Search, identification and investigation of the useful mutations for improvement of the function for secretion or metabolism in Bacillus subtilis
| Project/Area Number |
22780078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
INAOKA Takashi 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノロジー研究領域, 主任研究員 (40391205)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | スカンジウム / ウンデカプレニル2リン酸合成酵素 / アミラーゼ / グルコース6リ ン酸デヒドロゲナーゼ / NADPH / メタボローム / ペントースリン酸経路 / グルコース６リン酸デヒドロゲナーゼ遺伝子 / ネオトレハロサジアミン / 枯草菌 / バシトラシン |
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| Research Abstract |
In B. subtilis, a undecaprenyl pyrophosphate synthase mutation was found to give rise to increased resistance to both a rare earth element scandium and bacitracin, which prevents cell wall synthesis, in addition to enhanced amylase production. Transcriptional analysis revealed no significant difference in the amylase gene expression, indicating that the effect of this mutation on amylase overproduction was exerted at the posttranscriptional level. This mutation thus has a potential to stimulate the amylase secretion.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production
Author(s)
Kubo, Y., Inaoka, T., Hachiya, T., Miyake, M., Hase, S., Nakagawa, R., Hasegawa, H., Funane, K., Sakakibara, Y., and Kimura, K
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Journal Title
J.Biosci.Bioeng
Volume: (In press)
NAID
Related Report
Peer Reviewed
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