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Development of a high-frequency gene targeting method in Magnaporthe oryzae

Research Project

Project/Area Number 22780038
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionMeiji University

Principal Investigator

OHSATO Shuichi  明治大学, 農学部, 講師 (30533228)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsイネいもち病菌 / 相同組換え / 遺伝子ターゲッティング / いもち病菌
Research Abstract

For investigation of gene functions, several strategies have been established. Of these, gene targeting is generally thought to be the most powerful method. Gene targeting is achieved through the homologous recombination between endogenous genome and foreign marker. However, the frequency of homologous recombination in Magnaporthe oryzae is low. To raise the frequency of homologous recombination, we applied double-strand DNA breaks in target gene using some nucleases that are used in other organisms. Here, we constructed a detection marker system of homologous recombination in M. oryzae. We also revealed that treatment of nucleases increase efficiency of homologous recombination and established on optimal condition.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Disinfection using ozone microbubbles to inactivate Fusarium oxysporum f. sp. melonis and Pectobacterium carotovorum subsp. Carotovorum2011

    • Author(s)
      F. Kobayashi, H. Ikeura, S. Ohsato, T. Goto, M Tamaki
    • Journal Title

      Crop protection

      Volume: 30 Pages: 1514-1518

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disinfection using ozone microbubbles to inactivate Fusarium oxysporum f.sp.melonis and Pectobacterium carotovorum subsp.Carotovorum2011

    • Author(s)
      F.Kobayashi, H.Ikeura, S.Ohsato, T.Goto, M.Tamaki
    • Journal Title

      Crop Protection

      Volume: 30 Issue: 11 Pages: 1514-1518

    • DOI

      10.1016/j.cropro.2011.07.018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Appearance of a new leaf rot disease on common ice plant2010

    • Author(s)
      Maeda K, Kurahashi Y, Ohsato S, and Yoneyama K
    • Journal Title

      Journal of General Plant Pathology

      Volume: 76 Pages: 303-309

    • NAID

      10029719952

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Appearance of a new leaf rot disease on common ice plant.2010

    • Author(s)
      Maeda K, Kurahashi Y, Ohsato S, Yoneyama K
    • Journal Title

      Journal of General Plant Pathology

      Volume: VOL.76 Pages: 303-309

    • NAID

      10029719952

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA二本鎖切断はイネいもち病菌における遺伝的多様性を誘導する2012

    • Author(s)
      荒添貴之, 大里修一, 有江力, 米山勝美, 桑田茂
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イネいもち病菌におけるジンクフィンガーヌクレアーゼによる相同組換えの誘導2012

    • Author(s)
      用之丸哲也, 荒添貴之, 大里修一, 有江力, 桑田茂, 米山勝美
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] いもち病菌感染イネのジベレリン分析2012

    • Author(s)
      真籠洋, 藤枝俊介, 花田篤志, 大里修一, 神谷勇治, 山口信次郎
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 赤かび病菌のトリコテセン系かび毒の後期生合成機構2012

    • Author(s)
      前田一行, 富永直樹, 兵藤壮一郎, 大里修一, 鎌倉高志, 米山勝美, 吉田稔, 木村真
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 赤かび病菌のトリコテセン系かび毒の後期生合成機構2012

    • Author(s)
      前田一行, 富永直樹, 兵藤壮一郎, 大里修一, 鎌倉高志, 米山勝美, 吉田稔, 木村真
    • Organizer
      日本農芸化学会2012年度
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 分泌性リパーゼの進化により生じるトリコテセン系かび毒の側鎖多様性-Tri104遺伝子の同定と側鎖脱アセチル化酵素の性質2012

    • Author(s)
      前田一行, 富永直樹, 兵藤壮一郎, 大里修一, 鎌倉高志, 米山勝美, 吉田稔, 木村真
    • Organizer
      日本マイコトキシン学会第70回学術講演会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2012-01-06
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イネいもち病菌における人為的DNA二本鎖切断誘導と相同組換え修復2011

    • Author(s)
      荒添貴之, 大里修一, 有江力, 米山勝美, 桑田茂
    • Organizer
      第11回糸状菌分子生物コンファレンス
    • Place of Presentation
      東京大学農学部,弥生講堂
    • Year and Date
      2011-11-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] GFP遺伝子を導入したカーブラリア葉枯病菌の侵入観察2011

    • Author(s)
      藤枝俊介, 大里修一, 有江力, 米山勝美
    • Organizer
      芝草学会,秋期大会
    • Place of Presentation
      信州大学農学部
    • Year and Date
      2011-10-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 相同組換えマーカー系を用いたイネいもち病菌の相同組換え機構の解析2011

    • Author(s)
      荒添貴之, 大里修一, 有江力, 米山勝美, 桑田茂
    • Organizer
      日本植物病理学会関東部会
    • Place of Presentation
      文部科学省研究交流センター2階国際会議場
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Identification of deacetylase genes that determine the side-chain modification patterns of trichothecenes in the late biosynthetic grid of type B trichothecenes2011

    • Author(s)
      K. Maeda, T. Tokai, M. Sato, S. Hyodo, N. Takahashi-Ando, S. Ohsato, M. Fujimura, T. Kamakura, K. Yoneyama, M. Yoshida, M. Kimura
    • Organizer
      International Union of Microbiological Societies 2011 Congress_XIII, International Congress of Mycology
    • Place of Presentation
      札幌,札幌コンベンションセンター/札幌市産業振興センター
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ゲノム再編誘発技術の開発に向けたイネ相同組換え検出系の構築2011

    • Author(s)
      杉崎彰, 大里修一, 佐久間美子, 近藤聡, 村本伸彦, 杉本広樹, 米山勝美, 光川典宏, 大音徳, 太田邦史
    • Organizer
      日本分子細胞生物学会(第29回)
    • Place of Presentation
      九州大学箱崎キャンパス
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ストレスおよび感染過程において誘導されるイネいもち病菌の体細胞相同組換えによる非病原性遺伝子(Avirulence gene)変異2011

    • Author(s)
      荒添貴之, 大里修一, 倉橋良雄, 有江力, 米山勝美
    • Organizer
      平成23年度日本植物病理学会大会
    • Place of Presentation
      東京農工大学府中キャンパス
    • Year and Date
      2011-03-29
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Curvularia属菌の侵入観察に向けたカーブラリア葉枯病菌の形質転換法の確立2011

    • Author(s)
      藤枝俊介, 大里修一, 有江力, 米山勝美
    • Organizer
      平成23年度日本植物病理学会大会
    • Place of Presentation
      東京農工大学府中キャンパス
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] イネいもち病菌における体細胞相同組換えの検出とその特色2010

    • Author(s)
      荒添貴之, 大里修一, 有江力, 倉橋良雄, 米山勝美
    • Organizer
      第10回糸状菌分子生物学コンファレンス
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2010-11-18
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 芝草病害のLAMP(loop-mediated isothermal amplification)法による簡易診断について2010

    • Author(s)
      永冨史子, 大里修一, 米山勝美
    • Organizer
      2010年度日本芝草学会春期大会
    • Place of Presentation
      千葉大学西千葉キャンパス
    • Year and Date
      2010-06-13
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] イネいもち病菌の相同組換え率に及ぼす化学ストレスの影響2010

    • Author(s)
      荒添貴之, 大里修一, 倉橋良雄, 有江力, 米山勝美
    • Organizer
      日本農薬学会第35回大会
    • Place of Presentation
      北海道大学学術交流会館
    • Year and Date
      2010-05-30
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 変異体植物、その製造方法及び遺伝的組換え頻度を上昇させる方法2011

    • Inventor(s)
      近藤聡,大音徳,太田邦史,大里修一,光川典宏,村本伸彦,杉本広樹
    • Industrial Property Number
      2010-007220
    • Filing Date
      2011-01-13
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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