| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Artificial glycoclusters with various backbones(γ-polyglutamic acid, glycopolymer, and EGTA etc…) and multivalent sialyloligosaccharide units have been chemoenzymatically synthesized as potential polymeric inhibitors of infection by influenza virus. Furthermore, in order to synthesize an artificial sialoglycoclusters, we developed a large-scale production of ratα2, 3-orα2, 6-sialyltransferases(α2, 3-orα2, 6-SiaTs). Theα2, 3-orα2, 6-SiaTs were expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease-and chitinase-deficient Bombyx mori nucleopolyhedrovirus(Bm NPV-CP-Chi-) bacmid. The expressedα2, 3-SiaT andα2, 6-SiaT were used for sialylation of asialoglycoclusters.
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