The novel mechanism of stress recognition through the interaction of SOD1 with Derlin-1

• Project/Area Number: 22770187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: KADOWAKI Hisae 東京大学, 大学院・薬学系研究科, 特任研究員 (40568200)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: タンパク質 / 小胞体ストレス / Derlin-1 / SOD1

Research Abstract

The structure of wild type SOD1 is thought to be changed by some cellular stress, and induces the endoplasmic reticulum stress through the interaction with Derlin-1, which is the important component of endoplasmic reticulum quality control system. Then, we tried to identify the factor which induces the interaction between wild type SOD1 and Derlin-1. Then, we identified the serum starvation induces the interaction and triggers endoplasmic reticulum stress.

Research Products

• [Journal Article] CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response (2010)
  • Author(s): Maruyama, T., et al.
  • Journal Title: EMBO J. Volume: 29 Pages: 2501-2514
  • Peer Reviewed
• [Journal Article] A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants
  • Author(s): Fujisawa T, Homma K, Yamaguchi N, Kadowaki H, Tsuburaya N, Naguro I, Matsuzawa A, Takeda K, Takahashi Y, Goto J, Tsuji S, Nishitoh, Ichijo H
  • Journal Title: Ann. Neurol Volume: (印刷中)
  • Peer Reviewed
• [Presentation] A common mechanism for motoneuron death by ALS-linked SOD1mutations (2011)
  • Author(s): Fujisawa T, Homma K, Tsuburaya N, Yamaguchi N, Kadowaki H, Nishitoh H, Ichijo H.
  • Organizer: Gordon Research Conference, Stress Proteins in Growth, Development & Disease
  • Place of Presentation: Italy
• [Presentation] A common mechanism for motoneuron death by ALS-linked SOD1 mutations (2011)
  • Author(s): Fujisawa T, Homma K, Tsuburaya N, Yamaguchi N, Kadowaki H, Nishitoh H, Ichijo H.
  • Organizer: 13th International TNF Conference
  • Place of Presentation: Awajishima
• [Presentation] A common mechanism for motoneuron death by ALS-linked SOD1 mutations (2011)
  • Author(s): Fujisawa T, Homma K, Tsuburaya N, Yamaguchi N, Kadowaki H, Nishitoh H, Ichijo H
  • Organizer: Gordon Research Conference, Stress Proteins in Growth, Development & Disease
  • Place of Presentation: Lucca, Italy
• [Presentation] A common mechanism for motoneuron death by ALS-linked SOD1 mutations (2011)
  • Author(s): Fujisawa T, Homma K, Tsuburaya N, Yamaguchi N, Kadowaki H, Nishitoh H, Ichijo H
  • Organizer: 13th International TNF Conference
  • Place of Presentation: 淡路島
• [Presentation] A common mechanism for ER stress by ALS-linked SOD1 mutations (2010)
  • Author(s): Fujisawa, T., et al.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良
• [Presentation] A common mechanism for ER stress by ALS-linked SOD1 mutations (2010)
  • Author(s): 藤澤貴央, 他
  • Organizer: 東京大学医薬連携GCOEリトリート
  • Place of Presentation: 幕張
• [Presentation] ALSにおける変異型SOD1による小胞体ストレス誘導の分子メカニズム (2010)
  • Author(s): 藤澤貴央, 他
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸