What establishes the symbiotic relationship between human and bifidobacteria?"A bifidogenic effects of human milk oligosaccharides"

• Project/Area Number: 22780072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: KATAYAMA Takane 石川県立大学, 生物資源環境学部, 准教授 (70346104)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ヒト母乳オリゴ糖 / ビフィズス菌 / 共生 / グリコシダーゼ / 母乳オリゴ糖 / ビフィズス因子 / オリゴ糖代謝 / 腸内細菌 / ミルクオリゴ糖 / ムチン型糖鎖 / 精密オリゴ糖合成

Research Abstract

The breast-fed infant intestine is often colonized by particular bifidobacteria, and human milk oligosaccharides(HMOs) are considered to be bifidogenic ; however, the precise mechanism underlying it remains unresolved. We have isolated and characterized various glycosidases that act on HMOs from Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. longum and revealed how these enzymes are involved in the degradation of HMOs by these bifidobacteria. These results provide us with essential and basic understanding of physiology of bifidogenic effects of HMOs, and suggested co-evolution of bifidobacteria with humans. We also developed an efficient method of enzymatic synthesis of 2'-fucosyllactose, the most abundant component of HMOs, by introducing a glycosynthase technology into 1, 2-α-L-fucosidase.

Research Products

• [Journal Article] Bifidobacterium longum subsp. infantis uses two differentβ-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides (2012)
  • Author(s): Yoshida E, Sakurama H, Kiyohara M, Nakajima M, Kitaoka M, Ashida H, Hirose J, Katayama T, Yamamoto K, Kumagai H.
  • Journal Title: Glycobiology Volume: 22 Pages: 361-368
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Journal Article] α-N-Acetylgalactosaminidase from infant-associated bifidobacteria belonging to novel glycoside hydrolase family 129 is implicated in alternative mucin degradation pathway (2012)
  • Author(s): Kiyohara M, Nakatomi T, Kurihara S, Fushinobu S, Suzuki H, Tanaka T, Shoda S, Kitaoka M, Katayama T, Yamamoto K, Ashida H
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 693-670
  • Peer Reviewed
• [Journal Article] Bifidobacterium longum subsp.infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides (2012)
  • Author(s): Yoshida E, Sakurama H, Kiyohara M, Nakajima M, Kitaoka M, Ashida H, Hirose J, Katayama T, Yamamoto K, Kumagai H
  • Journal Title: Glycobiology Volume: 22 Pages: 361-368
  • Peer Reviewed
• [Journal Article] Physiology of the consumption of human milk oligosaccharides by infant-gut associated bifidobacteria (2011)
  • Author(s): Asakuma S, Hatakeyama E, Urashima T, Yoshida E, Katayama T, Yamamoto K, Kumagai H, Ashida H, Hirose J, Kitaoka M.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 34583-34592
  • URL: http://www.jbc.org/
  • Peer Reviewed
• [Journal Article] An exo-α-sialidase from bifidobacteria involved in the degradation of sialyloligosaccharides in human milk and intestinal glycoconjugates (2011)
  • Author(s): Kiyohara M, Tanigawa K, Chaiwangsri T, Katayama T, Ashida H, Yamamoto K.
  • Journal Title: Glycobiology Volume: 21 Pages: 437-447
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Journal Article] Crystal structures of phosphoketolase : thiamine diphosphate-dependent dehydration mechanism (2010)
  • Author(s): Suzuki R, Katayama T, Kim BJ, Wakagi T, Shoun H, Ashida H, Yamamoto K, and Fushinobu S.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 34279-34287
  • URL: http://www.jbc.org/
  • Peer Reviewed
• [Journal Article] Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve (2010)
  • Author(s): Suzuki R, Kim BJ, Shibata T, Iwamoto Y, Katayama T, Ashida H, Wakagi T, Shoun H, Fushinobu S, and Yamamoto K.
  • Journal Title: Acta Crystallographica Section F. Structural Biology and Crystallization Communications Volume: 66 Pages: 941-943
  • URL: http://journals.iucr.org/f/
  • Peer Reviewed
• [Journal Article] Cooperation ofβ-galactosidase andβ-N-acetyl-hexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure (2010)
  • Author(s): Miwa M, Horimoto T, Kiyohara M, Katayama T, Kitaoka M, Ashida H, and Yamamoto K.
  • Journal Title: Glycobiology Volume: 20 Pages: 1402-1409
  • URL: http://glycob.oxfordjournals.org/
  • Peer Reviewed
• [Journal Article] Crystal structures of phosphoketolase : thiamine diphosphate-dependent dehydration mechanism (2010)
  • Author(s): Suzuki, R., et al.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 34279-34284
  • Peer Reviewed
• [Journal Article] Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve (2010)
  • Author(s): Suzuki, R., et al.
  • Journal Title: Acta Crystallographica Section F.Structural Biology and Crystallization Communications Volume: 66 Pages: 941-943
  • Peer Reviewed
• [Journal Article] Cooperation of b-galactosidase and b-N-acetylhexosaminidase from bifidobacteria in assimilation of human milk oligosaccharides with type 2 structure (2010)
  • Author(s): Miwa, M., et al.
  • Journal Title: Glycobiology Volume: 20 Pages: 1402-1499
  • Peer Reviewed
• [Presentation] ビフィズス菌の共生戦略 (2012)
  • Author(s): 片山高嶺, 芦田久, 熊谷英彦, 山本憲二
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-25
• [Presentation] 1,3-1,4-α-L-フコシダーゼの結晶構造解析に基づく基質特異性の構造学的基盤 (2012)
  • Author(s): 楼間晴子, 伏信進矢, 北岡本光, 日高詩文, 芦田久, 片山高嶺, 山本憲二, 熊谷英彦
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] Bifidobacterium bifidum由来の1,3-1,4-α-L-フコシダーゼの改変によるルイスa/x抗原の合成 (2011)
  • Author(s): 桜間晴子, 吉田永史奈, 本多裕司, 芦田久, 北岡本光, 片山高嶺, 山本憲二, 熊谷英彦
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-09-28
• [Presentation] ビフィズス菌のヒトミルクオリゴ糖代謝経路 (2011)
  • Author(s): 北岡本光, 西本完, 片山高嶺, 清原正志, 和田潤, 芦田久, 山本憲二, 浦島匡
  • Organizer: 63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] ビフィズス菌によるヒトミルクオリゴ糖の分解-培養上清におけるオリゴ糖濃度の経時変化- (2011)
  • Author(s): 片山高嶺, 朝隈貞樹, 畑山恵美, 浦島匡, 吉田永史奈, 熊谷英彦, 芦田久, 廣瀬潤子, 北岡本光, 山本憲二
  • Organizer: 63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] A UNIQUE PATHWAY OF BIFIDOBACTERIA FOR THE DEGRADATION OF HUMAN MILK OLIGOSACCHARIDES (2011)
  • Author(s): Takane Katayama, Hisashi Ashida, Motomitsu Kitaoka, Shinya Fushinobu, Hidehiko Kumagai, and Kenji Yamamoto
  • Organizer: International Union of Microbiological Societies 2011Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-09
• [Presentation] A UNIQUE PATHWAY OF BIFIDOBACTERIA FOR THE DEG RADATION OF HUMAN MILK OLIGOSACCHARIDES (2011)
  • Author(s): Katayama T, Ashida H, Kitaoka M, Fushinobu S, Kumagai H, Yamamoto K
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-09
• [Presentation] ビフィズス菌ヒトミルクオリゴ糖分解酵素を利用したフコシルオリゴ糖の調製 (2011)
  • Author(s): 片山高嶺, 本多裕司, 熊谷英彦, 山本憲二
  • Organizer: 日本農芸化学会2011年度大会シンポジウム
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-28
• [Presentation] Bifidobacterium longum subsp. infantis ATCC15697株のヒトミルクオリゴ糖分解酵素 (2011)
  • Author(s): 吉田永吏奈, 中島将博, 北岡本光, 廣瀬潤子, 清原正志, 片山高嶺, 山本憲二, 熊谷英彦
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] 1,3-1,4-a-L-フコシンターゼによるルイスa/x抗原の合成 (2011)
  • Author(s): 櫻間晴子, ら
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-26
• [Presentation] Conversion of 1,2-a-L-fucosidase to 1,2-a-L-fucosynthas (2010)
  • Author(s): Katayama, T., et al.
  • Organizer: The 12^<th> Japanese-Swiss Meeting on Biotechnology and Bioprocess Development
  • Place of Presentation: Toyama
  • Year and Date: 2010-10-11
• [Book] Lactic Acid Bacteria and bifidobacteria : Current Progress in Advanced Research (2011)
  • Author(s): Kitaoka M, Katayama T, Yamamoto K
  • Total Pages: 286
  • Publisher: Metabolic pathway of human milk oligosaccharides in bifidobacteira (Chapter 3). pp53-65
• [Book] 乳酸菌とビフィズス菌のサイエンス(ヒトミルクオリゴ糖の代謝) (2010)
  • Author(s): 北岡本光, ら
  • Total Pages: 668
  • Publisher: 京都大学学術出版会