Study of the self-incompatibility system in Arabidopsis thaliana

• Project/Area Number: 22780002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: Mie University
• Principal Investigator: SUWABE Keita 三重大学, 大学院・生物資源学研究科, 准教授 (50451612)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: シロイヌナズナ / 自家不和合性 / 自他認識下流因子 / シグナル伝達 / 柱頭 / 近縁野生種 / トランスクリプトーム / 次世代シーケンサー / 自他認識反応 / 突然変異 / ゲノム / 雄性配偶子 / 花粉

Research Abstract

Self-incompatibility is defined as the inability of a fertile hermaphrodite plant to produce zygotes after self-pollination. In this research, we elucidated causal reasons of a disruption of the self-incompatibility system in Arabidopsis thaliana. Also, we obtained gene information involving in a self-incompatibility network, by the Laser Microdissection-Next Generation Sequencing transcriptome analysis of Arabidopsis papilla cell.

Research Products

• [Journal Article] Comprehensive network analysisof anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays (2011)
  • Author(s): Koichiro Aya, Go Suzuki, Keita Suwabe, Tokunori Hobo, Hirokazu Takahashi, Katsuhiro Shiono, Kentaro Yano, Nobuhiro Tsutsumi, Mikio Nakazono, Yoshiaki Nagamura, Makoto Matsuoka, Masao Watanabe
  • Journal Title: PLoS ONE Volume: Vol.6 Pages: 26162-26162
  • Peer Reviewed
• [Journal Article] Oryza Express : an integrated database of gene expression networks and omics annotations in rice (2011)
  • Author(s): Kazuki Hamada, Kohei Hongo, Keita Suwabe, Akifumi Shimizu, Taishi Nagayama, Reina Abe, Shunsuke Kikuchi, Naoki Yamamoto, Takaaki Fujii, Koji Yokoyama, Hiroko Tsuchida, Kazumi Sano, Takako Mochizuki, Nobuhiko Oki, Youko Horiuchi, Masahiro Fujita, Masao Watanabe, Makoto Matsuoka, Nori Kurata, Kentaro Yano
  • Journal Title: Plant & Cell Physiology Volume: Vol.52 Pages: 220-229
  • NAID: 10029708412
  • Peer Reviewed
• [Journal Article] イネにおけるマイクロアレイ解析の現状と今後の展望 (2011)
  • Author(s): 安益公一郎、諏訪部圭太
  • Journal Title: 化学と生物 Volume: Vol.49 Pages: 261-268
  • NAID: 10028117266
• [Journal Article] Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays (2011)
  • Author(s): Koichiro Aya, et al
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Microstructure of a Brassica rapa genome segment homoeologous to the resistance gene cluster on Arabidopsis chromosome 4 (2011)
  • Author(s): Keita Suwabe, et al
  • Journal Title: Breeding Science Volume: (掲載確定)
  • NAID: 10030434685
  • Peer Reviewed
• [Journal Article] Oryza Express : an integrated database of gene expression networks and omics annotations in rice. (2011)
  • Author(s): Kazuki Hamada, et al.
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 220-229
  • Peer Reviewed
• [Journal Article] Achievement of genetics in plant reproduction research : the past decade for the coming decade (2010)
  • Author(s): Keita Suwabe, Go Suzuki, Masao Watanabe
  • Journal Title: Genes & Genetic Systems Volume: Vol.85 Pages: 297-310
  • NAID: 10029515450
  • Peer Reviewed
• [Journal Article] Achievement of genetics in plant reproduction research : the past decade for the coming decade. (2010)
  • Author(s): Keita Suwabe, et al.
  • Journal Title: Genes & Genetic Systems Volume: 85 Pages: 297-310
  • NAID: 10029515450
  • Peer Reviewed
• [Journal Article] Microstructure of a Brassica rapa genome segment homoeologous to the resistance gene cluster on Arabidopsis chtromosome 4
  • Author(s): Keita Suwabe, Go Suzuki, Tsukasa Nunome, Katsunori Hatakeyama, Yasuhiko Mukai, Hiroyuki Fukuoka, Satoru Matsumoto
  • Journal Title: Breeding Science
  • Peer Reviewed
• [Presentation] アブラナ科植物における分子遺伝学研究基盤の構築と育種学的研究 (2012)
  • Author(s): 諏訪部圭太
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] レーザーマイクロダイセクションによるアブラナ科乳頭細胞の単離法の構築 (2012)
  • Author(s): 松田智貴、大坂正明、藤岡智明、坂園聡美、高橋宏和、中園幹生、岩野恵、高山誠司、鈴木剛、渡辺正夫、諏訪部圭太
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] LM-NGS法によるアブラナ科乳頭細胞のトランスクリプトーム解析 (2012)
  • Author(s): 大坂正明、松田智貴、藤岡智明、坂園聡美、高橋宏和、中園幹生、岩野恵、高山誠司、鈴木剛、諏訪部圭太、渡辺正夫
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] イネ雄性生殖器官におけるsmall RNAの網羅的解析 (2012)
  • Author(s): 藤岡智明、浜田和輝、本郷耕平、矢野健太郎、増子潤美、牧野周、前忠彦、諏訪部圭太、鈴木剛、渡辺正夫
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] Laser Microdissection-NextGeneration Sequence Transcriptome Analysisin the Papilla Cell of Brassica rapa (2012)
  • Author(s): Masaaki Osaka, Tomoki Matsuda, Yuki Kobushiro, Kaori Nagasaka, Hirokazu Takahashi, Mikio Nakazono, Go Suzuki, Yong-Pyo Lim, Keita Suwabe, Masao Watanabe
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] Analysis of Pollen Behavior on Brassica Self-Incompatibility (2012)
  • Author(s): Kenichiro Hiroi, Keita Suwabe, Go Suzuki, Masao Watanabe
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] NGS Transcriptome of Small RNAs in Rice Male Gametophyte Development (2012)
  • Author(s): Tomoaki Fujioka, Kazuki Hamada, Kohei Hongo, Kentaro Yano, Hiromi Masuko-Suzuki, Kaori Yamamura, Amane Makino, Tadahiko Mae, Keita Suwabe, Go Suzuki, Masao Watanabe
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] The pollen specific LIM gene for pollen germination and pollen tube growth in Arabidopsis thaliana (2012)
  • Author(s): Keisuke Sudo, Jong-In Park, Keita Suwabe, Go Suzuki, Masao Watanabe
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] Improving the Agrobacterium-Mediated Transformation Protocol for Brachypodium Distachyon (2012)
  • Author(s): Seiya Goto, Tomoaki Fujioka, Takahiro Akimoto, Hiromi Masuko-Suzuki, Masao Watanabe, Go Suzuki, Silvia C. Alves, Keita Suwabe
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] An integrated Database for Gene Expression Networks in Rice (2012)
  • Author(s): Kazuki Hamada, Kai Fukazawa, Taishi Nagayama, Koji Yokoyama, Hiroko Tsuchida, Kaori Igarashi, Keita Suwabe, Masao Watanabe, Makoto Matsuoka, Nori Kurata, Kentaro Yano, Oryza Express
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] Laser Microdissection-Next Generation Sequence Transcriptome Analysis in the Papilla Cell of Brassica rapa (2012)
  • Author(s): Masaaki Osaka, ら
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] Oryza Express : An integrated Database for Gene Expression Networks in Rice (2012)
  • Author(s): Kazuki Hamada, ら
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, USA
• [Presentation] レーザーマイクロダイセクションによるアブラナ科乳頭細胞の単離法の構築 (2012)
  • Author(s): 松田智貴, ら
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] LM-NGS法によるアブラナ科乳頭細胞のトランスクリプトーム解析 (2012)
  • Author(s): 大坂正明, ら
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] イネ雄性生殖器官におけるsmall RNAの網羅的解析 (2012)
  • Author(s): 藤岡智明, ら
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮大学
• [Presentation] アブラナ科植物における分子遺伝学研究基盤の構築と育種学的研究 (2012)
  • Author(s): 諏訪部圭太
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮大学(招待講演)
• [Presentation] Significance of genetic resources for self-incompatibility research in Brassica and Brassicaceae (2011)
  • Author(s): Keita Suwabe
  • Organizer: Importance of Germplasm in Crop Genomics and Breedeing
  • Place of Presentation: Chungnam National University, Korea
  • Year and Date: 2011-08-09
• [Presentation] Significance of genetic resources for self-incompatibility research in Brassica and Brassicaceae (2011)
  • Author(s): Keita Suwabe
  • Organizer: Importance of Germplasm in Crop Genomics and Breedeing
  • Place of Presentation: Daejeon, Korea(招待講演)
  • Year and Date: 2011-08-09
• [Presentation] アブラナ科植物自家不和合性反応における花粉動態の解析 (2011)
  • Author(s): 広井健一郎、高山誠司、鈴木剛、諏訪部圭太、渡辺正夫
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
• [Presentation] イネの遺伝子発現ネットワークとオミックス情報統合データベース (2011)
  • Author(s): 濱田和輝、本郷耕平、諏訪部圭太、清水顕史、長山大志、阿部玲奈、菊池俊介、山本直樹、藤井貴朗、横山幸治、土田博子、佐野和美、望月孝子、大木信彦、堀内陽子、藤田雅丈、渡辺正夫、松岡信、倉田のり、矢野健太郎
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 横浜市立大学
• [Presentation] アブラナ科植物自家不和合性反応における花粉動態の解析 (2011)
  • Author(s): 広井健一郎, ら
  • Organizer: 第120回日本育種学会講演会
  • Place of Presentation: 福井県立大学
• [Presentation] Oryza Express:イネの遺伝子発現ネットワークとおミックス情報統合データベース (2011)
  • Author(s): 濱田和輝, ら
  • Organizer: 第119回日本育種学会講演会
  • Place of Presentation: 横浜市立大学(神奈川県)
• [Presentation] アブラナ科自家和合変異系統の分子遺伝学的解析と連鎖地図へのマッピング (2010)
  • Author(s): 大坂正明、五十川祥代、白澤彰、菊田利奈、小松聡、新倉聡、高田美信、柴博史、磯貝彰、高山誠司、鈴木剛、諏訪部圭太、渡辺正夫
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
• [Presentation] ハクサイ根こぶ病抵抗性遺伝子Crr1のマップベースクローニング (2010)
  • Author(s): 畠山勝徳、諏訪部圭太、加藤丈幸、布目司、福岡浩之、松元哲
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
• [Presentation] イネの遺伝子発現ネットワーク解析とデータベース構築 (2010)
  • Author(s): 濱田和輝、本郷耕平、山本直樹、藤井貴朗、望月孝子、諏訪部圭太、倉田のり、矢野健太郎
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
• [Book] The Use of Omics Databases for Plant (2011)
  • Author(s): Ayako Suzuki, Keita Suwabe, Kentaro Yano
  • Publisher: CRC Press
• [Book] Sustainable agriculture and new biotechnologies (2011)
  • Author(s): Ayako Suzuki, et al
  • Publisher: CRC Press
• [Remarks] <受賞>日本育種学会奨励賞、2012年3月29日日本育種学会第118回講演会優秀発表賞、2010年9月24-25日