Evolutionary analysis of olfaction in two genetically diverged medaka strains
| Project/Area Number |
22770236
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Evolutionary biology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | メダカ / 嗅覚受容体 / 魚類 / 分子進化 / 定量PCR / RLM-RACE / 行動解析 / 進化 / 遺伝子発現 |
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| Research Abstract |
To understand the evolutionary mechanisms of fish olfaction, genetical and behavioral differences in two genetically divergent inbred strains(Hd-rR and HNI) of medaka(Oryzias latipes) were characterized by comparative analyses. Phylogenetic analysis of olfactory receptor(OR) genes has shown that most OR genes were common to the two strains, but several strain-specific gene duplications and pseudogenizations were also detected. In addition, qPCR analysis has identified expression differences of OR genes between the two strains. In behavioral analysis, an assay system that measures behavioral responses(i. e. attraction and avoidance) of medaka to amino acid odorants has been established. The results of this study provide valuable insights into the adaptive evolution of olfaction in teleost fishes.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Purification and characterization of tributyltin-binding protein of tiger puffer, Takifugu rubripes2011
Author(s)
Oba Y., Yamauchi A., Hashiguchi Y., Satone, H., Miki, S., Nassef, M., Shimasaki, Y., Kitano, T., Nakao, M., Kawabata, S-i., Honjo, T., Oshima, Y.
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Journal Title
Comparative Biochemistry and Physiology, Part C
Volume: 153
Pages: 17-23
Related Report
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