| Project/Area Number |
22770224
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
AMANO Takanori 国立遺伝学研究所, 系統生物研究センター, 特任研究員 (20419849)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 遺伝子発現調節 |
|---|
| Research Abstract |
Shh is localized in the posterior mesenchyme of limb buds, which is regulated by limb-specific enhancer named MFCS1.A number of mutations are known in the mammalian MFCS1, most of which cause preaxitial polydactyly. A yeast one-hybrid screen identified Msx1 as a transcription factor binding to the Hx-mutated site. In the Hxmutant, Msx1 can bind to the substituted Hx-site in MFCS1. By contrast, in other three mouse mutant, their single-base substitutions likely abolish repressor-binding sites in MFCS1 and thereby lead to the ectopic Shh expression in the anterior bud. Thus, this study revealed that there are two types' molecular etiologies in preaxitial polydactyl mutations.
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