| Project/Area Number |
22780007
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
TSUGANE Kazuo 基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 植物分子育種 / トランスポソン / イネ / 抑制因子 / 野生イネ / DNAトランスポゾン |
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| Research Abstract |
Whole rice genome, which transposon content is 35 %, was sequenced with high accuracy and informative for transposon research. An active rice transposon nDart1-0 element was identified as a causative element for the mutable virescent line that shows green sectors in the virescent yellow leaves. The transposition of nonautonomous nDart1-0 element requires transposase supplied from an autonomous aDart1-27 element. An introduced aDart1-27 element from other variety by crossing has kept the active state. However, a hybrid line obtained by interspecific cross between an African wild rice species, Oryza longistaminata, and a mutable virescent line showed the only green leaf phenotype even though it carried both the nDart1-0 and aDart1-27 elements. Genetic analysis indicated the inhibition of nDart1-0 transposition was caused by a factor derived from Oryza longistaminata. It was revealed that the inhibitory factor regulated transposase mRNA in a posttranscriptional manner
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