| Project/Area Number |
22780076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
TAKAKU Hiroaki 新潟薬科大学, 応用生命科学部, 准教授 (70350717)
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| Research Collaborator |
NASHIMOTO Masayuki 新潟薬科大学, 応用生命科学部, 教授 (30228069)
TAKAGI Masamichi 新潟薬科大学, 学長 (50018339)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 微生物酵素 / tRNaseZL / 前駆体tRNA / 酵母 / tRNAの3'端プロセシング / endoribonuclease |
|---|
| Research Abstract |
Endoribonuclease tRNase Z catalyzes the generation of the mature 3' end of tRNA precursors through specific endonucleolytic cleavage at discriminator site in eukaryotic organisms. The budding yeast Saccharomyces cerevisiae has a single long form of tRNase Z. The recombinant yeast tRNase Z protein possesses precursor tRNA 3'-processing activity in vitro. The yeast tRNase Z protein localizes to the nucleus and is essential for growth. Temperature-sensitive(Ts) mutants of yeast tRNase Z exhibit the accumulation of 3′-unprocessed pre-tRNAs. Thus, our findings suggest that the sctRNase ZL may be required for the 3′end processing of pre-tRNAs.
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