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Analysis of the glycosidases on the metabolism ofβ-L-arabinooligosaccharides in Bifidobacteria.

Research Project

Project/Area Number 22780094
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionKagoshima University

Principal Investigator

FUJITA Kiyotaka  鹿児島大学, 農学部, 助教 (20381189)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords糖質分解酵素 / 糖鎖 / プレバイオティクス / β-アラビノオリゴ糖鎖 / ビフィズス菌 / 腸内細菌 / エクステンシン / 資化性 / β-アラビノオリゴ糖 / アラビノシダーゼ / ヒドロキシプロリン / 代謝機構
Research Abstract

β-L-Arabinooligosaccharides are found in the sugar chains of extensin observed in plant cell wall fractions. In this study, we cloned and characterizedα-L-arabinofuranosidase, novelβ-L-arabinobiosidase and novelβ-L-arabinofuranosidase from Bifidobacterium longum. Furthermore, we revealed a novel degradation pathway forβ-L-arabinooligosaccharides in B. longum.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (13 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Characterization of a novelβ-L-arabinofuranosidase in Bifidobacterium longum : Functional elucidation of a DUF1680 family member2011

    • Author(s)
      Fujita, K., Takashi, Y., Obuchi, E., Kitahara, K., and Suganuma, T.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 38079-38085

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析2011

    • Author(s)
      藤田清貴、坂元志帆、小野祐樹、若尾雅広、隅田泰生、北原兼文、菅沼俊彦
    • Journal Title

      応用糖質科学

      Volume: 1(2) Pages: 153-158

    • NAID

      130004626883

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of aβ-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family2011

    • Author(s)
      Fujita, K., Sakamoto, S., Ono, Y., Wakao, M., Suda, Y., Kitahara, K., and Suganuma, T.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 5143-5150

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a novel β-L-arabinofuranosidase in Bifidobacterium longum : Functional elucidation of a DUF1680 family member2011

    • Author(s)
      Fujita, K., Takashi, Y., Obuchi, E., Kitahara, K., Suganuma, T.
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 44 Pages: 38079-38085

    • DOI

      10.1074/jbc.m111.248690

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of a β-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family.2011

    • Author(s)
      Fujita, K., Sakamoto, S., Ono, Y., Wakao, M., Suda, Y., Kitahara, K., Suganuma, T.
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 5143-5150

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] β-アラビノオリゴ糖鎖のBifidobacterium longumに対する増殖促進効果2012

    • Author(s)
      藤田清貴、奥山千代美、北原兼文、菅沼俊彦
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ビフィズス菌が生産するβ-アラビノオリゴ糖鎖の分解酵素群の解析2012

    • Author(s)
      藤田清貴
    • Organizer
      日本農芸化学会西日本支部奨励賞講演会
    • Place of Presentation
      福岡
    • Year and Date
      2012-01-21
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ビフィズス菌によるβ-アラビノオリゴ糖鎖の資化性と関連酵素群の解析2011

    • Author(s)
      藤田清貴、大渕衣里子、五反田孝平、北原兼文、菅沼俊彦
    • Organizer
      日本応用糖質科学会平成23年度大会
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ビフィズス菌由来β-L-アラビノフラノシダーゼのGlu366残基の役割2011

    • Author(s)
      藤田清貴、五反田孝平、大渕衣里子、北原兼文、菅沼俊彦
    • Organizer
      平成23年度日本農芸化学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of novel glycosidases involved in the degradation of Hyp-linked oligoarabinosides2011

    • Author(s)
      Fujita, K., Sakamoto, S., Takashi, Y., Ono, Y., Obuchi, E., Kitahara, K., and Suganuma, T.
    • Organizer
      9th CarbohydrateBioengineering Meeting(CBM9)
    • Place of Presentation
      Lisboa, Portugal
    • Year and Date
      2011-05-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of novel glycosidases involved in the degradation of Hyp-linked oligoarabinosides2011

    • Author(s)
      Fujita, K., Sakamoto, S., Takashi, Y., Ono, Y., Obuchi, E., Kitahara, K., Suganuma, T.
    • Organizer
      9th Carbohydrate Bioengineering Meeting (CBM9)
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2011-05-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] ビフィズス菌によるβ-アラビノオリゴ糖鎖の分解と代謝2011

    • Author(s)
      藤田清貴
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] β-アラビノオリゴ糖鎖の分解に関わる新規糖質分解酵素群の機能解析2010

    • Author(s)
      藤田清貴、坂元志帆、高由香里、小野祐樹、大渕衣里子、北原兼文、菅沼俊彦
    • Organizer
      日本生物工学会2010年度大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-29
    • Related Report
      2011 Final Research Report
  • [Presentation] β-アラビノオリゴ糖鎖の分解に関わる新規糖質分解酵素群の機能解析2010

    • Author(s)
      藤田清貴、坂元志帆、高由香里、小野祐樹、大渕衣里子、北原兼文、菅沼俊彦
    • Organizer
      日本生物工学会2010年度大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析2010

    • Author(s)
      藤田清貴、坂元志帆、高由香里、小野祐樹、若尾雅広、隅田泰生、北原兼文、菅沼俊彦
    • Organizer
      第17回糖質関連酵素化学シンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析2010

    • Author(s)
      藤田清貴、坂元志帆、高由香里、小野祐樹、若尾雅広、隅田泰生、北原兼文、菅沼俊彦
    • Organizer
      第17回糖質関連酵素化学シンポジウム
    • Place of Presentation
      静岡市
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビフィズス菌由来新規β-L-アラビノフラノシダーゼのクローニングと機能解析2010

    • Author(s)
      藤田清貴、高由香里、大渕衣里子、北原兼文、菅沼俊彦
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      静岡
    • Year and Date
      2010-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] ビフィズス菌由来新規β-L-アラビノフラノシダーゼのクローニングと機能解析2010

    • Author(s)
      藤田清貴、高由香里、大渕衣里子、北原兼文、菅沼俊彦
    • Organizer
      日本応用糖質学会平成22年度大会
    • Place of Presentation
      静岡市
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Book] 酵素的切断法・(2)O-グリコシド結合の酵素分解、「糖質II糖タンパク質実験法」生物薬科学実験講座4卷(川嵜敏祐編)2011

    • Author(s)
      藤田清貴、山本憲二
    • Total Pages
      4
    • Publisher
      廣川書店
    • Related Report
      2011 Annual Research Report
  • [Book] 第7章エンドAの構造から糖タンパク質合成の最適条件を探る、「バイオ医薬品開発における糖鎖技術」早川堯夫・掛樋一晃・平林淳監修2011

    • Author(s)
      竹川薫、藤田清貴
    • Total Pages
      8
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://hypba2.jimdo.com/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hypba2.jimdo.com/

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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