Mechanisms of formation and maintenance of signaling center during brain patterning

• Project/Area Number: 22770206
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Masanori 東北大学, 大学院・医学系研究科, 助教 (20361074)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: (A)細胞分化 / 菱脳 / 境界 / ゼブラフィッシュ / イメージング / Eph-ephrin / コンパートメント / 未分化性維持 / ニューロン分析

Research Abstract

In this study, I examined the processes of rhombomere-boundary formation in the Pax6 mutant rat hindbrain. This study suggests that Pax6 may repress neuronal differentiation through Notch signaling pathway in the rhombomere-boundary cells. I also analyzed cell behavior in the hindbrain of EphA4/ephrinB3b double knockdown-zebrafish embryos using high resolution-confocal time-lapse imaging. GFP^+ interface cells at r3/4 boundary abnormally elongated along the A-P axis and then migrated into r4 region, suggesting that EphA4/ephrinB3b signaling is required for sharpening rhombomere boundaries via regulation of cell migration and cell polarity.

Research Products

• [Journal Article] Cyclin D2 in the basal process of neural progenitors is linked to non-equivalent cell fates (2012)
  • Author(s): Tsunekawa, Y. Brutto, JM, Takahashi, M. Polleux, F. Tan, SS. Osumi, N.
  • Journal Title: EMBO J Volume: Mar 6
  • Peer Reviewed
• [Journal Article] Pax6 regulates rhombomere boundary cell specification in the rat hindbrain (2011)
  • Author(s): Takahashi, M. and Osumi, N.
  • Journal Title: Mech Volume: Dev. 128 Pages: 289-302
  • Peer Reviewed
• [Journal Article] Pax6 regulates boundary-cell specification in the rat hind brain (2011)
  • Author(s): Takahashi, M., Osumi, N
  • Journal Title: Mech Dev Volume: 128(5-6) Issue: 5-6 Pages: 289-302
  • DOI: 10.1016/j.mod.2011.04.001
  • Peer Reviewed
• [Journal Article] The method of rodent whole embryo culture using the rotator-type bottle culture system (2010)
  • Author(s): Takahashi, M. and Osumi, N.
  • Journal Title: J. Vis Volume: Exp. 42 Pages: 2170-2170
  • Peer Reviewed
• [Journal Article] The method of rodent whole embryo culture using the rotator-type bottle culture system. (2010)
  • Author(s): Takahashi, M., Osumi, N.
  • Journal Title: J.Vis.Exp. Volume: 42
  • Peer Reviewed
• [Presentation] Pax6 regulates boundary cell specification in the rat hindbrain (2011)
  • Author(s): Takahashi, M. and Osumi, N.
  • Organizer: The 1st Tohoku International Symposium on Multidisciplinary Neuroscience
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-01-21
• [Presentation] Pax6 regulates boundary-cell specification in the rat hindbrain. (2011)
  • Author(s): Takahashi M., Osumi, N.
  • Organizer: The 1st Tohoku International Symposium on Multidisciplinary Neuroscience
  • Place of Presentation: Sendai
  • Year and Date: 2011-01-21
• [Presentation] Pax6 is involved in specification of rhombomere boundary cells in the rat hindbrain (2010)
  • Author(s): Takahashi, M. and Osumi, N.
  • Organizer: 3rd International Congress on Stem Cells and tissue Formation
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2010-07-13
• [Presentation] Pax6 is involved in specification of rhombomere boundary cells in the rat hindbrain. (2010)
  • Author(s): Takahashi M., Osumi, N.
  • Organizer: 3rd International Congress on Stem Cells and tissue Formation.
  • Place of Presentation: Dresden(ドイツ)
  • Year and Date: 2010-07-13