An Evo-Devo study for mechanisms of early embryogenesis in vertebrates

• Project/Area Number: 22770229
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TAKEUCHI Masaki 独立行政法人理化学研究所, ボディプラン研究グループ, 専門職研究員 (00392019)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 体軸 / オーガナイザー / 三胚葉 / 条鰭類 / 両生類 / siamois / Wntシグナル / nodal / 初期発生 / ポリプテルス / 脊椎動物 / ホメオボックス転写因子

Research Abstract

Xenopus siamois is a homeobox gene cording for a transcriptional mediator of the dorsal Wnt signaling pathway and is necessary for the head formation. Despite its importance in Xenopus development, no siamois-related genes have been found in other taxa. In this study, I found a novel siamois-related gene, dioskouroi, in bichir. The bichir gene, which is different from siamois, plays roles in not only organizer formation but also endoderm differentiation endogenously. These results lead to a hypothesis that the siamois-related protogene in a common ancestor of bony fishes played central role in early embryogenesis.

Research Products

• [Journal Article] Overview of the transcriptome profiles identified in hagfish, shark, and bichir : current issues arising from some nonmodel vertebrate taxa (2011)
  • Author(s): Takechi M., Takeuchi M., Ota K. G., Nishimura O., Mochii M., Itomi K., Adachi1 N., TakahashiM., Fujimoto S., Tarui H., Okabe M., Aizawa S., Kuratani S.
  • Journal Title: Journal of Experimental Zoology Part B : Molecular and Developmental Evolution Volume: 316B Pages: 526-546
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm (2010)
  • Author(s): Kurokawa D., Ohmura T., Ogino H., Takeuchi M., Inoue A., Inoue F., Suda Y., Aizawa S
  • Journal Title: Developmental Biology Volume: 342 Pages: 110-120
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm. (2010)
  • Author(s): Kurokawa D., Ohmura T., Ogino H., Takeuchi M., Inoue A., Inoue F., Suda Y., Aizawa S.
  • Journal Title: Developmental Biology Volume: 342 Pages: 110-120
  • Peer Reviewed
• [Presentation] 脊椎動物における初期発生システムの多様性-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴
  • Organizer: 日本発生生物学会秋季シンポジウム2011
  • Place of Presentation: 岡崎コンファレンスセンター,岡崎市
  • Year and Date: 2011-12-19
• [Presentation] 脊椎動物における初期発生システムの多様性-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴
  • Organizer: 日本発生生物学会秋季シンポジウム2011
  • Place of Presentation: 岡崎コンファレンスセンター(岡崎市)
  • Year and Date: 2011-12-19
• [Presentation] The early embryogenesis of Polypterus(bichirs): insights into the origin and evolution of developmental mechanisms in vertebrates (2011)
  • Author(s): 竹内雅貴,高橋麻衣子,相沢慎一
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: パシフィコ横浜,横浜市
  • Year and Date: 2011-12-08
• [Presentation] The early embryogenesis of Polypterus (bichirs) : insights into the origin and evolution of developmental mechanisms in vertebrates (2011)
  • Author(s): 竹内雅貴, 高橋麻衣子, 相沢慎一
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-08
• [Presentation] 脊椎動物における初期発生システムの進化-原始的条鰭類ポリプテルスの胚発生から考察する- (2011)
  • Author(s): 竹内雅貴,相沢慎一
  • Organizer: 第5回日本ツメガエル研究会
  • Place of Presentation: かんぽの宿本館,熱海市
  • Year and Date: 2011-10-06
• [Presentation] 脊椎動物における初期発生システムの進化-原始的条鰭類ポリプテルスの胚発生から考察する- (2011)
  • Author(s): 竹内雅貴, 相沢慎一
  • Organizer: 第5回日本ツメガエル研究会
  • Place of Presentation: かんぽの宿本館(熱海市)
  • Year and Date: 2011-10-06
• [Presentation] 脊椎動物における初期発生システムの多様性-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴
  • Organizer: 日本動物学会第82回旭川大会2011(招待講演)
  • Place of Presentation: 大雪クリスタルホール,旭川市
  • Year and Date: 2011-09-21
• [Presentation] 脊椎動物における初期発生システムの多様性-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴
  • Organizer: 日本動物学会第82回旭川大会2011
  • Place of Presentation: 大雪クリスタルホール(旭川市)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] 脊椎動物における初期発生システムの進化-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴,相沢慎一
  • Organizer: 日本進化学会2011京都
  • Place of Presentation: 京都大学,京都市
  • Year and Date: 2011-07-30
• [Presentation] 脊椎動物における初期発生システムの進化-原始的条鰭類ポリプテルスの知見から考察する- (2011)
  • Author(s): 竹内雅貴, 相沢慎一
  • Organizer: 日本進化学会2011京都
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-07-30
• [Presentation] The early embryogenesis of Polypterus(bichirs): insights into the origin and evolution of developmental mechanisms in vertebrates (2011)
  • Author(s): Takeuchi M., Takahashi M., Aizawa S.
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists(招待講演)
  • Place of Presentation: 沖縄コンベンションセンター,宜野湾市
  • Year and Date: 2011-05-21
• [Presentation] The early embryogenesis of Polypterus (bichirs) : insights into the origin and evolution of developmental mechanisms in vertebrates (2011)
  • Author(s): Takeuchi M., Takahashi M., Aizawa S.
  • Organizer: 44^<th> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(宜野湾市)(招待講演)
  • Year and Date: 2011-05-21
• [Presentation] 脊椎動物における初期発生の多様性-原始的条鰭類ポリプテルスの知見から- (2010)
  • Author(s): 竹内雅貴,高橋麻衣子,相沢慎一
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: プラザイースト,さいたま市
  • Year and Date: 2010-09-18
• [Presentation] 脊椎動物における初期発生の多様性-原始的条鰭類ポリプテルスの知見から- (2010)
  • Author(s): 竹内雅貴
  • Organizer: 第16回 小型魚類研究会
  • Place of Presentation: さいたま市 プラザイースト
  • Year and Date: 2010-09-18
• [Presentation] The early embryogenesis of Polypterus(bichirs): Insights into the origin and evolution of vertebrate body plans (2010)
  • Author(s): Masaki Takeuchi
  • Organizer: 日本進化学会第12回東京大会(招待講演)
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2010-08-04
• [Presentation] The early embryogenesis of Polypterus (bichirs) : Insights into the origin and evolution of vertebrate body plans (2010)
  • Author(s): Masaki Takeuchi
  • Organizer: 日本進化学会 第12回東京大会
  • Place of Presentation: 東京工業大学 大岡山キャンパス
  • Year and Date: 2010-08-04
• [Presentation] The early embryogenesis of Polypterus(bichirs): Insights into the origin and evolution of vertebrate body plans (2010)
  • Author(s): Takeuchi M., Takahashi M., Aizawa S.
  • Organizer: 2nd Joint Meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: Institute Pasteur, Paris, France
  • Year and Date: 2010-05-27
• [Presentation] The early embryogenesis of Polypterus (bichirs) : Insights into the origin and evolution of vertebrate body plans (2010)
  • Author(s): Takeuchi M., Takahashi M., Aizawa S.
  • Organizer: 2nd Joint Meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: Institute Pasteur, Paris, France
  • Year and Date: 2010-05-27
• [Remarks] Vertebrate Time Capsule
  • URL: http://transcriptome.cdb.riken.go.jp/vtcap/
• [Remarks] The Rising Generation of Evo-Devo Biologist
  • URL: http://www.evo-devo.net/posters.html
• [Remarks] Vertebrate Time Capsule
  • URL: http://transcriptome.cdb.riken.go.jp/vtcap/
• [Remarks] The Rising Generation of Evo-Devo Biologist
  • URL: http://www.evo-devo.net/posters.html
• [Remarks]
  • URL: http://www.evo-devo.net/posters.html