Study of the root-knot nematode infection mechanism using a model tomato, Micro-Tom

• Project/Area Number: 22780056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: University of Tsukuba
• Principal Investigator: ASAMIZU Erika 筑波大学, 生命環境系, 准教授 (00370924)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 土壌生物 / ネコブセンチュウ / 菌根菌共生 / 園芸作物 / トマト / 共生シグナル伝達系 / マイクロトム / RNAi / 共生シグナル / エチレン

Research Abstract

To analyze the host plant response mechanism related to the parasitic root-knot nematode infection, we performed the infection assay using ethylene-insensitive mutant lines and knockdown lines of symbiosis genes, of a dwarf model tomato cv. Micro-Tom. The result indicated that the egg mass formation was suppressed in the ethylene-insensitive lines with the suppression level positively linked to the degree of insensitivity. It was also shown that the root-knot nematode infection was suppressed in the knockdown lines of symbiosis genes.

Research Products

• [Journal Article] Tomato TILLING technology : development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries (2011)
  • Author(s): Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Bres C, Rothan C, Mizoguchi T, Ezura H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 1994-2005
  • NAID: 10030305636
  • URL: http://hdl.handle.net/2241/114770
• [Presentation] Derek Goto, Erika Asamizu Potential involvement of common signaling pathway in the root-knot nematode infection process (2012)
  • Author(s): Narumi Souda, Shuhei Hayashi, Yoshihiro Okabe, Hiroshi Ezura, Masayoshi Kawaguchi
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Potential involvement of common signaling pathway in the root-knot nematode infection process (2012)
  • Author(s): Narumu Souda, Shuhei Hayashi, Yoshihiro Okabe, Hiroshi Ezura, Masayoshi Kawaguchi, Derek Goto, Erika Asamizu
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] Host infection by the root-knot nematode Meloidogyne hapla depends onparasitic utilisation of a common plant signaling pathway (2011)
  • Author(s): Shuhei Hayashi, Yosuke Maruyama, Tatsuhiro Ezawa, Mitsuru Osaki, Masayoshi Kawaguchi, Junji Yamaguchi, Erika Asamizu, Derek B. Goto
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Joint Conference
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2011-11-29
• [Presentation] Ethylene signaling pathway in interaction between sedentary nematode and tomato (2011)
  • Author(s): Yoshihiro Okabe, Narumi Souda, Hiroshi Ezura, Erika Asamizu
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Joint Conference
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2011-11-29
• [Presentation] Ethylene signaling pathway in interaction between sedentary nematode and tomato (2011)
  • Author(s): Yoshihiro Okabe, Narumi Souda, Hiroshi Ezura, Erika Asamizu
  • Organizer: 8^<th> Solanaceae and 2^<nd> Cucurbitaceae Joint Conference
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2011-11-29
• [Presentation] Host infection by the root-knot nematode Meloidogyne hapla depends on parasitic utilisation of a common plant signaling pathway (2011)
  • Author(s): Shuhei Hayashi, Yosuke Maruyama, Tatsuhiro Ezawa, Mitsuru Osaki, Masayoshi Kawaguchi, Junji Yamaguchi, Erika Asamizu, Derek B.Goto
  • Organizer: 8^<th> Solanaceae and 2^<nd> Cucurbitaceae Joint Conference
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2011-11-29
• [Presentation] Mutant analysis suggests root-knot nematode requires part of the symbiotic pathway for parasitic infection (2011)
  • Author(s): 林周平、江沢辰広、大崎満、浅水恵理香、川口正代司、後藤デレック
  • Organizer: 第21回植物微生物研究会交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21
• [Presentation] 矮性モデルトマト・マイクロトムのEMS変異誘発系統を用いたネコブセンチュウ感染性の解析 (2011)
  • Author(s): 岡部佳弘、江面浩、GOTO Derek、浅水恵理香
  • Organizer: 第21回植物微生物研究会交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21
• [Presentation] Potential role of regulatory genes in the root-knot nematode infection process (2011)
  • Author(s): 浅水恵理香、岡部佳弘、江面浩、Arshana Noorul Amin, Derek Goto
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Presentation] Potential role of regulatory genes in the root-knot nematode infection process (2011)
  • Author(s): 浅水恵理香、岡部佳弘、江面浩、Arshana Noorul Amin, Derek Goto
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: (東北大学※地震により中止されたが、発表は成立。)
  • Year and Date: 2011-03-20
• [Presentation] Potential role of regulatory genes in the root-knot nematode infection process (2011)
  • Author(s): 浅水恵理香、岡部佳弘、江面浩、奥村新、江沢辰広、Derek Goto
  • Organizer: 日本ナス科・ウリ科ゲノム合同国際シンポジウム
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-03-05
• [Patent(Industrial Property Rights)] 植物のネコブセンチュウ抵抗性の増加方法 (2011)
  • Inventor(s): 浅水恵理香,岡部佳弘
  • Industrial Property Rights Holder: 国立大学法人筑波大学
  • Industrial Property Number: 2011-191888
  • Filing Date: 2011-09-02
• [Patent(Industrial Property Rights)] 植物のネコブセンチュウ抵抗性の増加方法 (2011)
  • Inventor(s): 浅水恵理香、岡部佳弘
  • Industrial Property Rights Holder: 筑波大学
  • Industrial Property Number: 2011-191888
  • Filing Date: 2011-09-02