| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
The piwi-tudor pathway is essential for piRNA biogenesis and it acts to suppress retrotransposons in the germline. In this study, we carried out mass spec and high throughput sequencing analyses of the piwi-tudor complex and identified novel factors that function to control retrotransposons in germline stem cells at RNA and epigenetic levels. Genetic and biochemical analyses of a mutant of a mouse piwi revealed(1) an essential and physiological role of the piwi slicer activity in the germline and(2) a complementarity dependent rule required for the slicer activity mediated piRNA biogenesis. In a collaborative work, we characterized piwi pathway operation in an established cell line of spermatogonia and carried out multi-omics analyses. These results provide novel insights into key mechanisms that protect the germline genome from transposon activity.
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