Analysis of the origin and the mechanism of persistent infection of BmMLV
Project/Area Number |
22780045
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied entomology
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Research Institution | Utsunomiya University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | BmMLV / マキュラウイルス / 宿主域 / 持続感染 / 培養細胞 / バキュロウイルス発現系 / カイコ / バキュロウイルス / 感染性クローン / ウイルス / 遺伝子 / タンパク質 / 相互作用 |
Research Abstract |
To identify the origin of Bombyx mori macula-like virus, we analyzed the host range of BmMLV. Several insect-and mammalian-derived cell lines were inoculated with BmMLV and analyzed for the propagation of BmMLV. We showed that only B. mori-derived cell lines were permissive to BmMLV persistent infection. Also, BmMLV-negative BmVF cells were inoculated with BmNPV. We identified that the recombinant protein was fully expressed and suitable post-translational modification was occurred in BmNPV-infected BmVF cells. These data showed that BmMLV replicates only in B. mori-derived cells and BmVF cell line was suitable for baculovirus expression vector system.
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Report
(3 results)
Research Products
(14 results)