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Dual role of the main auxin biosynthesis pathway in plants

Research Project

Project/Area Number 22780108
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MASHIGUCHI Kiyoshi  独立行政法人理化学研究所, 生長制御研究グループ, 研究員 (00569725)

Research Collaborator KASAHARA Hiroyuki  独立行政法人理化学研究所, 生長制御研究グループ, 上級研究員
TANAKA Keita  東京農工大学, 大学院, 大学院生/理化学研究所研修生
YUNDE Zhao  University of California, San Diego, Professor
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords植物ホルモン / オーキシン / インドール-3-酢酸 / フェニル酢酸 / YUCCA / LC-ESI-MS/MS / シロイヌナズナ / フラビン含有モノオキシゲナーゼ / フラビンモノオキシゲナーゼ / インドール酢酸
Research Abstract

Auxins are a group of phytohormones that regulate many aspects of plant growth and development. Phenylacetic acid (PAA) has been known as a naturally-occurring auxin as well as indole-3-acetic acid (IAA), but its physiological function is still unknown. Here we elucidate that PAA is produced from phenylpyruvate by YUCCA flavin-containing monooxygenases, which catalyze a rate-limiting step in IAA biosynthesis. We further show that PAA is recognized by IAA receptors TIR1/AFBs, whereas not by IAA polar transporters, suggesting that IAA and PAA play different physiological roles in plants.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (38 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (27 results) Book (1 results) Remarks (4 results)

  • [Journal Article] The biochemical mechanism of auxin biosynthesis by an arabidopsis YUCCA flavin-containing monooxygenase.2013

    • Author(s)
      Dai X, Mashiguchi K, Chen Q, Kasahara H, Kamiya Y, Ojha S, DuBois J, Ballou D, Zhao Y
    • Journal Title

      J Biol Chem.

      Volume: 288(3) Pages: 1448-1457

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Biochemical Mechanism of Auxin Biosynthesis by an Arabidopsis YUCCA Flavin-containing Monooxygenase2013

    • Author(s)
      Dai X*, Mashiguchi K*, Chen Q, Kasahara H, Kamiya Y, Ojha S, DuBois J, Ballou D, Zhao Y.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288 Issue: 3 Pages: 1448-1457

    • DOI

      10.1074/jbc.m112.424077

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conversion of tryptophan to indole-3-acetic acid by TRYPTOPHAN AMINOTRANSFERASES OF ARABIDOPSIS and YUCCAs in Arabidopsis.2011

    • Author(s)
      Won C, Shen X, Mashiguchi K, Zheng Z, Dai X, Cheng Y, Kasahara H, Kamiya Y, Chory J, Zhao Y
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 108(45) Pages: 18518-18523

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The main auxin biosynthesis pathway in Arabidopsis.2011

    • Author(s)
      Mashiguchi K, Tanaka K, Sakai T, Sugawara S, Kawaide H, Natsume M, Hanada A, Yaeno T, Shirasu K, Yao H, McSteen P, Zhao Y, Hayashi K-i, Kamiya Y, Kasahara H
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 108(45) Pages: 18512-18517

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The main auxin biosynthesis pathway in Arabidopsis2011

    • Author(s)
      Mashiguchi, K., Tanaka, K., Sakai, T., Sugawara, S., Kawaide, H., Natsume, M., Hanada, H., Yaeno, T., Shirasu, K., Yao, H., Mc Steen, P., Zhao, Y., Hayashi, K., Kamiya, Y., Kasahara, H.
    • Journal Title

      Proc.Natl.Acad.Sci.USA.

      Volume: 108 Issue: 45 Pages: 18512-18517

    • DOI

      10.1073/pnas.1108434108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conversion of tryptophan to indole-3-acetic acid by TRYPTOPHAN AMINOTRANSFERASES OF ARABIDOPSIS and YUCCAs in Arabidopsis2011

    • Author(s)
      Christina Won, Xiangling Shen, Kiyoshi Mashiguchi, Zuyu Zheng, Xinhua Dai, Youfa Cheng, Hiroyuki Kasahara, Yuji Kamiya, Joanne Chory, Yunde Zhao
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 108(45) Issue: 45 Pages: 18518-18523

    • DOI

      10.1073/pnas.1108436108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] オーキシン生合成酵素YUCCA の生化学的解析2013

    • Author(s)
      増口潔, Yunde Zhao, 神谷勇治, 笠原博幸
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] オーキシン生合成酵素YUCCAの生化学的解析2013

    • Author(s)
      増口潔, Zhao Yunde, 神谷勇治, 笠原博幸
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学、岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] TAAとYUCによるインドール-3-酢酸の生合成2012

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子, 夏目雅裕, 川出洋, 花田篤志, 八丈野孝, 白須賢, Hong Yao, Paula Mcsteen, Yunde Zhao, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biochemical characterization of YUCCA flavin monooxygenases in auxin biosynthesis in Arabidopsis.2012

    • Author(s)
      Mashiguchi K, Zhao Y, Kamiya Y, Kasahara H
    • Organizer
      Auxin 2012
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Phenylacetic acid is a novel non-polar transport-type auxin in plants.2012

    • Author(s)
      Tanaka K, Mashiguchi K, Sugawara S, Hishiyama S, Sakai T, Hanada K, Kinoshita-Tsujimura K, Kakimoto T, Dai X, Kawaide H, Natsume M, Estelle M, Zhao Y, Hayashi K-i, Kamiya Y, Kasahara, H
    • Organizer
      Auxin 2012
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] TAA とYUC によるインドール-3-酢酸生合成経路の生化学的な解析2012

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子,夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第30回日本植物細胞分子生物学会
    • Place of Presentation
      奈良
    • Related Report
      2012 Final Research Report
  • [Presentation] Auxin biosynthesis by TAA and YUCCA families in plants.2012

    • Author(s)
      Mashiguchi K, Tanaka K, Tatsuya S, Sugawara S, Kawaide H, Natsume M, Hanada A, Yaeno T, Shirasu K, Yao H, McSteen P, Zhao Y, Hayashi K-i, Kamiya Y, Kasahara H
    • Organizer
      The 23rd International Conference on Arabidopsis Research (ICAR)
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Final Research Report
  • [Presentation] TAAとYUCによるインドール-3-酢酸の生合成2012

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子,夏目雅裕, 川出洋, 花田篤志, 八丈野孝, 白須賢, Hong Yao, Paula Mcsteen, Yunde Zhao, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Phenylacetic acid is a novel non-polar transport-type auxin in plants2012

    • Author(s)
      Tanaka K et al.
    • Organizer
      Auxin 2012
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Biochemical characterization of YUCCA flavin monooxygenases in auxin biosynthesis in Arabidopsis2012

    • Author(s)
      Mashiguchi K et al.
    • Organizer
      Auxin 2012
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Auxin biosynthesis by TAA and YUCCA families in plants2012

    • Author(s)
      Mashiguchi K et al.
    • Organizer
      The 23rd International Conference on Arabidopsis Research (ICAR)
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] Auxin biosynthesis by TAA and YUCCA families in plants2012

    • Author(s)
      Mashiguchi K et al.
    • Organizer
      Plant Vascular Development 2012
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Annual Research Report
  • [Presentation] YUCによるインドール-3-酢酸生合成経路の生化学的な解析2012

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子, 夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第30回日本植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学、奈良
    • Related Report
      2012 Annual Research Report
  • [Presentation] インドール-3-ピルビン酸(IPA)を介した植物のオーキシン生合成経路の解析2011

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子, 夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物におけるフェニル酢酸の生合成および代謝経路の解析2011

    • Author(s)
      菅原聡子, 増口潔, 田中慧太, 菱山正二郎, 花田篤志, 川出洋, 夏目雅裕, 酒井達也, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] フェニル酢酸のオーキシンシグナル伝達および輸送機構の解析2011

    • Author(s)
      田中慧太, 増口潔, 酒井達也, 夏目雅裕, 川出洋, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナのインドール-3-酢酸生合成経路の解明2011

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子,夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮
    • Related Report
      2012 Final Research Report
  • [Presentation] インドール-3-ピルビン酸(IPA) を介した植物のオーキシン生合成経路の解析2011

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子,夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物におけるフェニル酢酸の生合成および代謝経路の解析2011

    • Author(s)
      菅原聡子, 増口潔, 田中慧太, 菱山正二郎,花田篤志, 川出洋, 夏目雅裕, 酒井達也, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] フェニル酢酸のオーキシンシグナル伝達および輸送機構の解析2011

    • Author(s)
      田中慧太, 増口潔, 酒井達也, 夏目雅裕,川出洋, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物におけるフェニル酢酸生合成経路の解析2011

    • Author(s)
      増口潔, 菅原聡子, 田中慧太, 軸丸裕介,花田篤志, 夏目雅裕, 川出洋, 酒井達也, 神谷勇治, 林謙一郎, 笠原博幸
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物におけるフェニル酢酸の濃度調節機構の解析2011

    • Author(s)
      田中慧太, 増口潔, 菅原聡子, 軸丸裕介,夏目雅裕, 川出洋, 酒井達也, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナのインドール-3-酢酸生合成経路の解明2011

    • Author(s)
      増口潔, 田中慧太, 酒井達也, 菅原聡子, 夏目雅裕, 川出洋, 花田篤志, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      植物化学調節学会第46回大会
    • Place of Presentation
      宇都宮大学(宇都宮)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物におけるフェニル酢酸生合成経路の解析2011

    • Author(s)
      増口潔, 菅原聡子, 田中慧太, 軸丸裕介, 花田篤志, 夏目雅裕, 川出洋, 酒井達也, 神谷勇治, 林謙一郎, 笠原博幸
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      東北大学、仙台市
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物におけるフェニル酢酸の濃度調節機構の解析2011

    • Author(s)
      田中慧太, 増口潔, 菅原聡子, 軸丸裕介, 夏目雅裕, 川出洋, 酒井達也, 林謙一郎, 神谷勇治, 笠原博幸
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      東北大学、仙台市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dissection of auxin biosynthesis via indole-3-acetaldehyde in plants.2010

    • Author(s)
      Mashiguchi K, Hayashi K, Tanaka K, Sugawara S, Jikumaru Y, Hanada A, Natsume M, Kawaide H, Yunde Z, Kamiya Y, Kasahara H
    • Organizer
      20th International Conference on Plant Growth Substances (IPGSA)
    • Place of Presentation
      Tarragona, Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] Dissection of Auxin Biosynthesis via Indole-3-acetaldehyde in Plants2010

    • Author(s)
      Mashiguchi Kiyoshi, Hayashi Ken-ichiro, Tanaka Keita, Sugawara Satoko, Jikumaru Yusuke, Hanada Atsushi, Natsume Masahiro, Kawaide Hiroshi, Yunde Zhao, Kamiya Yuji, Kasahara Hiroyuki
    • Organizer
      20th International Conference on Plant Growth Substances (IPGSA 2010)
    • Place of Presentation
      Tarragona, Spain
    • Related Report
      2010 Annual Research Report
  • [Book] 植物のオーキシン生合成主経路の解明 : TAA およびYUCCA ファミリーによるインドール-3-酢酸の生合成. 化学と生物2012

    • Author(s)
      笠原博幸, 増口潔, 神谷勇治
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/grrt/

    • Related Report
      2012 Final Research Report
  • [Remarks] 理研PSC 生長制御研究グループ

    • URL

      http://labs.psc.riken.jp/grrt/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/grrt/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/grrt/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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