Study of enzymes and factors provide direct solubilization of chitin

• Project/Area Number: 22780097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Ichinoseki National College of Technology
• Principal Investigator: NAKAGAWA Yuko 一関工業高等専門学校, 物質化学工学科, 助教 (70435577)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: キチン / キチン分解酵素 / 糖化促進 / キチン結合タンパク / N-アセチルグルコサミン / 組換え酵素 / 酵素分解促進 / バイオマス変換 / 糖質 / 基質の物性評価 / 国際情報交換 / ノルウェー / 環境負荷低減 / キチン質 / 相乗効果 / 直接分解 / Streptomyces

Research Abstract

We revealed three chitin binding proteins (CBPs) from Streptomyces griseus boosted enzymatic solubilization of - and -chitin. The amount of (GlcNAc)2 from the best combination with one of the CBP and a chitinase was about 30 times higher than that without the CBP after 24 h incubation.We proved crystallinity was affected to -chitin solubilization by using a commercially available enzyme from S. griceus or the chitinolytic system in Serratia marcescens.

Research Products

• [Journal Article] α-キチンの「生合成の謎」に迫る (2012)
  • Author(s): 中川裕子
  • Journal Title: 生物工学会誌 Volume: 5月号 Pages: 263-263
  • NAID: 110008662252
  • Peer Reviewed
• [Journal Article] High cell-density expression system : A novel method for extracellular production of "difficult-to-express" proteins (2012)
  • Author(s): Kimata K., Yamaguchi M., Saito Y., Hata H., Miyake K., Yamane T., Nakagawa Y., Yano A., Ito K., and Kawarasaki Y
  • Journal Title: J. Biosci. Bioeng Volume: 113 Issue: 2 Pages: 154-159
  • DOI: 10.1016/j.jbiosc.2011.10.007
  • NAID: 110009418661
  • Peer Reviewed
• [Journal Article] Development of innovative technologies to decrease the environmental burdens associated with using chitin as a biomass resource:mechanochemical grinding and enzymatic degradation. (2011)
  • Author(s): Yuko S. Nakagawa, Yasuhiro Oyama, Nobuko Kon, Mitsuru Nikaido, Koichi Tanno, Jun Kogawa, Shoji Inomata, Ayano Masui, Akihiro Yamamura, Mitsuaki Kawaguchi, Yoshiharu Matahira, Kazuhide Totani
  • Journal Title: Carbohydrate polymers Volume: 83 Pages: 1843-1849
  • Peer Reviewed
• [Journal Article] Development of innovative technologies to decrease the environmental burdens associated with using chitin as a biomass resource : mechanochemical grinding and enzymatic degradation (2011)
  • Author(s): Yuko S.Nakagawa, Yasuhiro Oyama, Nobuko Kon, Mitsuru Nikaido, Koichi Tanno, Jun Kogawa, Shoji Inomata, Ayano Masui, Akihiro Yamamura, Mitsuaki Kawaguchi, Yoshiharu Matahira, Kazuhide Totani
  • Journal Title: Carbohydrate polymers Volume: 83 Pages: 1843-1849
  • Peer Reviewed
• [Presentation] Streptomyces griseus 由来のCBM33 SgCBP IV のキチン酵素分解の促進 (2013)
  • Author(s): 工藤まどか,中川裕子,GustavVaaje-Kolstad, Vincent G. H. Eijsink,戸谷一英
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-26
• [Presentation] Streptomyces griseus 由来のCBM33,SgCBP Vの機能解析 (2013)
  • Author(s): 千葉大介,畠山彩乃,戸谷一英,中川裕子
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-26
• [Presentation] Streptomyces griseus由来のCBM33, SgCBP IVのキチン酵素分解の促進 (2013)
  • Author(s): 工藤まどか
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] Streptomyces griseus由来のCBM33, SgCBP Vの機能解析 (2013)
  • Author(s): 千葉大介
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] 地域糖質資源の活用に向けた酵素分解系の開発 (2012)
  • Author(s): 中川裕子
  • Organizer: 第37回糖質科学懇話会
  • Place of Presentation: 江ノ島
  • Year and Date: 2012-11-30
• [Presentation] Synergistic effect of CBM33s from Streptomyces griseus for enzymatic chitin degradation. (2012)
  • Author(s): Y. S. NAKAGAWA, M. KUDO, G.VAAJE-KOLSTAD, V. G. H. EIJSINK, and K.TOTANI
  • Organizer: 6th Iberoamerican Chitin Symposium & 12th International Conference on Chitin and Chitosan
  • Place of Presentation: Fortaleza, Brazil
  • Year and Date: 2012-09-25
• [Presentation] 地域糖質資源を活用した機能性素材の開発 (2012)
  • Author(s): 戸谷一英,古関健一,中川裕子,長田光正,二階堂満
  • Organizer: 応用糖質科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-09-21
• [Presentation] キチンの酵素分解におけるStreptomyces griseus 由来のCBM33の相乗効果 (2012)
  • Author(s): 工藤まどか,中川裕子,Gustav Vaaje-Kolstad,Vincent G. H. Eijsink,戸谷一英
  • Organizer: 第26回キチン・キトサンシンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2012-07-13
• [Presentation] Serratia marcescensのキチン分解系においてメカノケミカル粉砕処理が与える効果 (2012)
  • Author(s): 中川裕子,Gustav Vaaje-Kolstad, Vincent G. H.Eijsink,戸谷一英
  • Organizer: 第26回キチン・キトサンシンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2012-07-13
• [Presentation] Streptomyces griseus 由来推定キチン結合タンパクの異種発現と機能解析 (2012)
  • Author(s): 工藤まどか, 中川裕子,鈴木一史,渡邉剛志,戸谷一英
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] Streptomyces griseus 由来のCBM33の機能解析 (2012)
  • Author(s): 中川裕子,工藤まどか, Vaaje-Kolstad Gustav,Eijsink Vincent, 戸谷一英
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] Streptomyces griseus由来推定キチン結合タンパクの異種発現と機能解析 (2012)
  • Author(s): 工藤まどか, 中川裕子, 鈴木一史, 渡邉剛志, 戸谷一英
  • Organizer: 2012年日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Streptomyces griseus由来のCBM33の機能解析 (2012)
  • Author(s): 中川裕子, 工藤まどか, Vaaje-Kolstad Gustav, Eijsink Vincent, 戸谷一英
  • Organizer: 2012年日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] One of CBM33s from Streptomyces griseus accelerated enzymatic chitin degradation. (2012)
  • Author(s): Madoka KUDO, Yuko NAKAGAWA
  • Organizer: International Symposium on Thechnology for Sustainability
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Study of synergistic activity for enzymatic chitin degradation (2012)
  • Author(s): Yuko NAKAGAWA, Saeko TOCHIGI, Madoka KUDO and Kazuhide TOTANI
  • Organizer: Norwegian biochemical society 48th contact meeting
  • Place of Presentation: Storefjell, Norway
• [Presentation] Synergistic effect of CBM33s from Streptomyces griseus for enzymatic chitin degradation (2012)
  • Author(s): Yuko NAKAGAWA
  • Organizer: 6th Iberoamerican Chitin Symposium & 12th International Conference on Chitin and Chitosan
  • Place of Presentation: Fortaleza, Brazil
• [Presentation] One of CBM33s from Streptomyces griseus accelerated enzymatic chitin degradation (2012)
  • Author(s): Madoka KUDO
  • Organizer: International Symposium on Thechnology for Sustainability
  • Place of Presentation: Bangkok, Thailand
• [Presentation] キチンの酵素分解におけるStreptomyces griseus由来のCBM33の相乗効果 (2012)
  • Author(s): 工藤まどか
  • Organizer: 第26回 キチン・キトサンシンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] Serratia marcescensのキチン分解系においてメカノケミカル粉砕処理が与える効果 (2012)
  • Author(s): 中川裕子
  • Organizer: 第26回 キチン・キトサンシンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] Study of synergistic activity for enzymatic chitin degradation (2012)
  • Author(s): Yuko Nakagawa
  • Organizer: Norwegian biochemical society 48^<th> contact meeting
  • Place of Presentation: Storefjell, Norway
• [Presentation] キチン質の酵素糖化における相乗効果の検討日本農芸化学会年会 (2011)
  • Author(s): 中川裕子 、栃木佐枝子、工藤まどか、戸谷一英
  • Organizer: 日本農芸化学会2011年度大会講演要旨集
  • Year and Date: 2011-03-05
• [Presentation] キチン質の酵素糖化における相乗効果の検討 (2011)
  • Author(s): 中川裕子
  • Organizer: 日本農芸化学会年会
  • Place of Presentation: (日本農芸化学会2011年度大会講演要旨集)
  • Year and Date: 2011-03-05
• [Presentation] キチン質の酵素糖化に相乗効果をもたらす因子の解析 (2011)
  • Author(s): 工藤まどか,栃木佐枝子,戸谷一英,中川裕子
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良
• [Presentation] Study of synergistic activity for enzymatic chitin degradation (2011)
  • Author(s): Yuko Nakagawa, Saeko Tochigi, Madoka Kudo,and Kazuhide Totani
  • Organizer: 21st International symposium on glycoconjugates
  • Place of Presentation: Vienna, Austria
• [Presentation] Study of synergistic activity for enzymatic chitin degradation (2011)
  • Author(s): Yuko Nakagawa, Saeko Tochigi, Madoka Kudo, Kazuhide Totani
  • Organizer: 21^<st> International symposium on glycoconjugates
  • Place of Presentation: Vienna, Austria
• [Presentation] キチン質の酵素糖化に相乗効果をもたらす因子の解析 (2011)
  • Author(s): 工藤まどか, 栃木佐枝子, 戸谷一英, 中川裕子
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
• [Presentation] TOTANI Expression analysis of chitin inducible genes and study of synergistic activity on chitin degradation (2010)
  • Author(s): Y. NAKAGAWA, N. KON, N. YOSHIDA, M.NIKAIDO, A. MASUI, A. YAMAMURA, M.KAWAGUCHI, Y. MATAHIRA, and K.
  • Organizer: Mechanochemical grinding and enzymatic degradation. 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張
• [Presentation] Advanced utilization of chitin resources with technology for reducing environmental loads; (2010)
  • Author(s): Kazuhide TOTANI, Yuko NAKAGAWA,Nobuko KON, Yasuhiro OYAMA, Mitsuru NIKAIDO, Mitsumasa OSADA, Mikio KAIHARA, Koichi TANNO, Jun KOGAWA,Shoji INOMATA, Ayano MASUI, Akihiro YAMAMURA, Mitsuaki KAWAGUCHI and Yoshiharu MATAHIRA
  • Organizer: Mechanochemical treatment and mechanism of enzymatic degradation. 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張
• [Presentation] Pretreatments and enzymatic degradation of chitin. (2010)
  • Author(s): Kazuhide Totani, Yuko Nakagawa, Mitsumasa Osada, Mikio Kaihara, Mitsuru Nikaido, Jun Kogawa, Shoji Inomata, Mitsuaki Kawaguchi,Ayano Masui, Akihiro Yamamura, Yoshiharu Matahira
  • Organizer: Plant Polysaccharide and Applied Glycoscience
• [Presentation] キチン質の前処理と酵素分解 (2010)
  • Author(s): 中川裕子 、長田光正、貝原巳樹雄、二階堂満、粉川潤、猪俣尚治、増井彩乃、山村昭博、川口光朗、又平芳春、戸谷一英
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京
• [Presentation] キチン酵素糖化に関与するStreptomyces griseus由来の因子 (2010)
  • Author(s): 栃木佐枝子、中川裕子、二階堂満、増井彩乃、山村昭博、川口光朗、又平芳春、戸谷一英
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学
• [Presentation] キチン質の前処理と酵素分解 (2010)
  • Author(s): 中川裕子
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学(東京都)
• [Presentation] キチン酵素糖化に関与するStreptomyces griseus由来の因子 (2010)
  • Author(s): 栃木佐枝子
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学(東京都)
• [Presentation] Expression analysis of chitin inducible genes and study of synergistic activity on chitin degradation Mechanochemical grinding and enzymatic degradation. (2010)
  • Author(s): 中川裕子
  • Organizer: 25th International carbohydrate Symposium
  • Place of Presentation: 幕張メッセ(千葉県)
• [Remarks]
• [Remarks] ReaD & Researchmap
  • URL: http://researchmap.jp/yukonakagawa/?lang=japanese
• [Remarks] 一関工業高等専門学校
  • URL: http://www.ichinoseki.ac.jp/gyoseki/che/NakagawaYuko.html