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Search, identification and investigation of the useful mutations for improvement of the function for secretion or metabolism in Bacillus subtilis

Research Project

Project/Area Number 22780078
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

INAOKA Takashi  独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノロジー研究領域, 主任研究員 (40391205)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsスカンジウム / ウンデカプレニル2リン酸合成酵素 / アミラーゼ / グルコース6リ ン酸デヒドロゲナーゼ / NADPH / メタボローム / ペントースリン酸経路 / グルコース6リン酸デヒドロゲナーゼ遺伝子 / ネオトレハロサジアミン / 枯草菌 / バシトラシン
Research Abstract

In B. subtilis, a undecaprenyl pyrophosphate synthase mutation was found to give rise to increased resistance to both a rare earth element scandium and bacitracin, which prevents cell wall synthesis, in addition to enhanced amylase production. Transcriptional analysis revealed no significant difference in the amylase gene expression, indicating that the effect of this mutation on amylase overproduction was exerted at the posttranscriptional level. This mutation thus has a potential to stimulate the amylase secretion.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (5 results)

  • [Journal Article] Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production.2013

    • Author(s)
      Yuji Kubo
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 115 Issue: 6 Pages: 654-657

    • DOI

      10.1016/j.jbiosc.2012.12.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements2012

    • Author(s)
      Inaoka, T., and Ochi, K
    • Journal Title

      J.Bacteriol

      Volume: 194 Pages: 5632-5637

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements2012

    • Author(s)
      Takashi Inaoka
    • Journal Title

      Journal of Bacteriology

      Volume: 194 Issue: 20 Pages: 5632-5637

    • DOI

      10.1128/jb.01147-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activation of dormant secondary metabolism neotrehalosadiamine synthesis by RNA polymerase mutation in Bacillus subtilis2011

    • Author(s)
      Inaoka, T., and Ochi, K
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 618-623

    • NAID

      10028271088

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis2011

    • Author(s)
      Inaoka, T., and Ochi, K
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 77 Pages: 8181-8183

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis2011

    • Author(s)
      Inaoka T, Ochi K
    • Journal Title

      Appl Environ Microbiol

      Volume: 77 Issue: 22 Pages: 8181-8183

    • DOI

      10.1128/aem.06205-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activation of Dormant Secondary Metabolism Neotrehalosadiamine Synthesis by an RNA Polymerase Mutation in Bacillus subtilis2011

    • Author(s)
      Takashi Inaoka, Kozo Ochi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 4 Pages: 618-623

    • DOI

      10.1271/bbb.100854

    • NAID

      10028271088

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

    • Author(s)
      Kubo, Y., Inaoka, T., Hachiya, T., Miyake, M., Hase, S., Nakagawa, R., Hasegawa, H., Funane, K., Sakakibara, Y., and Kimura, K
    • Journal Title

      J.Biosci.Bioeng

      Volume: (In press)

    • NAID

      110009614950

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis2011

    • Author(s)
      Inaoka, T. and Ochi, K
    • Organizer
      International Union of Microbiological Society 2011 Congress
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis2011

    • Author(s)
      Takashi Inaoka, Kozo Ochi
    • Organizer
      International Union of Microbiological Society 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌の物質生産におけるスカンジウムの効果2011

    • Author(s)
      稲岡隆史、越智幸三
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      福山
    • Year and Date
      2011-08-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 枯草菌の物質生産におけるスカンジウムの効果2011

    • Author(s)
      稲岡隆史、越智幸三
    • Organizer
      平成23年度グラム陽性菌ゲノム機能会議
    • Place of Presentation
      福山大学社会連携研究推進センター(広島県)
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 枯草菌の二次代謝制御機構に関する研究2010

    • Author(s)
      稲岡隆史
    • Organizer
      日本農芸化学会関東支部例会
    • Place of Presentation
      筑波大学(茨城県)
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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