Evolutionary analysis of olfaction in two genetically diverged medaka strains

• Project/Area Number: 22770236
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Osaka Medical College
• Principal Investigator: HASHIGUCHI Yasuyuki 大阪医科大学, 医学部, 助教 (70436517)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: メダカ / 嗅覚受容体 / 魚類 / 分子進化 / 定量PCR / RLM-RACE / 行動解析 / 進化 / 遺伝子発現

Research Abstract

To understand the evolutionary mechanisms of fish olfaction, genetical and behavioral differences in two genetically divergent inbred strains(Hd-rR and HNI) of medaka(Oryzias latipes) were characterized by comparative analyses. Phylogenetic analysis of olfactory receptor(OR) genes has shown that most OR genes were common to the two strains, but several strain-specific gene duplications and pseudogenizations were also detected. In addition, qPCR analysis has identified expression differences of OR genes between the two strains. In behavioral analysis, an assay system that measures behavioral responses(i. e. attraction and avoidance) of medaka to amino acid odorants has been established. The results of this study provide valuable insights into the adaptive evolution of olfaction in teleost fishes.

Research Products

• [Journal Article] Characterization of squamate olfactory receptor genes and their transcripts by the high-throughput sequencing approach (2012)
  • Author(s): Dehara Y, Hashiguchi Y, Matsubara K, Yanai T, Kubo M and Kumazawa Y
  • Journal Title: Genome Biology and Evolution Volume: 4(4) Issue: 4 Pages: 602-616
  • DOI: 10.1093/gbe/evs041
  • Peer Reviewed
• [Journal Article] 西表島におけるドジョウの危機的生息状況と遺伝的特異性 (2012)
  • Author(s): 鹿野雄一、中島淳、水谷宏、仲里裕子、仲里長浩、楫善継、黄亮亮、西田伸、橋口康之
  • Journal Title: 魚類学雑誌 Volume: 5 9 Pages: 37-43
  • NAID: 130005003671
  • URL: http://www.fish-isj.jp/publication/index.html
• [Journal Article] Mitochondrial genomes of two African gechos of genus Hemitheconyx (Squamata : Eublepharidae) (2012)
  • Author(s): Jonniaux, P, Hashiguchi Y, Kumazawa Y
  • Journal Title: Mitochondrial DNA Volume: (印刷中)(未定)
  • Peer Reviewed
• [Journal Article] 西表島におけるドジョウの危機的生息状況と遺伝的特異性 (2012)
  • Author(s): 鹿野雄一、中島淳、水谷宏, 他6名
  • Journal Title: 魚類学雑誌 Volume: 59 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Purification and characterization of tributyltin-binding protein of tiger puffer, Takifugu rubripes (2011)
  • Author(s): Oba Y., Yamauchi A., Hashiguchi Y., Satone, H., Miki, S., Nassef, M., Shimasaki, Y., Kitano, T., Nakao, M., Kawabata, S-i., Honjo, T., Oshima, Y.
  • Journal Title: Comparative Biochemistry and Physiology, Part C Volume: 153 Pages: 17-23
• [Journal Article] 日本のイトヨ自然集団における嗅覚受容体遺伝子ファミリーの適応進化 (2010)
  • Author(s): 橋口康之・西田睦
  • Journal Title: 月刊海洋 Volume: 42 Pages: 372-378
  • URL: http://www.kaiyo-chikyu.com/
• [Journal Article] Mitochondrial genomes of two African gechos of genus Hemitheconyx(Squamata : Eublepharidae)
  • Author(s): Jonniaux, P, Hashiguchi Y, Kumazawa Y.
  • Journal Title: Mitochondrial DNA Volume: (印刷中)
• [Presentation] フグ科魚類におけるフグ毒結合タンパク質の分子進化 (2012)
  • Author(s): 橋口康之, L. Jaeman, 白石真、小松正治、三木志津帆、島崎洋平、日下部宣宏、大嶋雄治
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] Nucleotide and copy number differences of olfactory receptor(OR) genes in two inbred strains of medaka fish (2011)
  • Author(s): Y. Hashiguchi
  • Organizer: SMBE 2011
  • Place of Presentation: Kyoto University
  • Year and Date: 2011-07-26
• [Presentation] Nucleotide and copy number differences of olfactory receptor (OR) genes in two inbred strains of medaka fish (2011)
  • Author(s): Yasuyuki Hashiguchi
  • Organizer: SMBE 2011
  • Place of Presentation: Kyoto University
  • Year and Date: 2011-07-26
• [Presentation] 魚類ゲノムにおける「逆転写に由来する遺伝子重複」の網羅的探索 (2010)
  • Author(s): 橋口康之
  • Organizer: 日本遺伝学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-21
• [Presentation] A comparison of odorant receptor gene repertoires between two genetically different strains of medaka fish (2010)
  • Author(s): Y Hashiguchi
  • Organizer: International Symposium of Biodiversity Sciences
  • Place of Presentation: Nagoya City University
  • Year and Date: 2010-08-01
• [Book] 現代の生態学7エコゲノミクス-遺伝子からみた適応-(森長真一・工藤洋編) (2012)
  • Author(s): 橋口康之(分担執筆)
  • Publisher: 共立出版(印刷中)(未定)
• [Book] 生物の事典 (2010)
  • Author(s): 橋口康之
  • Publisher: 朝倉書店
• [Book] 生物の事典(石原勝敏・末光隆志編) (2010)
  • Author(s): 橋口康之(分担執筆)
  • Total Pages: 6
  • Publisher: 朝倉書店
• [Book] 現代の生態学7エコゲノミクス-遺伝子からみた適応-
  • Author(s): 橋口康之
  • Publisher: 共立出版
• [Remarks]
  • URL: https://sites.google.com/site/hashiyuki0909/home