Structural and functional variations of vertebrate cilia

• Project/Area Number: 22770190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: University of Yamanashi
• Principal Investigator: NARITA Keishi 山梨大学, 大学院・医学工学総合研究部, 講師 (50452131)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 繊毛 / 脈絡 / 上皮細胞 / プロテオミクス / ライブセルイメージング / 脈絡叢

Research Abstract

In the present study, we obtained the following results.(1) Proteomic analysis of unique, multiple 9+0 cilia in choroid plexus epithelial cells(CPECs) identified 868 proteins.(2) Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1-2 weeks.(3) Ultrastructural analysis revealed the presence of not only 9+0 but also 9+2 and atypical ciliary subtypes in neonatal CPEC.(4) A set of candidate genes that would regulate ciliary number per cell was identified based on DNA microarray analysis.

Research Products

• [Journal Article] Proteomic analysis ofmultiple primary cilia reveals a novelmode of ciliary development inmammals (2012)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Yuta Nonami, Hiroko Ao-Kondo, Toshimitsu Suzuki, Hideki Nakamura, Kazuhiro Yamakawa, Masaaki Oyama, Takafumi Inoue, and Sen Takeda
  • Journal Title: Biology Open Volume: (in press)
  • Peer Reviewed
• [Journal Article] 神経系細胞の一次繊毛に見られるセンサー機能 (2012)
  • Author(s): 竹田扇、成田啓之
  • Journal Title: ClinicalNeuroscience Volume: 30(4) Pages: 465-8
• [Journal Article] Structure and function of vertebratecilia, towards a new taxonomy (2012)
  • Author(s): Sen Takeda, Keishi Narita
  • Journal Title: Differentiation Volume: 83(2)
  • Peer Reviewed
• [Journal Article] 神経系細胞の一次繊毛に見られるセンサー機能 (2012)
  • Author(s): Sen Takeda, Keishi Narita
  • Journal Title: Clinical Neuroscience Volume: 30 Pages: 465-468
• [Journal Article] Unique property ofchoroid plexus epithelium primarycilia (2011)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Hiroko Ao-Kondo, Masaaki Oyama, Sen Takeda
  • Journal Title: The Journal of Physiological Sciences Volume: 61
• [Journal Article] Differential neuroprotective activityof two different grape seed extracts (2011)
  • Author(s): Keishi Narita, Masashi Hisamoto, Tohru Okuda, Sen Takeda
  • Journal Title: PLoS One Volume: 6(1)
  • URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0014575
  • Peer Reviewed
• [Journal Article] Structure and function of vertebrate cilia, towards a new taxonomy (2011)
  • Author(s): Takeda, S., Narita, K.
  • Journal Title: Differentiation Volume: 83 Issue: 2 Pages: S4-S11
  • DOI: 10.1016/j.diff.2011.11.002
  • Peer Reviewed
• [Journal Article] Differential neuroprotective activity of two different grape seed extracts. (2011)
  • Author(s): 成田啓之
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Unique property of choroid plexus epithelium primary cilia. (2011)
  • Author(s): 成田啓之
  • Journal Title: The journal of physiological sciences Volume: 61
• [Presentation] プロテオミクス解析が明かした脈絡叢上皮細胞の繊毛多様性 (2012)
  • Author(s): 成田啓之、秦裕子、野波祐太、近藤裕子、中村英樹、尾山大明、井上貴文、竹田扇
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学
  • Year and Date: 2012-03-26
• [Presentation] Proteomics of choroid piexus epithelial cilia reveals unexpected mosaic distribution of heterogeneous cilia in a cellular level (2012)
  • Author(s): 成田啓之
  • Organizer: 第117回日本解剖学会
  • Place of Presentation: 山梨大学(甲府)
  • Year and Date: 2012-03-26
• [Presentation] Structural and functional transition of choroid plexus epithelial cilia revealed by proteomic analysis (2011)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Yuta Nonami, Hiroko Ao-Kondo, Masaaki Oyama, Takafumi Inoue, Sen Takeda
  • Organizer: 第51回米国細胞生物学会
  • Place of Presentation: Coloradoconvention center, Denver, CO
  • Year and Date: 2011-12-04
• [Presentation] Structural and functional transition of choroid plexus epithelial cilia revealed by proteomic analysis (2011)
  • Author(s): 成田啓之
  • Organizer: 第51回米国細胞生物学会
  • Place of Presentation: Colorado convention center (Denver, CO)
  • Year and Date: 2011-12-04
• [Presentation] Putative function of TRPV4on the choroid plexus primary cilia (2011)
  • Author(s): Shohei Sasamoto, Keishi Narita, Sen Takeda
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Proteomic analysis ofchoroid plexus epithelial primary ciliarevealed its functional transition (2011)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Yuta Nonami, Hiroko Ao-Kondo, Masaaki Oyama, Takafumi Inoue, Sen Takeda
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Proteomic analysis of choroid piexus epithelial primary cilia revealed its functional transition (2011)
  • Author(s): 成田啓之
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-09-17
• [Presentation] Putative function of TRPV4 on the choroid piexus primary cilia (2011)
  • Author(s): 笹本祥平、成田啓之
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-09-17
• [Presentation] Functional transition of choroid plexus epithelial primary cilia revealed by proteomics analysis (2011)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Hiroko Ao-Kondo, Masaaki Oyama, Sen Takeda
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-28
• [Presentation] Functional transition of choroid plexus epithelial primary cilia revealed by proteomics analysis (2011)
  • Author(s): 成田啓之
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2011-06-28
• [Presentation] Proteomic analyses of cilia to explore its diversity and differentiation (2010)
  • Author(s): Keishi Narita, Hiroko Kozuka-Hata, Hiroko Ao-Kondo, Masaaki Oyama, Sen Takeda
  • Organizer: 第50回米国細胞生物学会
  • Place of Presentation: Pennsylvania convention center, Philadelphia, PA
  • Year and Date: 2010-12-12