A genome-wide analysis of let-7 micron-dependent developmental timing pathway

• Project/Area Number: 22770207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: University of Tsukuba
• Principal Investigator: NIWA Ryusuke 筑波大学, 生命環境系, 助教 (60507945)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 発生・分化 / 発現制御 / 線虫 / 発生タイミング / マイクロRNA / 転写因子 / クロマチン構造制御因子 / 核内受容体

Research Abstract

The development of multicellular organisms is tightly regulated under a precise time schedule. To unravel the molecular mechanisms underlying developmental timing in multicellular organisms, I used the nematode Caenorhabditis elegans as the model organism. I identified and characterized genes involved in the let-7 microRNA pathway, which is essential for the larva-to-adult transition. I found that the let-7 microRNA pathway is controlled by several transcription regulatory factors and receptor and chromatin-remodeling factors.

Research Products

• [Journal Article] Identification of a Spatio-Temporal Enhancer Element for the Alzheimer's amyloid precursor protein-like-1 Gene in the Nematode Caenorhabditis elegans (2010)
  • Author(s): yusuke Niwa and Kazumasa Hada
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 12巻 Pages: 1497-2500
  • NAID: 10027728374
  • Peer Reviewed
• [Journal Article] Slack, and Ryusuke Niwa (2010) The nuclear receptor gene nhr-25 plays multiple roles in the C (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J
  • Journal Title: elegans heterochronic gene network to control the larva-to-adult transition. Developmental Biology Volume: 344巻 Pages: 1100-1109
  • Peer Reviewed
• [Journal Article] The nuclear receptor gene nhr-25 plays multiple roles in the Caenorhabditis elegans heterochronic gene network to control the larva-to-adult transition (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J.Slack, Ryusuke Niwa
  • Journal Title: Developmental Biology Volume: 344 Pages: 1100-1109
  • NAID: 120007137960
  • Peer Reviewed
• [Journal Article] Identification of a spatio-temporal enhancer element for the Alzheimer's amyloid precursor protein-like-1 gene in the nematode Caenorhabditis elegans (2010)
  • Author(s): Ryusuke Niwa, Kazumasa Hada
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Pages: 2497-2500
  • NAID: 10027728374
  • Peer Reviewed
• [Presentation] The histone H3 methyltransferase LIN-59 regulates the expression of the let-7 family microRNAs during the larva-to-adult transition in C elegans (2012)
  • Author(s): Kazumasa Hada, Minori Onda, Yuta Takahashi, Haruyasu Amano, Sora Enya, Keiko Hirota, Akiyoshi Fukamizu and Ryusuke Niwa
  • Organizer: 5th East Asian Worm Meeting
  • Place of Presentation: Chientan Youth Activity Center(台湾台北市)
  • Year and Date: 2012-06-29
• [Presentation] The histone methyltransferase LIN-59 controls the expression of the let-7 family microRNAs during the larva-to-adult transition in the nematode Caenorhabditis elegans (2011)
  • Author(s): Kazumasa Hada, Haruyasu Amano, Minori Onda, Sora Enya and Ryusuke Niwa
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 線虫C.elegansの発生タイミングを制御するクロマチンリモデリング因子の同定 (2011)
  • Author(s): 波田一誠、塩谷天、恩田美紀、丹羽隆介
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (東日本大震災のために学会開催および口頭発表は中止。但し、本会での発表自体は成立している旨、学会長より告知されている。)
  • Year and Date: 2011-03-26
• [Presentation] 線虫C. elegansの発生タイミングを制御するクロマチンリモデリング因子の同定 (2011)
  • Author(s): 波田一誠、塩谷天、恩田美紀、丹羽隆介
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 東日本大震災により学会大会は中止となったが、発表は成立していることが正式に認められている
• [Presentation] Slack and Ryusuke Niwa 『A role of the nuclear receptor gene nhr-25 in the C. elegans heterochronic gene network』 (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J
  • Organizer: International Symposium on Cell Functions Mediated by Small Molecules
  • Place of Presentation: 筑波大学総合研究棟D棟
  • Year and Date: 2010-11-08
• [Presentation] A role of the nuclear receptor gene nhr-25 in the C.elegans heterochronic genenetwork (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J.Slack, Ryusuke Niwa
  • Organizer: International Symposium on Cell Functions Mediated by Small Molecules
  • Place of Presentation: 筑波大学総合研究棟D棟
  • Year and Date: 2010-11-08
• [Presentation] Slack and Ryusuke Niwa『The nuclear receptor gene nhr-25 plays multiple roles in the C. elegans heterochronic gene network to control the larva-to-adult transition』 (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J
  • Organizer: East Asian Worm Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(代々木)
  • Year and Date: 2010-07-12
• [Presentation] The nuclear receptor gene nhr-25 plays multiple roles in the C.elegans heterochronic gene network to control the larva-to-adult transition (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J.Slack, Ryusuke Niwa
  • Organizer: 4th East Asian Worm Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(代々木)
  • Year and Date: 2010-07-12
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/~rniwa/
• [Remarks]
  • URL: http://www.trios.tsukuba.ac.jp/Profiles/0005/0005746/profile.html
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/~rniwa
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/~rniwa