Masak Takaine
  • Organizer: Actin, the Cytoskeleton, and the Nucleus
  • Place of Presentation: シンガポール国立大学(シンガポール)
  • Year and Date: 2010-11-09
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/organelle/nakano.html
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/organelle/nakano.html
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/organelle/nakano.html