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An Exhaustive Screening and Functional Enhancement of a Useful Genes Related to Hydrogen Production by Using a Genetic Engineering Approach.

Research Project

Project/Area Number 22780070
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyushu Institute of Technology

Principal Investigator

MAEDA Toshinari  九州工業大学, 大学院・生命体工学研究科, 准教授 (00470592)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords水素ガス / バイオテクノロジー / 遺伝子工学 / 生体機能利用 / 代謝工学 / 水素 / 微生物 / 遺伝子機能解析 / 発酵
Research Abstract

The purpose of this study is to enhance bacterial hydrogen production. During this research activity, an exhaustive screening using an Escherichia coli mutant library was performed to find a useful gene related to hydrogen production, which could be helpful to fully understand biohydrogen production in E. coli; as a result, there are several uncharacterized genes, which have been never reported that they are related to hydrogen production. In addition, the function of E. coli hydrogenases in hydrogen fermentation from glycerol was studied.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (18 results) Book (3 results) Remarks (7 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Influence of Escherichia colihydrogenases on hydrogen fermentation from glycerol2013

    • Author(s)
      V. Sanchez-Torres, M. Z. Mohd Yusoff, C.Nakano, T. Maeda, H.I. Ogawa, T. K. Wood
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: Vol.38, No.10 Pages: 3905-3912

    • URL

      http://dx.doi.org/10.1016/j.ijhydene.2013.01.031

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of Escherichia coli hydrogenases on hydrogen fermentation from glycerol2013

    • Author(s)
      Viviana Sanchez-Torres
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: 38 Issue: 10 Pages: 3905-3912

    • DOI

      10.1016/j.ijhydene.2013.01.031

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production2012

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, V. Sanchez-Torres, H. I. Ogawa, Y. Shirai, T. K. Wood
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: Vol.37, No.23 Pages: 17778-17787

    • URL

      http://dx.doi.org/10.1016/j.ijhydene.2012.08.115

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS12012

    • Author(s)
      S. N. Suhaimi, L.-Y. Phang, T. Maeda,S. Abd-Aziz, M. Wakisaka, Y. Shirai, M. A. Hassan
    • Journal Title

      Brazilian Journal of Microbiology

      Volume: Vol.43, No.2 Pages: 506-516

    • NAID

      120006452530

    • URL

      http://dx.doi.org/10.1590/S1517-83822012000200011

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen production by recombinant Escherichia coli strains2012

    • Author(s)
      T. Maeda, V. Sanchez-Torres, T. K. Wood
    • Journal Title

      Microbial Biotechnology

      Volume: Vol.5, No.2 Pages: 214-225

    • URL

      http://dx.doi.org/10.1111/j.1751-7915.2011.00282.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production2012

    • Author(s)
      Mohd Zulkhairi Mohd Yusoff
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: 37 Issue: 23 Pages: 17778-17787

    • DOI

      10.1016/j.ijhydene.2012.08.115

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS12012

    • Author(s)
      Sheril Noriana Suhaimi
    • Journal Title

      Brazilian Journal of Microbiology

      Volume: 43 Issue: 2 Pages: 506-516

    • DOI

      10.1590/s1517-83822012000200011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen production by recombinant Escherichia coli strains2012

    • Author(s)
      Toshinari Maeda, Viviana Sanchez-Torres, Thomas K.Wood
    • Journal Title

      Microbial Biotechnology

      Volume: 5 Issue: 2 Pages: 214-225

    • DOI

      10.1111/j.1751-7915.2011.00282.x

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quorum quenching quandary : resistance to antivirulence compounds2012

    • Author(s)
      Toshinari Maeda, Rodolfo Garcia-Conrtreas, Mingming Pu, Lili Sheng, Luis Rene Garcia, Maria Tomas, Thomas K.Wood
    • Journal Title

      The ISME Journal

      Volume: 6 Issue: 3 Pages: 493-501

    • DOI

      10.1038/ismej.2011.122

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高度水素生産菌株を活用した迅速な安全性評価技術の開発2011

    • Author(s)
      前田憲成、 有吉弘貴、 高柳聖、 尾川博昭
    • Journal Title

      用水と廃水

      Volume: 53巻 Pages: 458-464

    • NAID

      40018862436

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of a serine protease secreted by Brevibacillus sp.KH3 for reducing waste activated sludge and biofilm formation2011

    • Author(s)
      Toshinari Maeda
    • Journal Title

      Bioresource Technology

      Volume: VOL.102 Issue: 22 Pages: 10650-10656

    • DOI

      10.1016/j.biortech.2011.08.098

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高度水素生産菌株を活用した迅速な安全性評価技術の開発2011

    • Author(s)
      前田憲成, 有吉弘貴, 高柳聖, 尾川博昭
    • Journal Title

      用水と廃水

      Volume: 53 Pages: 458-464

    • NAID

      40018862436

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global regulator H-NS and lipoprotein NlpI influence production of extracellular DNA in Escherichia coli2010

    • Author(s)
      Sanchez-Torres Viviana
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: Vol.401 Pages: 197-202

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Biohydrogen production from sewage sludge and oil palm frond (OPF) juice by a metabolically engineered Escherichia coli strain2013

    • Author(s)
      N. H. Mohd Yasin, T. Maeda, M. Fukuzaki, T. Miyazaki, C. M. H. Che Maail, H.Ariffin
    • Organizer
      平成 24 年度日本水環境学会九州支部大会
    • Place of Presentation
      北九州産業学術推進機構(北九州)
    • Year and Date
      2013-02-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Biohydrogen production from sewage sludge and oil palm frond (OPF) juice by a metabolically engineered Escherichia coli strain2013

    • Author(s)
      Nazlina Haiza Mohd Yasin
    • Organizer
      平成24年度日本水環境学会九州支部大会
    • Place of Presentation
      北九州産業学術推進機構
    • Related Report
      2012 Annual Research Report
  • [Presentation] Unrealized function of Escherichia coli genes; sufD and yehP in biohydrogen production2012

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, Y.Hashiguchi, T. K. Wood, Y. Shirai, H. I. Ogawa, M. A. Hassan
    • Organizer
      創立 90 周年記念第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] グリセロールからの水素発酵における大腸菌ヒドロゲナーゼの機能解析2012

    • Author(s)
      中野智恵理、前田憲成、尾川博昭、V.Sanchez-Torres
    • Organizer
      第46回日本水環境学会年会
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2012-03-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝子工学的組換え微生物による下水余剰汚泥の可溶化成分からの水素発酵2012

    • Author(s)
      福崎雅治、野中智徳、前田憲成、尾川博昭
    • Organizer
      第46回日本水環境学会年会
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2012-03-14
    • Related Report
      2012 Final Research Report
  • [Presentation] グリセロールからの水素発酵における大腸菌ヒドロゲナーゼの機能解析2012

    • Author(s)
      中野智恵理, 前田憲成, 尾川博昭, Viviana Sanchez-Torres
    • Organizer
      第46回日本水環境学会年会
    • Place of Presentation
      東洋大学(東京都)
    • Year and Date
      2012-03-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Unrealized function of Escherichia coli genes; sufD and yehP in biohydrogen production2012

    • Author(s)
      Mohd Zulkhairi Mohd Yusoff
    • Organizer
      創立90周年記念第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional analysis of an important gene related to biohydrogen production in Escherichia coli, BioMicroWorld 2011, IV International Conference on Environmental2011

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, V. SanchezTorres, T. K. Wood, Y. Shirai, H. I. Ogawa, M. A. Hassan
    • Organizer
      Industrial and Applied Microbiology
    • Place of Presentation
      Malaga (Spain)
    • Year and Date
      2011-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional analysis of an important gene related to biohydrogen production in Escherichia coli2011

    • Author(s)
      Mohd Zulkhairi Mohd Yusoff, Toshinari Maeda, Viviana Sanchez-Torres, Thomas K.Wood, Yoshihito Shirai, Hiroaki I.Ogawa, Mohd Ali Hassan
    • Organizer
      BioMicroWorld 2011, IV International Conference on Environmental, Industrial and Applied Microbiology
    • Place of Presentation
      Torremolinos Conference Room (Malaga, Spain)
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高度水素生産菌株を活用した簡易・迅速な毒性検出技術の開発2011

    • Author(s)
      有吉弘貴, 前田憲成, V. Sanchez-Torres, 尾川博昭
    • Organizer
      環境バイオテクノロジー学会 2011 年度大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-06-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌ヒドロゲナーゼ変異株によるグリセロールからの水素発酵2011

    • Author(s)
      前田憲成 , 中野智恵理 , V. SanchezTorres, M. Z. Mohd Yusoff, 尾川博昭
    • Organizer
      環境バイオテクノロジー学会 2011 年度大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-06-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌ヒドロゲナーゼ変異株によるグリセロールからの水素発酵2011

    • Author(s)
      前田憲成, 中野智恵理, SANCHEZ-TORRES Viviana, MOHD YUSOFF Mohd Zulkhairi, 尾川博昭
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of pili in S.sanguinis to the cell adhesion2010

    • Author(s)
      Saeki Ryosuke
    • Organizer
      The 58th Annual Meeting of Japanese Association for Dental Research (JADR2010)
    • Place of Presentation
      九州歯科大学(北九州市)
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Exhaustive Screening of 3985 Isogenic Escherichia coli Mutants for Enhanced Bacterial Hydrogen Production2010

    • Author(s)
      T. Maeda,V. Sanchez-Torres,T. K. Wood
    • Organizer
      2010 American Institute of Chemical Engineers (AIChE) Annual Meeting
    • Place of Presentation
      Salt Lake City (USA)
    • Year and Date
      2010-11-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Exhaustive screening of 3985 isogenic Escherichia coli mutants for enhanced bacterial hydrogen production2010

    • Author(s)
      Maeda Toshinari
    • Organizer
      2010 American Institute of Chemical Engineers(AIChE)Annual Meeting
    • Place of Presentation
      Salt Palace Convention Center(米国、ソルトレイクシティ)
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Biological treatment of harmful TNT wastewater containing a high concentration of nitrogen compounds by waste activated sludge2010

    • Author(s)
      Saeki Ryosuke
    • Organizer
      14th International Biotechnology Symposium and Exhibition (IBS2010)
    • Place of Presentation
      Palacongressi(イタリア、リミニ)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Behaviour of DNA components during thermophilic aerobic digestion of waste activated sludge by Brevibacillus sp.KH32010

    • Author(s)
      Mouri Kentaro
    • Organizer
      14th International Biotechnology Symposium and Exhibition (IBS2010)
    • Place of Presentation
      Palacongressi(イタリア、リミニ)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 下水汚泥溶解細菌による余剰汚泥の高温処理におけるDNAの挙動2010

    • Author(s)
      毛利謙太郎
    • Organizer
      環境バイオテクノロジー学会2010年度大会
    • Place of Presentation
      東北大学片平さくらホール(仙台市)
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Book] 九工大世界トップ技術2011

    • Author(s)
      前田憲成, 中野光一, 尾川博昭
    • Publisher
      西日本新聞社
    • Related Report
      2012 Final Research Report
  • [Book] Microbial Degradation of Xenobiotics2011

    • Author(s)
      T. Maeda, H. I. Ogawa
    • Publisher
      Springer
    • Related Report
      2012 Final Research Report
  • [Book] Microbial Degradation of Xenobiotics2011

    • Author(s)
      Toshinari Maeda, Hiroaki I.Ogawa
    • Total Pages
      26
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Remarks] (1)大学ホームページにおける研究代表者のデータベース

    • URL

      https://research02.jimu.kyutech.ac.jp/html/333_ja.html

    • Related Report
      2012 Final Research Report
  • [Remarks] (2)研究室のホームページ

    • URL

      http://www.life.kyutech.ac.jp/~toshi.maeda/

    • Related Report
      2012 Final Research Report
  • [Remarks] 大学ホームページにおける研究者のデーターベース

    • URL

      https://research02.jimu.kyutech.ac.jp/html/333_ja.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 研究室のホームページ

    • URL

      http://www.life.kyutech.ac.jp/~toshi.maeda/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.life.kyutech.ac.jp/~toshi.maeda/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://horyu.jimut.kyutech.ac.jp/kyouin/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://horyu.jimut.kyutech.ac.jp/kyouin/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 被験物のスクリーニング方法2010

    • Inventor(s)
      前田憲成、尾川博昭
    • Industrial Property Rights Holder
      九州工業大学
    • Industrial Property Number
      2010-056824
    • Filing Date
      2010-03-13
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 被験物のスクリーニング方法2010

    • Inventor(s)
      前田憲成
    • Industrial Property Rights Holder
      九州工業大学
    • Industrial Property Number
      2010-056824
    • Filing Date
      2010-03-13
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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