The electrochemical detection method of the carbohydrate-protein interaction for carbohydrate microarray
Project/Area Number |
22780107
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
KOMBA Shiro 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (20332273)
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Project Period (FY) |
2010-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
Keywords | 分子認識 / 糖鎖 / 糖鎖チップ / 電気科学計測 |
Research Abstract |
I have synthesized various sugar linkers and these were examined for recognition of lectins by the surface plasmon resonance method and an electrical signal. As a results of the various examinations, the sugar linkers that consist of 11-mercaptoundecanoic acid, 4,7,10-trioxa-1,13-tridecandiamine, and lactose or maltose for a terminal beta-galactose or alpha-glucose respectively were the best structure for electrical measurement (SWV measurement). I have established the technique that detect interaction between sugar and its recognizing protein (lectin etc.) by electrical measurement.
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-12012
Author(s)
Kumano-Kuramochi, M., Shimozu, Y., Wakita, C., Ohnishi-Kameyama, M., Shibata, T., Matsunaga, S., Takano-Ishikawa, Y., Watanabe, J., Goto, M., Xie, Q., Komba, S., Uchida, K., and Machida, S.
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Journal Title
Biochem. J
Volume: 442
Issue: 1
Pages: 171-180
DOI
Related Report
Peer Reviewed
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[Presentation] 糖鎖チップの新時代2011
Author(s)
今場司朗
Organizer
日本応用糖質科学会平成23年度大会
Place of Presentation
北海道
Year and Date
2011-09-29
Related Report
Invited
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[Presentation] 糖鎖チップの新時代2011
Author(s)
今場司朗
Organizer
日本応用糖質科学会平成23年度大会
Place of Presentation
北海道大学(北海道)(招待講演)
Year and Date
2011-09-29
Related Report
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[Presentation] 糖脂質の新規精製法2010
Author(s)
今場司朗
Organizer
日本応用糖質科学会平成22年度大会
Place of Presentation
グランシップ(静岡県)
Year and Date
2010-09-16
Related Report
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