Moleclar genetic analysis of eukaryotic molybdate transport systems in a model yeast Hansenula polymorpha and a model animal cell
| Project/Area Number |
22780089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MAESHIMA Masayoshi 名古屋大学, 大学院・生命農学研究科, 教授 (80181577)
FUJIWARA Toru 東京大学, 農学生命科学研究科, 教授 (80242163)
NIIMMI Tomoaki 名古屋大学, 大学院・生命農学研究科, 助教 (30377791)
TOMIOKA Rie 名古屋大学, 大学院・生命農学研究科, 助教 (40456588)
NAKANISHI Hanayo 愛知医科大学, 学際的痛みセンター・研究員/名古屋大学, 理学研究科・研究員
MAEDA Michiko 名古屋大学, 大学院・生命農学研究科, 大学院生
KAWASHIMA Terumi 名古屋大学, 大学院・生命農学研究科, 大学院生
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | モリブデン / 微量元素 / 膜輸送 |
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| Research Abstract |
Molybdenum (Mo) is an essential trace element for almost all living organisms. Whilst living cells are known to import inorganic molybdate oxyanion from the surrounding environment, the in vivo dynamics of cytosolic molybdate and/or transport systems remain poorly understood. We investigated molybdate transport system of a model unicellular yeast Hansenula polymorpha in this study. Results of genetic analysis suggested the yeast has at least three defferent type of molybdate transport. Also, we obtained three candidate genes for the molybdate transport those exhibited molybdate accumulation in heterologous expression systems. Furthermore, we developed a genetically encoded Forester-resonance-energy-transfer (FRET)-based nanosensor for molybdate. By using the nanosensor we demonstrated in vivo dynamics of molybdate in a model animal cell HEK293T . Analyses of the dynamics suggest that novel oxalate-sensitive- and sulfate-resistant- transporter(s) uptake molybdate in thecell.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor2013
Author(s)
Nakanishi, Y., Iida, S., Ueoka-Nakanishi, H., Niimi, T., Tomioka, R., Maeshima, M
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Journal Title
PLoS One
Volume: 8
Issue: 3
Pages: e58175-e58175
DOI
Related Report
Peer Reviewed
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[Presentation] Imaging analysis of molybdate in vivo by a genetically encoded FRET nanosensor2013
Author(s)
Nakanishi, Y., Kawashima, T., Iida, S., Fujiwara, T., Maeshima, M.
Organizer
International Workshop on Plant Membrane Biology XVI
Place of Presentation
Kurashiki Geibunkan, Kurashiki
Year and Date
2013-03-28
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