| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
To identify the origin of Bombyx mori macula-like virus, we analyzed the host range of BmMLV. Several insect-and mammalian-derived cell lines were inoculated with BmMLV and analyzed for the propagation of BmMLV. We showed that only B. mori-derived cell lines were permissive to BmMLV persistent infection. Also, BmMLV-negative BmVF cells were inoculated with BmNPV. We identified that the recombinant protein was fully expressed and suitable post-translational modification was occurred in BmNPV-infected BmVF cells. These data showed that BmMLV replicates only in B. mori-derived cells and BmVF cell line was suitable for baculovirus expression vector system.
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