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Formation mechanism of sperm dimorphism in higher plant

Research Project

Project/Area Number 22780034
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

HIRANO Tomonarai  独立行政法人理化学研究所, イオンビーム育種研究チーム, 研究員 (80455584)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords花粉 / 雄原細胞 / 精細胞 / 遺伝子発現 / EST 解析 / 微細操作 / 重イオンビーム / EST解析 / DNA2本鎖切断 / DNA二本鎖切断
Research Abstract

In Cyrtanthus mackenii, the sperm cells showed structural dimorphism, and differences in microtubule accumulation between the pair of sperm cells arose during the process of cortical microtubule reorganization. As results from EST analysis for elucidation of molecular mechanisms on the sperm dimorphism, the candidate genes with changes in expression during sperm cell formation were listed up. Male gametes irradiated heavy ion beam had a potential to use for the study of sperm cell formation. It was also revealed that the generative cells recognized and managed genomic lesions during the pollen tube growth.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (5 results) Book (2 results) Remarks (1 results)

  • [Journal Article] DNA damage response in male gametes of Cyrtanthus mackenii during pollen tube growth.2013

    • Author(s)
      T. Hirano, K. Takagi, Y. Hoshino, T. Abe
    • Journal Title

      AoB PLANTS

      Volume: 5 Issue: 0 Pages: 1-10

    • DOI

      10.1093/aobpla/plt004

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid evaluation of effective linear energy transfer in heavy-ion mutagenesis of <i>Arabidopsis thaliana</i>2012

    • Author(s)
      Y. Kazama, L. Ma, T. Hirano, S. Ohbu, Y. Shirakawa, S. Hatakeyama, S. Tanaka, T. Abe
    • Journal Title

      Plant Biotechnology

      Volume: 29 Issue: 5 Pages: 441-445

    • DOI

      10.5511/plantbiotechnology.12.0921a

    • NAID

      130002500889

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular nature of mutations induced by high-LET irradiation with argon and carbon ions in Arabidopsis thaliana.2012

    • Author(s)
      T. Hirano, Y. Kazama, S. Ohbu, Y. Shirakawa, L. Yang, T. Kambara, N. Fukunishi, T. Abe
    • Journal Title

      Mutation Research: Fundamental and Molecular Mechanisms of Mutagenesis

      Volume: 735 Issue: 1-2 Pages: 19-31

    • DOI

      10.1016/j.mrfmmm.2012.04.010

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana.2012

    • Author(s)
      Fujiwara, M.T., Yoshioka, Y., Hirano, T., Kazama. Y., Abe, T., Hayashi, K., Itoh, R.D.
    • Journal Title

      Plant Signaling Behavior

      Volume: vol. 7 (1) Issue: 1 Pages: 34-37

    • DOI

      10.4161/psb.7.1.18484

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana.2011

    • Author(s)
      Y. Kazama, T. Hirano, H. Saito, Y. Liu, S. Ohbu, Y. Hayashi, T. Abe
    • Journal Title

      BMC Plant Biology

      Volume: 11 Issue: 1 Pages: 161-161

    • DOI

      10.1186/1471-2229-11-161

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii.2010

    • Author(s)
      T. Hirano, Y. Hoshino
    • Journal Title

      Sexual Plant Reproduction

      Volume: 23 Issue: 2 Pages: 153-162

    • DOI

      10.1007/s00497-009-0123-2

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii2010

    • Author(s)
      Tomonari Hirano
    • Journal Title

      Sexual Plant Reproduction

      Volume: 23 Pages: 153-162

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 重イオンビーム誘発突然変異に対するLET効果の解析2011

    • Author(s)
      平野智也
    • Organizer
      第29回日本植物細胞分子生物学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 重イオンビームを用いたキルタンサス雄性配偶子のDNA損傷応答解析2011

    • Author(s)
      平野智也
    • Organizer
      園芸学会
    • Place of Presentation
      宇都宮大学農学部(栃木県宇都宮市)
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 重イオンビーム誘発突然変異に対するLET効果の解析2011

    • Author(s)
      平野智也, 風間裕介, 大部澄江, 白川侑希, 阿部知子
    • Organizer
      第29回日本植物細胞分子生物学会大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of DNA damage response in male gametes of Cyrtanthus mackenii during pollen tube growth2011

    • Author(s)
      T. Hirano, K. Takagi, Y. Hoshino, T. Abe
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 重イオンビームを用いたキルタンサス雄性配偶子のDNA 損傷応答解析2011

    • Author(s)
      平野智也, 高城啓一, 星野洋一郎, 阿部知子
    • Organizer
      園芸学会平成23 年度春季大会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2012 Final Research Report
  • [Book] Pollen: Structure, Types and Effects2010

    • Author(s)
      T. Hirano, Y. Hoshino
    • Publisher
      Nova Science Publishers
    • Related Report
      2012 Final Research Report
  • [Book] Pollen : Structure, Types and Effects2010

    • Author(s)
      Tomonari Hirano
    • Publisher
      Nova Science Publishers
    • Related Report
      2010 Annual Research Report
  • [Remarks] 山路達也の「エコ技術研究者に訊く」,重イオンビームが生物進化を加速する

    • URL

      http://archive.wiredvision.co.jp/blog/yamaji/201003/201003121701.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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