Development of a high-frequency gene targeting method in Magnaporthe oryzae
| Project/Area Number |
22780038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Meiji University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | イネいもち病菌 / 相同組換え / 遺伝子ターゲッティング / いもち病菌 |
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| Research Abstract |
For investigation of gene functions, several strategies have been established. Of these, gene targeting is generally thought to be the most powerful method. Gene targeting is achieved through the homologous recombination between endogenous genome and foreign marker. However, the frequency of homologous recombination in Magnaporthe oryzae is low. To raise the frequency of homologous recombination, we applied double-strand DNA breaks in target gene using some nucleases that are used in other organisms. Here, we constructed a detection marker system of homologous recombination in M. oryzae. We also revealed that treatment of nucleases increase efficiency of homologous recombination and established on optimal condition.
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Report
(3 results)
Research Products
(21 results)
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[Presentation] Identification of deacetylase genes that determine the side-chain modification patterns of trichothecenes in the late biosynthetic grid of type B trichothecenes2011
Author(s)
K. Maeda, T. Tokai, M. Sato, S. Hyodo, N. Takahashi-Ando, S. Ohsato, M. Fujimura, T. Kamakura, K. Yoneyama, M. Yoshida, M. Kimura
Organizer
International Union of Microbiological Societies 2011 Congress_XIII, International Congress of Mycology
Place of Presentation
札幌,札幌コンベンションセンター/札幌市産業振興センター
Year and Date
2011-09-10
Related Report
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