Molecular analysis of specification along anteiroposterior axis in body of vertebrates

• Project/Area Number: 22770208
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Saitama University
• Principal Investigator: KAWAMURA Akinori 埼玉大学, 大学院・理工学研究科, 講師 (10466691)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 初期発生 / 突然変異体 / 転写制御機構 / ゼブラフィッシュ / エンハンサー / 脊椎動物 / 体節形成 / 前後軸形成 / 発生 / 軸形成 / パターニング / スクリーング / スクリーニング

Research Abstract

During embryogenesis of vertebrates, the repeated and segmental structure, somites, are formed, and subsequently specification along the anteroposterior axis is established in vertebrates, Hox genes have been known to play roles in the specification of identity along the anteroposterior axis. However, the molecular mechanisms underlying this ordered expression patterns of Hox genes remains poorly understood. In this study, we analyzed the zebrafish mutant and identified candidate genes by genetic analyses. In addition, we analyzed the transcriptional regulation of hairy-related gene, which play important roles in the somite patterning. The DNA region downstream of hairy-related gene shows enhancer activity, consistent with the endogenous gene expression. At present, we further proceed toanalyze this DNA region.

Research Products

• [Journal Article] Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages (2012)
  • Author(s): Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., Yamasu, K
  • Journal Title: Mechanisms of Development Volume: 129 Pages: 219-235
• [Journal Article] Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafishembryogenesis (2012)
  • Author(s): Khan, A., Nakamoto, A., Tai, M., Saito S., Nakayama, Y., Kawamura, A., Takeda, H., Yamasu, K
  • Journal Title: Development, Growth and Differentiation Volume: 54(7) Pages: 686-701
  • NAID: 10031136935
• [Journal Article] Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages (2012)
  • Author(s): Khan A, Nakamoto A, Okamoto S, Tai M, Nakayama Y, Kobayashi K, Kawamura A, Takeda H, Yamasu K.
  • Journal Title: Mechasmims of Development Volume: 129 Issue: 9-12 Pages: 219-235
  • DOI: 10.1016/j.mod.2012.07.007
  • Peer Reviewed
• [Journal Article] Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis. (2012)
  • Author(s): Khan A, Nakamoto A, Tai M, Saito S, Nakayama Y, Kawamura A, Takeda H, Yamasu K.
  • Journal Title: Development, Growth & Differentiation Volume: 54 Issue: 7 Pages: 686-701
  • DOI: 10.1111/j.1440-169x.2012.01369.x
  • NAID: 10031136935
  • Peer Reviewed
• [Journal Article] Ripply3, a Tbx1 repressor, is required for development of pharyngeal apparatus and its derivatives in mice (2011)
  • Author(s): Okubo, T., Kawamura, A., Takahashi, J., Yagi, H., Morishima, M., Matsuoka, R., Takada S
  • Journal Title: Development Volume: 138 Pages: 339-348
• [Journal Article] FGF receptor gene expression and its regulation by FGF signaling during early zebrafish development (2010)
  • Author(s): Ota, S., Tonou-Fujimori, N., Nakayama, Y., Ito, Y., Kawamura, A., Yamasu, K
  • Journal Title: Genesis Volume: 48 Pages: 707-716
• [Journal Article] FGF receptor gene expression and its regulation by FGF signaling during early zebrafish development. (2010)
  • Author(s): Ota S., Tonou-Fujimori N., Nakayama Y., Ito Y., Kawamura A., Yamasu K
  • Journal Title: Genesis Volume: 48 Pages: 707-716
  • Peer Reviewed
• [Presentation] Tbx1の抑制因子Ripply3はPax9の転写を抑制し咽頭弓の発生を制御する (2010)
  • Author(s): 大久保直,八木寿人,川村哲規,高橋潤,森島正恵,松岡瑠美子,高田慎治
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Remarks] 埼玉大学大学院生体制御学コースHP:
  • URL: http://seitai.saitama-u.ac.jp/index.html
• [Remarks] 生体制御学コース発生生物学研究室HP:
  • URL: http://devbiol.seitai.saitama-u.ac.jp/
• [Remarks] http://devbiol.seitai.saitama-u.ac.jp/
• [Remarks]
  • URL: http://devbiol.seitai.saitama-u.ac.jp/
• [Remarks]
  • URL: http://devbiol.seitai.saitama-u.ac.jp/