Small RNA and epigenetic control of the tudor-piwi pathway in the germline
| Project/Area Number |
22770210
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 生殖細胞 / ゲノム / RNA / 発生 / トランスポゾン / トランスポ |
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| Research Abstract |
The piwi-tudor pathway is essential for piRNA biogenesis and it acts to suppress retrotransposons in the germline. In this study, we carried out mass spec and high throughput sequencing analyses of the piwi-tudor complex and identified novel factors that function to control retrotransposons in germline stem cells at RNA and epigenetic levels. Genetic and biochemical analyses of a mutant of a mouse piwi revealed(1) an essential and physiological role of the piwi slicer activity in the germline and(2) a complementarity dependent rule required for the slicer activity mediated piRNA biogenesis. In a collaborative work, we characterized piwi pathway operation in an established cell line of spermatogonia and carried out multi-omics analyses. These results provide novel insights into key mechanisms that protect the germline genome from transposon activity.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Tudor domain containing 7(Tdrd7) isessential for dynamic ribonucleoprotein(RNP) remodeling of chromatoid bodies during spermatogenesis2011
Author(s)
Tanaka T, Hosokawa M, Vagin VV, Reuter M, Hayashi E, Mochizuki AL, Kitamura K, Yamanaka H, Kondoh G, Okawa K, Kuramochi-Miyagawa S, Nakano T, Sachidanandam R, Hannon GJ, Pillai RS, Nakatsuji N, ChumaS.
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Journal Title
Proc Natl Acad SciUSA
Volume: 108(26)
Pages: 10579-84
Related Report
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[Journal Article] MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline2011
Author(s)
Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, NoceT, Nakano T, Nakatsuji N, Lin H, Sasaki H.
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Journal Title
Dev Cell
Volume: 20(3)
Pages: 364-75
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[Journal Article] MVH in piRNA processing and gene silencing of retrotransposons2010
Author(s)
Kuramochi-Miyagawa S, Watanabe T, GotohK, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T.
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Journal Title
Genes Dev
Volume: 24(9)
Pages: 887-92
Related Report
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