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Identification of mechanism of chemoreception for host recognition and feeding habit evolution in a swallowtail butterflies

Research Project

Project/Area Number 22780052
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied entomology
Research InstitutionJT Biohistory Research Hall

Principal Investigator

OZAKI Katsuhisa  株式会社生命誌研究館, 研究員 (60396223)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsアゲハチョウ / 味覚受容体 / 化学感覚 / 生物間相互作用 / 食性進化 / 産卵行動 / RNA-seq / 食草選択 / GPCR / 7回膜貫通型受容体 / 7回膜貫通型タンパク質 / odorant binding protein / chemosensory protein
Research Abstract

Female of swallowtail butterflies select their host plant by tasting using chemo-sensory hair on their tarsi. I identified a mechanism of taste receptor for oviposition behavior. When expression of the receptor gene is interfered by RNAi method, butterflies show reduced sensitivity for the synephrine, one kind of 10 oviposition stimulants. This result suggests that this receptor gene affect to evolution driven by host-shift of phytophagous swallowtail butterflies.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (12 results) (of which Invited: 2 results) Book (4 results) Remarks (12 results)

  • [Journal Article] Gustatory sensing mechanism coding for multiple oviposition stimulants in the swallowtail butterfly2013

    • Author(s)
      Masasuke Ryuda, Delphine Calas-List, Ayumi Yamada, Frederic Marion-Poll, Hiroshi Yoshikawa, Teiichi Tanimura, and Katsuhisa Ozaki
    • Journal Title

      Papilio xuthus Journal of Neuroscience

      Volume: 33 (3) Issue: 3 Pages: 914-924

    • DOI

      10.1523/jneurosci.1405-12.2013

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ナミアゲハの産卵刺激物質を認識する味覚受容体2013

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Journal Title

      化学と生物

      Volume: Vol. 51 No. 3 Pages: 141-146

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アゲハチョウが植物を的確に見分けるしくみ2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Journal Title

      AROMA RESEARCH

      Volume: 50 Pages: 132-136

    • NAID

      40019340342

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アゲハチョウの化学センサー2012

    • Author(s)
      尾崎克久
    • Journal Title

      月刊化学2012年3月号

      Volume: 67 Pages: 32-37

    • Related Report
      2011 Annual Research Report
  • [Journal Article] A gustatory receptor involved in host plant recognition for oviposition of a swallowtail butterfly2011

    • Author(s)
      Katsuhisa Ozaki, Masasuke Ryuda, Ayumi Yamada, Ai Utoguchi, Hiroshi Ishimoto, Delphine Calas, Frederic Marion-Poll, Teiichi Tanimura & Hiroshi Yoshikawa
    • Journal Title

      Nature Communications

      Volume: 2:542 Issue: 1 Pages: 542-542

    • DOI

      10.1038/ncomms1548

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori2011

    • Author(s)
      Yoshizawa, Y., et al
    • Journal Title

      Insect Biochem Mol Biol

      Volume: 41 Pages: 545-562

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference in Lepidoptera : an overview of successful and unsuccessful studies and implications for experimental design2011

    • Author(s)
      Terenius, O., et al
    • Journal Title

      J Insect Physiol

      Volume: 57 Pages: 231-245

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アゲハチョウの産卵刺激物質受容体と食草選択2011

    • Author(s)
      尾崎克久
    • Journal Title

      昆虫と自然:特集

      Volume: 46(11) Pages: 10-14

    • Related Report
      2011 Annual Research Report
  • [Journal Article] アゲハチョウ産卵刺激物質受容体遺伝子の発見と機能解析2011

    • Author(s)
      尾崎克久、龍田勝輔、吉川寛
    • Journal Title

      昆虫DNA研究会ニュースレター

      Volume: 14 Pages: 44-47

    • Related Report
      2010 Annual Research Report
  • [Presentation] 苦味物質によるナミアゲハ(Papilio xuthus)産卵阻害効果2013

    • Author(s)
      龍田勝輔・尾崎克久
    • Organizer
      日本応用動物昆虫学会 第57回大会 2013. 3. 27-29
    • Place of Presentation
      日本大学湘南キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] アゲハチョウの産卵行動のための食草認識に関与する味覚受容体2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      (九州大学医学部: 福岡県福岡市)(招待講演)
    • Related Report
      2012 Final Research Report
  • [Presentation] アゲハチョウが産卵のために食草を認識する仕組みに関与する味覚受容体2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場: 福岡県福岡市(ワークショップ・ポスター)
    • Related Report
      2012 Final Research Report
  • [Presentation] アゲハチョウから新規化学受容体遺伝子をみつけたい2012

    • Author(s)
      尾崎 克久
    • Organizer
      NGS現場の会 第二回研究会 2012.5.24-25
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2012 Annual Research Report
  • [Presentation] アゲハチョウの産卵行動のための食草認識に関与する味覚受容体2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      九州大学医学部
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] アゲハチョウが産卵のために食草を認識する仕組みに関与する味覚受容体2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Organizer
      第35回日本分子生物学会年会(ワークショップ・ポスター)
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] A gustatory receptor involved in host-plant recognition for oviposition of the butterfly, Papilio xuthus.2011

    • Author(s)
      Ozaki, Katsuhisa, Ryuda, Masasuke and Yoshikawa, Hiroshi
    • Organizer
      The 59th Annual Meeting of the Entomological Society of America.
    • Place of Presentation
      アメリカ合衆国ネバダ州リノ市: シンポジウム招待講演
    • Year and Date
      2011-11-16
    • Related Report
      2012 Final Research Report
  • [Presentation] A gustatory receptor involved in host-plant recognition for oviposition of the butterfly, Papilio xuthus2011

    • Author(s)
      Ozaki, Katsuhisa
    • Organizer
      The 59^<th> Annual Meeting of the Entomological Society of America
    • Place of Presentation
      アメリカ合衆国ネバダ州リノ市(シンポジウム)(招待講演)
    • Year and Date
      2011-11-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] アゲハチョウの食草認識に関与する味覚受容体2011

    • Author(s)
      尾崎克久, 他
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(ポスター)(口頭)
    • Related Report
      2011 Annual Research Report
  • [Presentation] アゲハチョウ産卵の産卵行動に関与する受容体遺伝子の機能解析2010

    • Author(s)
      尾崎克久、龍田勝輔、吉川寛
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] アゲハチョウ産卵刺激物質受容体遺伝子の発見と機能解析2010

    • Author(s)
      尾崎克久、龍田勝輔、吉川寛
    • Organizer
      昆虫DNA研究会 第7回研究集会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] アゲハチョウ産卵刺激物質受容体遺伝子の発見と機能解析

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Organizer
      日本農芸化学会関西支部例会(第475回講演会)
    • Place of Presentation
      大阪府立大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] 化学受容の科学第6章 昆虫ゲノムに見る化学感覚受容体遺伝子の多様化 東原和成編2012

    • Author(s)
      尾崎克
    • Publisher
      化学同人
    • Related Report
      2012 Final Research Report
  • [Book] アゲハチョウが植物を的確に見分けるしくみ. AROMA RESEARCH No.50 2012/5 月号2012

    • Author(s)
      尾崎克久・龍田勝輔・吉川寛
    • Related Report
      2012 Final Research Report
  • [Book] 化学受容の科学 第6章 昆虫ゲノムに見る化学感覚受容体遺伝子の多様化2012

    • Author(s)
      尾崎克久
    • Total Pages
      12
    • Publisher
      化学同人
    • Related Report
      2012 Annual Research Report
  • [Book] 化学受容の科学:第6章昆虫ゲノムに見る化学受容体遺伝子の多様化2012

    • Author(s)
      東原和成編
    • Total Pages
      251
    • Publisher
      化学同人
    • Related Report
      2011 Annual Research Report
  • [Remarks] (1) RKB 毎日放送ラジオ局(福岡県)・中西一清スタミナラジオ (2011.12.2)

    • URL

      http://www.brh.co.jp/seimeishi/journal/069/research_1.html

    • Related Report
      2012 Final Research Report
  • [Remarks] (2) NHK ニュース(関西地区)・おはよう関西 (2011.11.16)

    • Related Report
      2012 Final Research Report
  • [Remarks] (1) NHK(久保隆記者)

    • Related Report
      2012 Final Research Report
  • [Remarks] (2) 読売新聞(阿部健記者)

    • Related Report
      2012 Final Research Report
  • [Remarks] (3) 毎日新聞(須田桃子記者)

    • Related Report
      2012 Final Research Report
  • [Remarks] (1) 日本経済新聞(松田記者)

    • Related Report
      2012 Final Research Report
  • [Remarks] (2) 共同通信(中沢記者): 写真等の資料を提供

    • Related Report
      2012 Final Research Report
  • [Remarks] (3) 朝日新聞(東山記者): 写真等の資料を提供

    • Related Report
      2012 Final Research Report
  • [Remarks] (4) 産経新聞(秋山記者)

    • Related Report
      2012 Final Research Report
  • [Remarks] 味覚受容体遺伝子がむすぶ化合物と産卵行動

    • URL

      http://www.brh.co.jp/seimeishi/journal/069/research_1.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.brh.co.jp/seimeishi/journal/069/research_1.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.brh.co.jp/seimeishi/journal/050/research_11_2.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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