microRNA-mediated regulation of the crosstalk between muscle and vasculature

• Project/Area Number: 22770216
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Kobe University
• Principal Investigator: MISHIMA Yuichiro 神戸大学, 理学研究科, 学術研究員 (00557069)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: microRNA / ゼブラフィッシュ / 血管形成 / VEGF

Research Abstract

MicroRNAs are a novel layer of gene regulatory mechanism that induce post-transcriptional silencing. In this study, we analyzed the non-cell autonomous regulation of vasculogenesis by a muscle-specific miRNA, miR-1, in zebrafish. Our results indicated that miR-1 modulates vasculogenesis via the negative regulation of secreted factor VEGF.

Research Products

• [Journal Article] Widespread roles of microRNAs during zebrafish development and beyond (2012)
  • Author(s): Mishima Y
  • Journal Title: Development Growth and Differentiation誌 Volume: 54(1) Pages: 55-65
  • NAID: 10030404705
  • Peer Reviewed
• [Journal Article] Widespread roles of microRNAs during zebrafish development and beyond (2012)
  • Author(s): 三嶋雄一郎
  • Journal Title: Development Growth and Differentiation Volume: 54 Issue: 1 Pages: 55-65
  • DOI: 10.1111/j.1440-169x.2011.01306.x
  • NAID: 10030404705
  • Peer Reviewed
• [Journal Article] A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity (2010)
  • Author(s): Cifuentes D、Xue H、Taylor D. W.、Patnode H、Mishima Y、Cheloufi S、Ma E、Mane S、Hannon G. J.、Lawson N、Wolfe S、Giraldez AJ
  • Journal Title: Science誌 Volume: 328(5986) Pages: 1694-8
  • Peer Reviewed
• [Journal Article] A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity (2010)
  • Author(s): Daniel Cifuentes
  • Journal Title: Science Volume: 328 Pages: 1698-1698
  • Peer Reviewed
• [Presentation] Molecular mechanisms and regulations of microRNA-mediated gene silencing in zebrafish (2011)
  • Author(s): 三嶋雄一郎
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis (2011)
  • Author(s): 三嶋雄一郎
  • Organizer: The 16th Annual Meeting of the RNA Society
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2011-06-16
• [Presentation] Interactions between microRNA pathway and translation machineries (2011)
  • Author(s): 三嶋雄一郎
  • Organizer: Tokyo RNA Club the Fifth Meeting
  • Place of Presentation: 東京大学弥生講堂(東京都)
  • Year and Date: 2011-06-13
• [Presentation] Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis (2011)
  • Author(s): 三嶋雄一郎
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Portola Hotel(米国)
  • Year and Date: 2011-03-22
• [Presentation] Molecular mechanisms and regulations of microRNA-mediated gene silencing in zebrafish (2011)
  • Author(s): 三嶋雄一郎、井上邦夫
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis (2011)
  • Author(s): Mishima Y, Fukao A, Kishimoto T, Sakamoto H, Fujiwara T, Inoue K.
  • Organizer: The 16th Annual Meeting of the RNA Society
  • Place of Presentation: 京都国際会議場
• [Presentation] Interactions between microRNA pathway and translation machineries (2011)
  • Author(s): Mishima Y
  • Organizer: Tokyo RNA Club the Fifth Meeting
  • Place of Presentation: 東京大学弥生講堂
• [Presentation] Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis Keystone Symposia on Molecular and Cellular Biology "Mechanisms and Biology of RNA silencing" (2011)
  • Author(s): Mishima Y, Fukao A, Kishimoto T, Sakamoto H, Fujiwara T, Inoue K
  • Place of Presentation: Portola Plaza Hotel & Spa, Monterey, California, USA
• [Presentation] microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する (2010)
  • Author(s): 三嶋雄一郎
  • Organizer: 第33回日本分子生物学会 年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する (2010)
  • Author(s): 三嶋雄一郎
  • Organizer: 第2回RNAi研究会
  • Place of Presentation: グランドプリンスホテル広島(広島県)
  • Year and Date: 2010-08-27
• [Presentation] The Molecular Mechanisms of TNRC6 to Induce Translational Repression and Deadenylation in Zebrafish (2010)
  • Author(s): 三嶋雄一郎
  • Organizer: The 19th CDB Meeting RNA Sciences in Cell and Developmental Biology
  • Place of Presentation: 理化学研究所(兵庫県)
  • Year and Date: 2010-05-10
• [Presentation] microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する (2010)
  • Author(s): 三嶋雄一郎、岸本友良、深尾亜喜良、武田耕哲、坂本博、藤原俊伸、井上邦夫
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する (2010)
  • Author(s): 三嶋雄一郎、岸本友良、深尾亜喜良、武田耕哲、坂本博、藤原俊伸、井上邦夫
  • Organizer: 第2回RNAi研究会
  • Place of Presentation: グランドプリンスホテル広島
• [Presentation] The Molecular Mechanisms of TNRC6 to Induce Translational Repression and Deadenylation in Zebrafish (2010)
  • Author(s): Mishima Y, Fukao A, Kishimoto T, Sakamoto H, Fujiwara T, Inoue K
  • Organizer: The 19th CDB Meeting-RNA Sciences in Cell and Developmental Biology
  • Place of Presentation: 理化学研究所CDB