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Mechanisms of chloroplast degradation by two-different autophagic pathways in senescent leaves

Research Project

Project/Area Number 22780054
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

ISHIDA Hiroyuki  東北大学, 大学院・農学研究科, 准教授 (60312625)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords老化 / 植物 / オートファジー / 葉緑体 / 細胞死
Research Abstract

I studied membrane dynamics and related genes among chloroplast autophagy that occurs in senescent leaves. Results suggested that (i)unlike RCBs, chloroplasts are transferred into the vacuolar lumen by invagination of tonoplasts ; (ii)in addition to ATG4, ATG2, 5, 7, and 10 are required for the chloroplast autophagy.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (16 results) Book (2 results)

  • [Journal Article] The changes of leaf carbohydrate contents as a regulator of autophagic degradation of chloroplasts via Rubisco-containing bodies during leaf senescence2011

    • Author(s)
      Izumi, M., Ishida, H.
    • Journal Title

      Plant Signal Behav

      Volume: 6 Pages: 685-687

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi, M., Wada, S., Makino, A., Ishida, H.
    • Journal Title

      Plant Physiol

      Volume: 154 Pages: 1196-1209

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナ葉の炭素制限環境におけるRCB/オートファジー系の生理的意義について2012

    • Author(s)
      泉正範、牧野周、石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 葉の老化と葉緑体のオートファジー2011

    • Author(s)
      石田宏幸、吉本光希、和田信也、泉正範、小野祐樹、牧野周
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] オートファジーによる葉緑体タンパク質の分解2011

    • Author(s)
      石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-03-22
    • Related Report
      2011 Final Research Report
  • [Presentation] シロイヌナズナにおけるRBCS1Aおよび3Bの変異が葉のRubisco量に与える影響の解析2011

    • Author(s)
      泉正範、角田穂奈美、鈴木雄二、牧野周、石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-03-22
    • Related Report
      2011 Final Research Report
  • [Presentation] オートファジーによる葉緑体タンパク質の分解2011

    • Author(s)
      石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナにおけるKBCS1Aおよび3Bの変異が葉のRubisco量に与える影響の解析2011

    • Author(s)
      泉正範, 角田穂奈美, 鈴木雄二, 牧野周, 石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナにおけるRubisco-containing body (RCB)形成の栄養要因に対する応答の解析2010

    • Author(s)
      泉正範、牧野周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      北海道(札幌市)
    • Year and Date
      2010-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] シロイヌナズナにおけるRubisco-containing body (RCB)形成の栄養要因に対する応答の解析2010

    • Author(s)
      泉正範, 牧野周, 石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      北海道
    • Year and Date
      2010-09-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] A possible role for plastid stromules in the autophagic transfer of stromal proteins to the vacuole2010

    • Author(s)
      Ishida, H., Izumi, M., Yoshimoto, K., Hanson, M. R., Makino, A.
    • Organizer
      Gordon Research conference ' Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ, イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2011 Final Research Report
  • [Presentation] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi, M., Makino, A., Ishida, H.
    • Organizer
      Gordon Research conference ' Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ, イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2011 Final Research Report
  • [Presentation] The impact of autophagic degradation in chloroplasts and other organelles during leaf senescence of Arabidopsis2010

    • Author(s)
      Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A.
    • Organizer
      Gordon Research conference ' Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ, イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2011 Final Research Report
  • [Presentation] A possible role for plastid stromules in the autophagic transfer of stromal proteins to the vacuole2010

    • Author(s)
      Ishida, H., Izumi, M., Yoshimoto, K., Hanson, M.R., Makino, A.
    • Organizer
      Gordon Research conference 'Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ、イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi, M., Makino, A., Ishida, H.
    • Organizer
      Gordon Research conference 'Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ、イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] The impact of autophagic degradation in chloroplasts and other organelles during leaf senescence of Arabidopsis2010

    • Author(s)
      Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A.
    • Organizer
      Gordon Research conference 'Mitochondria and Chloroplasts'
    • Place of Presentation
      バルガ、イタリア
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] A key factor for the autophagy of chloroplasts ; the production of Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi M., Makino A., Ishida H.
    • Organizer
      The 21st International Conference on Arabidopsis Research
    • Place of Presentation
      横浜市
    • Year and Date
      2010-06-06
    • Related Report
      2011 Final Research Report
  • [Presentation] A key factor for the autophagy of chloroplasts ; the production of Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi M., Makino A., Ishida H.
    • Organizer
      The 21st International Conference on Arabidopsis Research
    • Place of Presentation
      横浜
    • Year and Date
      2010-06-06
    • Related Report
      2010 Annual Research Report
  • [Book] Rubisco turnover and nitrogen in a leaf. Nitorgen assimilation in plants. Research Sinpost2010

    • Author(s)
      Ishida, H., Suzuki, Y., Makino A.
    • Publisher
      Research Sinpost
    • Related Report
      2011 Final Research Report
  • [Book] Protein turnover in grass leaves. Advances in Botanical Research2010

    • Author(s)
      Irving, L. J., Suzuki, Y., Ishida, H., Makino, A., Kader, J. C. and Delseny, M. Eds., Elsevier
    • Publisher
      Elsevier
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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