| Project/Area Number |
22780009
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
TSUKAZAKI Hikaru 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所・野菜育種・ゲノム研究領域, 主任研究員 (30355622)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タマネギ / 連鎖地図 / ゲノム比較 / QTL解析 / 着色性 / DFR / ネギ / シンテニー |
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| Research Abstract |
Linkage maps have been developed for bulb onion(Allium cepa L.) and bunching onion(A. fistulosum L.). It is considered that a high level of macrosynteny exists between these 2 species since they belong to the section Cepa and have the same chromosome number(2n=16). However, a detail comparison has not evaluated because there are few common markers onto the maps of both species. The objective of this study was to evaluate synteny at the genome-wide level between bunching onion and bulb onion. As a first step, we developed a basal linkage map in F_2 population between yellow bulb onion and red shallot. Linkage map consists of 11 linkage groups with 164 markers covering 1, 040 cM. Ten linkage groups were assigned to eight chromosomes with 65 markers. Although both SNP markers(F3' H and DFR) were located on the Chr. 7, two trait markers, Pg and Ant1, were closely linked to DFR. A QTL for bulb pigmentation was also detected on the Chr. 7.All individuals homozygous for onion allele at DFR loci showed no pigmentation in F_2 individuals. The degree of red pigmentation became significantly darkened by replacing shallot allele at DFR in both F_2 and F_<2 : 3>. Therefore, DFR is one of key genes involved in bulb color in this population.
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