A transposon suppressor Dart-cancellar in wild rice
Project/Area Number |
22780007
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Breeding science
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Research Institution | National Institute for Basic Biology |
Principal Investigator |
TSUGANE Kazuo 基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 植物分子育種 / トランスポソン / イネ / 抑制因子 / 野生イネ / DNAトランスポゾン |
Research Abstract |
Whole rice genome, which transposon content is 35 %, was sequenced with high accuracy and informative for transposon research. An active rice transposon nDart1-0 element was identified as a causative element for the mutable virescent line that shows green sectors in the virescent yellow leaves. The transposition of nonautonomous nDart1-0 element requires transposase supplied from an autonomous aDart1-27 element. An introduced aDart1-27 element from other variety by crossing has kept the active state. However, a hybrid line obtained by interspecific cross between an African wild rice species, Oryza longistaminata, and a mutable virescent line showed the only green leaf phenotype even though it carried both the nDart1-0 and aDart1-27 elements. Genetic analysis indicated the inhibition of nDart1-0 transposition was caused by a factor derived from Oryza longistaminata. It was revealed that the inhibitory factor regulated transposase mRNA in a posttranscriptional manner
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Examination of transpositional activity of nDart1 at different stages of rice development2011
Author(s)
Hayashi-Tsugane, M., Maekawa, M., Qian, Q., Kobayashi H., Iida, S., Tsugane, K.
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Journal Title
Genes Genet Syst
Volume: 86
Pages: 215-219
NAID
Related Report
Peer Reviewed
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