Functional design of influenza virus blocker utilizing glycans

• Project/Area Number: 22780101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Shizuoka University
• Principal Investigator: OGATA Makoto 静岡大学, 創造科学技術大学院, 特任助教 (10532666)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子認識 / 糖鎖 / インフルエンザウイルス / ナノバイオ / 糖鎖クラスター効果 / 生体適合材料 / 感染症 / 酵素化学合成 / Glycotechnology / Influenza virus / Glycomimetics / Molecular design / Glycomaterial / Chemoenzymatic synthesis / Glycoconjugate / Multivalency effect

Research Abstract

Artificial glycoclusters with various backbones(γ-polyglutamic acid, glycopolymer, and EGTA etc…) and multivalent sialyloligosaccharide units have been chemoenzymatically synthesized as potential polymeric inhibitors of infection by influenza virus. Furthermore, in order to synthesize an artificial sialoglycoclusters, we developed a large-scale production of ratα2, 3-orα2, 6-sialyltransferases(α2, 3-orα2, 6-SiaTs). Theα2, 3-orα2, 6-SiaTs were expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease-and chitinase-deficient Bombyx mori nucleopolyhedrovirus(Bm NPV-CP-Chi-) bacmid. The expressedα2, 3-SiaT andα2, 6-SiaT were used for sialylation of asialoglycoclusters.

Research Products

• [Journal Article] Design and synthesis of high-avidity tetravalent glycoclusters as probes for Sambucus sieboldiana agglutinin and characterization of their binding properties. (2012)
  • Author(s): Ogata, M., Yano, M., Umemura, S., Murata, T., Park, E. Y., Kobayashi, Y., Asai, T., Oku, N., Nakamura, N., Matsuo, I., Usui, T.
  • Journal Title: Bioconjugate Chem. Volume: 23 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Park, E. Y. Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary Tran activator. (2012)
  • Author(s): Kato, T., Manohar, S. L., Kanamasa, S., Ogata, M.
  • Journal Title: J. Biosci. Bioeng. Volume: 113 Pages: 694-696
  • Peer Reviewed
• [Journal Article] Design and synthesis of high-avidity tetravalent glycoclusters as probes for Sambucus sieboldiana agglutinin and characterization of their binding properties (2012)
  • Author(s): Ogata, M., Yano, M., Umemura, S., Murata, T., Park, E.Y., Kobayashi, Y., Asai, T., Oku, N., Nakamura, N., Matsuo, I., Usui, T.
  • Journal Title: Bioconjugate Chem. Volume: 23 Issue: 1 Pages: 97-105
  • DOI: 10.1021/bc200440e
  • Peer Reviewed
• [Journal Article] Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and co-expression with the primary transactivator (2012)
  • Author(s): Tatsuya Kato, Suganthi Lavender Manohar, Shin Kanamasa, Makoto Ogata, and Enoch Y.Park
  • Journal Title: J. Biosci. Bioeng. Volume: 113巻 Issue: 6 Pages: 694-696
  • DOI: 10.1016/j.jbiosc.2012.01.010
  • Peer Reviewed
• [Journal Article] Label-free detection of oligosaccharide-lectin interaction using plasmonic optical device for glycomics application. (2011)
  • Author(s): Endo, T., Matsuda, S., Obara, T., Chuma, Y., Ogata, M., Yanagida, Y., Hatsuzawa, T., Usui, T.
  • Journal Title: Sensor. Mater. Volume: 23 Pages: 135-146
  • Peer Reviewed
• [Journal Article] Label-free detection of ollgosaccharide-lectin interaction using plasmonic optical device for glycomics application (2011)
  • Author(s): Endo, T., Matsuda, S., Obara, T, Chuma, Y., Ogata, M., Yanagida, Y., Hatsuzawa,T., Usui, T.
  • Journal Title: Sensor.Mater. Volume: 23 Pages: 135-146
  • Peer Reviewed
• [Journal Article] Lacto sylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I form Hypocrea jecorina and their properties. (2010)
  • Author(s): Ogata, M., Kameshima, Y., Hattori, T., Michishita, K., Suzuki, T., Kawagishi, H., Totani, K., Hiratake, J., Usui, T.
  • Journal Title: Carbohydr. Res. Volume: 345 Pages: 2623-2629
  • Peer Reviewed
• [Journal Article] Molecular design of fluorescent labeled glycosides as acceptor substrates for sialyl transferases. (2010)
  • Author(s): Ogata, M., Obara, T., Chuma, Y., Murata, T., Park, E. Y., Usui, T.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 74 Pages: 2287-2292
  • NAID: 10027561679
  • Peer Reviewed
• [Journal Article] Molecular design of N-linked tetravalent glycosides bearing N-acetylglucosamine, N, N'-diacetylchitobiose and N-acetyl lactosamine : Analysis of cross-linking activities with WGA and ECA lectins. (2010)
  • Author(s): Masaka, R., Ogata, M., Misawa, Y., Yano, M., Hashimoto, C., Murata, T., Kawagishi, H., Usui, T.
  • Journal Title: Bioorg. Med. Chem. Volume: 18 Pages: 621-629
  • NAID: 120001905750
  • Peer Reviewed
• [Journal Article] Lactosylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I form Hypocrea jecorina and their properties. (2010)
  • Author(s): Ogata, M., Kameshima, Y., Hattori, T., Michishita, K., Suzuki, T., Kawagishi, H., Totani, K., Hiratake, J., Usui, T.
  • Journal Title: Carbohydr.Res. Volume: 345 Pages: 2623-2629
  • Peer Reviewed
• [Journal Article] Molecular design of fluorescent labeled glycosides as acceptor substrates for sialyltransferases. (2010)
  • Author(s): Ogata, M., Obara, T., Chuma, Y., Murata, T., Park, E.Y., Usui, T.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 2287-2292
  • NAID: 10027561679
  • Peer Reviewed
• [Journal Article] Chemoenzymatic synthesis of glycan-arranged polymeric inhibitors against influenza virus infection. (2010)
  • Author(s): Ogata, M., Murata, T., Park, E.Y., Usui, T
  • Journal Title: J.Appl.Glycosci. Volume: 57 Pages: 137-144
  • NAID: 10027617072
  • Peer Reviewed
• [Journal Article] Molecular design of N-linked tetravalent glycosides bearing N-acetylglucosamine, N,N'-diacetylchitobiose and N-acetyllactosamine : Analysis of cross-linking activities with WGA and ECA lectins. (2010)
  • Author(s): Masaka, R., Ogata, M., Misawa, Y., Yano, M., Hashimoto, C., Murata, T., Kawagishi, H., Usui, T.
  • Journal Title: Bioorg.Med.Chem. Volume: 18 Pages: 621-629
  • NAID: 120001905750
  • Peer Reviewed
• [Presentation] フコシル三糖及び五糖含有新規4価配位体の合成 (2012)
  • Author(s): 森大地,尾形慎,朴龍洙,碓氷泰市
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] シアロ型多価糖鎖配位体とニホンニワトコレクチンとの凝集メカニズムの解析 (2012)
  • Author(s): 梅村征一郎,尾形慎,村田健臣,朴龍洙,中村直樹,松尾一郎,碓氷泰市
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] シアリルオリゴ糖鎖含有デキストリンナノ粒子の合成およびインフルエンザウイルスとの結合能評価 (2012)
  • Author(s): 杉山尚弘,梅村征一郎,尾形慎,渡邊浩史,柳瀬美千代,鷹羽武史,碓氷泰市
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] レクチン結合親和力に基づくアシアロ型多価配糖体の分子設計 (2012)
  • Author(s): 田見祐一,中馬康志,尾形慎,村田健臣,碓氷泰市
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] Bm NPVバクミドシステムを用いたカイコでの哺乳類由来糖転移酵素の発現 (2011)
  • Author(s): 森大地,尾形慎,碓氷泰市,朴龍洙
  • Organizer: 日本糖質学会
  • Place of Presentation: ハイブ長岡(新潟県)
  • Year and Date: 2011-07-13
• [Presentation] BmNPVバクミドシステムを用いたカイコでの哺乳類由来糖転移酵素の発現 (2011)
  • Author(s): 森大地、尾形慎、碓氷泰市、朴龍洙
  • Organizer: 日本糖質学会
  • Place of Presentation: ハイブ長岡(新潟県)
  • Year and Date: 2011-07-13
• [Presentation] 二分岐二本鎖配糖体の設計に基づくECAレクチンとの結合親和性評価 (2011)
  • Author(s): 田見祐一,杉山尚弘,中馬康志,尾形慎,碓氷泰市
  • Organizer: 日本糖質学会
  • Place of Presentation: ハイブ長岡(新潟県)
  • Year and Date: 2011-07-12
• [Presentation] レクチンとの架橋複合体形成能を有する四価シアロ型糖鎖配糖体の機能設計 (2011)
  • Author(s): 尾形慎,矢野恵美,村田健臣,朴龍洙,中村直樹,松尾一郎,碓氷泰市
  • Organizer: 日本糖質学会
  • Place of Presentation: ハイブ長岡(新潟県)
  • Year and Date: 2011-07-11
• [Presentation] Lactosy lamidine-based affinity purification for cellulolytic enzymes EG I and CBH I and analysis of their condensation and transglycosylation reactions (2010)
  • Author(s): Ogata, M., Kameshima, Y., Hattori, T., Totani, K., Nakamura, T., Koshino, H., Hiratake, J., Usui, T
  • Organizer: Plant polysaccharide and applied glyco science workshop(PPAGW)
  • Place of Presentation: The Sasakawa Hall(東京都)
• [Presentation] Lactosylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I and analysis of their condensation and transglycosylation reactions. (2010)
  • Author(s): Ogata, M., Kameshima, Y., Hattori, T., Totani, K., Nakamura, T., Koshino, H., Hiratake, J., Usui, T.
  • Organizer: Plant polysaccharide and applied glycoscience workshop (PPAGW)
  • Place of Presentation: The Sasakawa Hall(東京都)
• [Book] Biosensors-Emerging Materials and Applications, Molecular design of multivalent glycosides bearing GlcNAc,(GlcNAc)_2 and LacNAc : Analysis of cross-linkingactivities with WGA and ECA lectins (2011)
  • Author(s): Ogata, M., Misawa, Y., Usui, T
  • Publisher: InTech
• [Book] Molecular design of multivalent glycosides bearing GlcNAc, (GlcNAc)_2 and LacNAc : Analysis of cross-linking activities with WGA and ECA lectins (2011)
  • Author(s): Ogata, M., Misawa, Y., Usui, T.
  • Total Pages: 18
  • Publisher: INTEC open access publisher Biosensors-Emerging Materials and Application ISBN978-953-307-328-6
• [Book] Molecular design of multivalent glycosides bearing GlcNAc, (GlcNAc)_2 and LacNAc : Analysis of cross-linking activities with WGA and ECA lectins. (2011)
  • Author(s): Ogata, M., Misawa, Y., Usui, T.
  • Total Pages: 18
  • Publisher: INTEC open access publisher Biosensors for Health, Enviroment and Biosecurity/Book 1 ISBN 978-953-307-155-8
• [Book] 糖鎖を活用したインフルエンザウイルス感染阻害剤の酵素化学合成 (2010)
  • Author(s): 尾形慎
  • Publisher: バイオサイエンスとインダストリー
• [Book] 糖鎖を活用したインフルエンザウイルス感染阻害剤の機能設計 (2010)
  • Author(s): 尾形慎
  • Publisher: 化学工業
• [Book] Chemoenzymatic synthesis of glycan-arranged polymeric inhibitors against influenza virus infection (2010)
  • Author(s): Ogata, M., Murata, T., Park, E. Y. Usui, T
  • Publisher: J. Appl. Glycosci
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/c/biochem/index.html
• [Remarks]
  • URL: http://wvvw.agr.shizuoka.ac.jp/c/biochem/index.html
• [Patent(Industrial Property Rights)] ウイルス阻害剤 (2011)
  • Inventor(s): 碓氷泰市,尾形慎,朴龍洙
  • Industrial Property Rights Holder: 国立大学法人静岡大学
  • Filing Date: 2011-02-25