Shed light on the flavonoid accumulation of fruit
| Project/Area Number |
22780029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2010-10-20 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 果樹 / フラボノイド / アントシアニン / プロアントシアニジン / シキミ酸 / カキ / ブドウ / ABA / 没食子酸 / ガレート / 生合成 / 転写因子 / 形質転換 |
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| Research Abstract |
In order to characterize DkDHD/SDH, DkSCPL1 and DkSCPL2, which is supposed to be involved in proanthocyanidin accumulation in persimmon fruit, we tried transformation of persimmon leaf with these genes using Agrobacterium tumefaciens, and also production of DkDHD/SDH with Escherichia coli BL21(DE3) for complementation assay. We could not obtain clear results for DkSCPLs. As for DkDHD/SDH, transformation by antisense DkDHD/SDH reduce the production of proanthocyanidin, and complementation of BL21 delta aroE::KanR (DE3) with pET3a-DkDHDSDH shows this protein works as SDH in shikimic pathway.
Secondly, we applied ABA and IAA to red grape cultivars around varaison to clear the effect of these plant hormones at anthocyanin accumulation of berry skin. The results indicated that the ABA treatment induces the accumulation of blue-purple anthocyanins via MybA transcription factor, while the IAA treatment reduces it.
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Report
(3 results)
Research Products
(10 results)
