Elucidation of genetic mechanisms underpinning a insect-microbe endosymbiosis.

• Project/Area Number: 22780051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KIKUCHI Yoshitomo 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30571864)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 応用動物 / 昆虫 / 微生物 / 細菌 / カメムシ / 共生細菌 / Burkholderia

Research Abstract

The aim of this study was to determine molecular mechanism as tohow Burkholderia symbiont specifically localizes in the symbiotic organ of the bean bug.Over 6,000 transposon-inserted mutants were investigated, revealing flagella motility of the symbiont p

Research Products

• [Journal Article] Efficient colonization of the bean bug Riptortus pedestris by an environmentally transmitted Burkholderiasymbiont (2013)
  • Author(s): Kikuchi, Y., et al.
  • Journal Title: Applied and Environmental Microbiology Volume: 79 Issue: 6 Pages: 2088-2091
  • DOI: 10.1128/aem.03299-12
  • Peer Reviewed
• [Journal Article] Polyphyly of gut symbionts in stinkbugs of the family Cydnidae (2012)
  • Author(s): Takahiro Hosokawa
  • Journal Title: Applied and Environmental Microbiology Volume: 78 Issue: 13 Pages: 4758-4761
  • DOI: 10.1128/aem.00867-12
  • Peer Reviewed
• [Journal Article] Novel clade of alphaproteobacterial endosymbionts associated with stink bugs and other arthropods. (2012)
  • Author(s): Matsuura Y., Kikuchi Y., Meng X. Y., Koga R., Fukatsu T.
  • Journal Title: Applied and Environmental Microbiology Volume: 78 Issue: 12 Pages: 4149-4156
  • DOI: 10.1128/aem.00673-12
  • Peer Reviewed
• [Journal Article] Gut symbiotic bacteria in the cabbage bugs Eurydema rugosa and Eurydema dominulus (Heteroptera : Pentatomidae) (2012)
  • Author(s): 菊池義智、細川貴弘, 他2名
  • Journal Title: Appl.Entomol.Zool. Volume: 47 Issue: 1 Pages: 1-8
  • DOI: 10.1007/s13355-011-0081-7
  • NAID: 40019166772
  • Peer Reviewed
• [Journal Article] Evolution of symbiotic organs and endosymbionts in lygaeid stinkbugs (2012)
  • Author(s): Y.Matsuura, Y.Kikuchi, T.Hosokawa, R.Koga, X.Y.Meng, Y.Kamagata, N.Nikoh, T.Fukatsu
  • Journal Title: The ISME Journal Volume: 6 Issue: 2 Pages: 397-409
  • DOI: 10.1038/ismej.2011.103
  • Peer Reviewed
• [Journal Article] Specific developmental window for establishment of an insect-microbe gut symbiosis (2011)
  • Author(s): Kikuchi, Y., et al.
  • Journal Title: Applied andEnvironmental Microbiology Volume: 77 Issue: 12 Pages: 4075-4081
  • DOI: 10.1128/aem.00358-11
  • Peer Reviewed
• [Journal Article] Symbiont-mediated insecticide resistance (2011)
  • Author(s): Kikuchi, Y., et al.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109 Issue: 22 Pages: 8618-8622
  • DOI: 10.1073/pnas.1200231109
  • Peer Reviewed
• [Journal Article] Obligate association with gut bacterial symbiont in Japanese populations of the southern green stinkbug Nezara viridula (Heteroptera : Pentatomidae) (2011)
  • Author(s): 多田明世、菊池義智, 他4名
  • Journal Title: Appl.Entomol.Zool. Volume: 46 Issue: 4 Pages: 483-488
  • DOI: 10.1007/s13355-011-0066-6
  • NAID: 40019130174
  • Peer Reviewed
• [Journal Article] An ancient but promiscuous host-symbiont association between Burkholderia gut symbionts and their heteropteran hosts. (2011)
  • Author(s): 菊池義智、細川貴弘、深津武馬
  • Journal Title: The ISME Journal Volume: 5 Pages: 446-460
  • Peer Reviewed
• [Journal Article] Bacterial symbionts of the giant jewel stinkbug Eucorysses grandis (Hemiptera : Scutelleridae). (2011)
  • Author(s): 貝和菜穗美、細川貴弘、菊池義智, その他5名
  • Journal Title: Zoological Science Volume: 28 Pages: 169-174
  • NAID: 40018724180
  • Peer Reviewed
• [Journal Article] Specific developmental window for establishment of an insect-microbe gut symbiosis. (2011)
  • Author(s): 菊池義智、細川貴弘、深津武馬
  • Journal Title: Applied and Environmental Microbiology Volume: 77(掲載確定)
  • Peer Reviewed
• [Journal Article] Primary gut symbiont and secondary, Sodalis-allied symbiont in the scutellerid stinkbug Cantao ocellatu. (2010)
  • Author(s): 貝和菜穂美、細川貴弘、菊池義智, その他5名
  • Journal Title: Applied and Environmental Microbiology Volume: 76 Pages: 3486-3494
  • Peer Reviewed
• [Journal Article] Phylogenetic position and peculiar genetic traits of a midgut bacterial symbiont of the stinkbug Parastrachia japonensis. (2010)
  • Author(s): 細川貴弘、菊池義智、二河成男, その他3名
  • Journal Title: Applied and Environmental Microbiology Volume: 76 Pages: 4130-4135
  • Peer Reviewed
• [Journal Article] Laccase2 is required for cuticular pigmentation and hardening in stink bugs. (2010)
  • Author(s): 二橋亮、田中康次郎、松浦優、棚橋薫彦、菊池義智、深津武馬
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 41 Pages: 191-196
  • Peer Reviewed
• [Presentation] 共生細菌の感染がホソへリカメムシ消化管の形態変化を引き起こす (2013)
  • Author(s): 菊池義智、Lee Bok Luel、深津武馬
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学
  • Year and Date: 2013-03-28
• [Presentation] 共生細菌の鞭毛運動性がホソヘリカメムシ腸内への特異的感染に果たす役割 (2013)
  • Author(s): 大林翼
  • Organizer: 2012年度日本応用動物昆虫学会・日本昆虫学会共催北海道支部大会
  • Place of Presentation: 北海道大学総合博物館
• [Presentation] 共生細菌の感染がホソへリカメムシ消化管の形態変化を引き起こす (2013)
  • Author(s): 菊池義智
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学生物資源科学部
• [Presentation] 泳げ、Burkholderia!～ホソヘリカメムシ共生細菌は鞭毛を使って共生器官に辿り着く～ (2013)
  • Author(s): 大林翼
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学生物資源科学部
• [Presentation] カンシャコバネナガカメムシにおける内部共生系のキャラクタリーゼーション (2013)
  • Author(s): 会田学
  • Organizer: 第57回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学生物資源科学部
• [Presentation] Symbiotic gut bacteria confer insecticide resistance (2012)
  • Author(s): 菊池義智
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術大学
  • Year and Date: 2012-09-22
• [Presentation] The Dark Side of the Moon:内部共生の分子基盤 (2012)
  • Author(s): 菊池義智
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学(奈良県)
  • Year and Date: 2012-03-28
• [Presentation] Insecticide resistance by endosymbiotic bacteria (2012)
  • Author(s): Yoshitomo Kikuchi
  • Organizer: 7th International Symbiosis Society Congress
  • Place of Presentation: Auditorium Maximum of the Jagiellonian University (Krakow, Poland)
• [Presentation] Insecticide resistance governed by endosymbiontic bacteria (2012)
  • Author(s): Yoshitomo Kikuchi
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: Exco-Daegu Convention Center, Daegu (Korea)
• [Presentation] Symbiotic gut bacteria confer insecticide resistance (2012)
  • Author(s): Yoshitomo Kikuchi
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術大学（愛知）
• [Presentation] Bacterial flagellar motility plays a pivotal role in an insect-microbe gut symbiosis (2012)
  • Author(s): Tsubasa Ohbayashi
  • Organizer: 14th International Symposium on Microbial Ecology
  • Place of Presentation: Copenhagen（Denmark）
• [Presentation] Establishment of artificial symbiotic system between Escherichia coli and an insect (2012)
  • Author(s): Yoshiko Ishii
  • Organizer: 14th International Symposium on Microbial Ecology
  • Place of Presentation: Copenhagen（Denmark）
• [Presentation] ホソヘリカメムシにおけるBurkholderia共生細菌の腸内選別 (2011)
  • Author(s): 菊池義智、細川貴弘、深津武馬
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-10-08
• [Presentation] ホソヘリカメムシにおけるBurkholderia共生細菌の腸内選別 (2011)
  • Author(s): 菊池義智
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-10-08
• [Presentation] Possibility of symbiont-mediated insecticide resistance (2011)
  • Author(s): 菊池義智
  • Organizer: 6th International Symposium of Molecular Insect Science
  • Place of Presentation: NH Grand Krasnapolsky Hotel (Amsterdam Netherlands)(招待講演)
  • Year and Date: 2011-10-03
• [Presentation] 昆虫内部共生研究のための新しいモデルシステム:ホソヘリカメムシとBurkbolderia共生細菌 (2011)
  • Author(s): 菊池義智
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都府)(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] The winnowing in an insect-microbe symbiosis : Bean bug selectively takes up a Burkholderia symbiont through specific gut structure and morphogenesis (2011)
  • Author(s): 菊池義智
  • Organizer: SMBE2011
  • Place of Presentation: 京都大学(京都府)(招待講演)
  • Year and Date: 2011-07-27
• [Presentation] To V, or not to V:共生細菌をいかに子に伝えるか? (2011)
  • Author(s): 菊池義智
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 九州大学(箱崎)
  • Year and Date: 2011-03-28
• [Presentation] 共生細菌が誘導するホソヘリカメムシ消化管の形態変化 (2011)
  • Author(s): 菊池義智、深津武馬
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 九州大学(箱崎)
  • Year and Date: 2011-03-27
• [Presentation] Amazing diversity of bacterial symbiosis in leeches. (2010)
  • Author(s): 菊池義智
  • Organizer: Memorial Symposium for the 26th International Prize for Biology
  • Place of Presentation: Tsukuba International Congress Center (Japan)
  • Year and Date: 2010-12-08
• [Presentation] Evolution of the symbiotic association between Burkholderia gut symbiont and stinkbugs (2010)
  • Author(s): 菊池義智、 細川貴弘、 深津武馬
  • Organizer: 3rd Conference on Beneficial Microbes
  • Place of Presentation: Hyatt Regency Miami(USA, FL)
  • Year and Date: 2010-10-25
• [Presentation] Evolution of the symbiotic association between Burkholderia gut sym biont and stinkbugs. (2010)
  • Author(s): 菊池義智、細川貴弘、深津武馬
  • Organizer: 3rd Conference on Beneficial Microbes
  • Place of Presentation: Hyatt Regency Miami (USA, FL)
  • Year and Date: 2010-10-25
• [Book] シリーズ現代の生態学11『微生物の生態学』、第8章(p.117-133) (2011)
  • Author(s): 菊池義智
  • Total Pages: 280
  • Publisher: 共立出版
• [Book] 微生物の生態学(シリーズ現代の生態学11)「第8 章 昆虫の共生微生物:その多様性と生態」
  • Author(s): 菊池義智(編)大園享司・鏡味麻衣子
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