Molecular dissection of sister chromatid cohesion

• Project/Area Number: 22770201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SHINTOMI Keishi 独立行政法人理化学研究所, 平野染色体ダイナミクス研究室, 研究員 (60462694)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 染色体 / 姉妹染色分体 / コヒーシン / コンデンシン / カエル卵無細胞系 / 姉妹染色分体接着

Research Abstract

Cohesin is a protein complex essential for sister chromatid cohesion. The goal of this study is to understand molecular basis behind the cohesion process. My initial approach using the cohesin complexes reconstituted from recombinant subunits fell through because of some technical reasons. Unexpectedly, I have found that balancing action between cohesin and condensin, which is required for chromosome condensation, determines shapes of metaphase chromosomes in a cell-free system from frog eggs.

Research Products

• [Journal Article] The relative ratio of condensin I to II determines chromosome shapes (2011)
  • Author(s): Keishi Shintomi and Tatsuya Hirano
  • Journal Title: Genes Dev Volume: 25 Pages: 1464-1469
  • Peer Reviewed
• [Journal Article] MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II (2011)
  • Author(s): Daisuke Yamashita, Keishi Shintomi, Takao Ono, Ioannis Gavvovidis, Detlev Schindler, Heidemarie Neitzel, Marc Trimborn, and Tatsuya Hirano
  • Journal Title: J Cell Biol Volume: 194 Pages: 841-854
  • Peer Reviewed
• [Journal Article] The relative ratio of condensin I to II determines chromosome shapes (2011)
  • Author(s): K.Shintomi
  • Journal Title: Genes & Dev. Volume: 25 Issue: 14 Pages: 1464-1469
  • DOI: 10.1101/gad.2060311
  • Peer Reviewed
• [Journal Article] MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II (2011)
  • Author(s): D.Yamashita
  • Journal Title: J.Cell Biol. Volume: 194 Issue: 6 Pages: 841-854
  • DOI: 10.1083/jcb.201106141
  • Peer Reviewed
• [Journal Article] Sister chromatid resolution : a cohesin releasing network and beyond (2010)
  • Author(s): Keishi Shintomi and Tatsuya Hirano
  • Journal Title: Chromosoma Volume: 119 Pages: 459-467
  • Peer Reviewed
• [Presentation] 精製タンパク質を使って染色体を作ることができるか (2012)
  • Author(s): 新冨圭史、平野達也
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-26
• [Presentation] 精製タンパク質を使って染色体を作ることができるか? (2012)
  • Author(s): 新冨圭史、平野達也
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-26
• [Presentation] How condensins and cohesin shape chromosomes : Lessons from frog eggs (2011)
  • Author(s): Keishi Shintomi
  • Organizer: RIKEN 2011 Joint Retreat
  • Place of Presentation: 掛川
  • Year and Date: 2011-02-01
• [Presentation] 2つのコンデンシンの存在比と作用タイミングが染色体形状を決定する (2010)
  • Author(s): 新冨圭史、平野達也
  • Organizer: 第33回日本分子生物学会年会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Remarks]
  • URL: http://www.riken.jp/chromdyna
• [Remarks]
  • URL: http://www.riken.jp/chromdyna/