• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Shed light on the flavonoid accumulation of fruit

Research Project

Project/Area Number 22780029
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionIshikawa Prefectural University

Principal Investigator

KATAYAMA-IKEGAMI Ayako  石川県立大学, 生物資源環境学部, 准教授 (00549339)

Project Period (FY) 2010-10-20 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords果樹 / フラボノイド / アントシアニン / プロアントシアニジン / シキミ酸 / カキ / ブドウ / ABA / 没食子酸 / ガレート / 生合成 / 転写因子 / 形質転換
Research Abstract

In order to characterize DkDHD/SDH, DkSCPL1 and DkSCPL2, which is supposed to be involved in proanthocyanidin accumulation in persimmon fruit, we tried transformation of persimmon leaf with these genes using Agrobacterium tumefaciens, and also production of DkDHD/SDH with Escherichia coli BL21(DE3) for complementation assay. We could not obtain clear results for DkSCPLs. As for DkDHD/SDH, transformation by antisense DkDHD/SDH reduce the production of proanthocyanidin, and complementation of BL21 delta aroE::KanR (DE3) with pET3a-DkDHDSDH shows this protein works as SDH in shikimic pathway.
Secondly, we applied ABA and IAA to red grape cultivars around varaison to clear the effect of these plant hormones at anthocyanin accumulation of berry skin. The results indicated that the ABA treatment induces the accumulation of blue-purple anthocyanins via MybA transcription factor, while the IAA treatment reduces it.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report

Research Products

(10 results)

All 2013 2012 2011 2010

All Journal Article Presentation

  • [Journal Article] 中国の完全甘ガキ後代における甘渋性と果形との関連性2013

    • Author(s)
      片山(池上)礼子・米森敬三・佐藤明彦・山田昌彦・羽生 剛・北島 宜
    • Journal Title

      園芸学研究

      Volume: 12 Pages: 29-34

    • URL

      https://www.jstage.jst.go.jp/article/hrj/12/1/12_29/_pdf

    • Related Report
      2013 Final Research Report
  • [Journal Article] 中国の完全甘ガキ後代における甘渋性と果形との関連性2013

    • Author(s)
      片山(池上)礼子・米森敬三・佐藤明彦・山田昌彦・羽生 剛・北島 宜.
    • Journal Title

      園芸学研究.

      Volume: 12 Pages: 29-34

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal abscisic acid signal and a basic leucine zipper transcription factor, DkbZIP5, regulate proanthocyanidin biosynthesis in persimmon fruit2012

    • Author(s)
      Akagi, T., A.Katayama-Ikegami, S.Kobayashi, A.Sato, A.Kono, K.Yonemori
    • Journal Title

      Plant Physiol

      Volume: 158 Pages: 1089-1102

    • DOI

      10.1104/pp.111.191205

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Molecular Markers Linked to the Allele Associated with the Non-astringent Trait of the Chinese Persimmon (Diospyros kaki Thunb.)2011

    • Author(s)
      Ikegami, A., S. Eguchi, T. Akagi, A. Sato, M. Yamada, S. Kanzaki, A .Kitajima, and K. Yonemori
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 80 Issue: 2 Pages: 150-155

    • DOI

      10.2503/jjshs1.80.150

    • NAID

      130004510706

    • ISSN
      1882-3351, 1882-336X
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] Effects of seasonal temperature changes on DkMyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (Diospyros kaki Thunb.) fruit2011

    • Author(s)
      Akagi T, Tsujimoto T, Ikegami A, Yonemori K
    • Journal Title

      Planta

      Volume: 233 Pages: 883-94

    • DOI

      10.1007/s00425-010-1346-z

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] Proanthocyanidin biosynthesis of persimmon (Diospyros kaki Thunb.) fruit2011

    • Author(s)
      Akagi, T., A. Katayama-Ikegami, and K. Yonemori
    • Journal Title

      Sciencia Horticulaturae

      Volume: 130 Pages: 373-380

    • DOI

      10.1016/j.scienta.2011.07.021

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] Condensed Tannin Composition Analysis in Persimmon (Diospyros kaki Thunb.) Fruit by Acid Catalysis in the Presence of Excess Phloroglucinol2010

    • Author(s)
      Akagi, T., Y. Suzuki, A. Ikegami, H. Kamitakahara, T. Takano, F. Nakatsubo, and K. Yonemori
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 79 Issue: 3 Pages: 275-281

    • DOI

      10.2503/jjshs1.79.275

    • NAID

      130004726129

    • ISSN
      1882-3351, 1882-336X
    • Related Report
      2013 Final Research Report
  • [Journal Article] DkMyb2 wound-induced transcription factor of persimmon (Diospyros kaki Thunb.), contributes to proanthocyanidin regulation2010

    • Author(s)
      Akagi T, A. Ikegami, T. Tsujimoto, and K. Yonemori
    • Journal Title

      Planta

      Volume: 232 Pages: 1045-59

    • DOI

      10.1007/s00425-010-1241-7

    • Related Report
      2013 Final Research Report
  • [Presentation] 赤色系ブドウにおける植物生長調節物質(アブシジン酸およびオーキシン)処理の影響2011

    • Author(s)
      澁谷佳奈・高居恵愛・片山礼子
    • Organizer
      平成23年度園芸学会北陸支部会
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] 花成関与遺伝子であるFT およびTFL1によるカキおよびキウイフルーツの形質転換2011

    • Author(s)
      武石浩二・高居恵愛・片山礼子・田尾龍太郎
    • Organizer
      平成23年度園芸学会北陸支部会
    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-11-29   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi