Characterization and structural analysis of novel dye-linked L-proline dehydrogenase
| Project/Area Number |
22780098
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | University of Fukui (2011-2012)
Yonago National College of Technology (2010) |
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Principal Investigator |
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| Research Collaborator |
SAKURABA Haruhiko 香川大学, 農学部, 教授 (90205823)
OHSHIMA Toshihisa 九州大学, 農学研究科, 教授 (10093345)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 超好熱菌 / 色素依存性脱水素酵素 / L-プロリン脱水素酵素 / バイオセンサー / バイオ電池 |
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| Research Abstract |
Until now, two different types of Dye-ProDHs have been discovered in hyperthermophile. We have found the third novel type of Dye-ProDH whose molecular composition and properties were completelydifferent. Structure determination was performed. As the result, we succeeded the structural determination at high resolution. In addition, we succeeded the identification of the amino acid which is involved in substrate affinity and electron-transfer of the enzyme.
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Report
(4 results)
Research Products
(19 results)
