Analysis of carbohydrate recognition by Pradimicins, natural small molecules with lectin-like properties
Project/Area Number |
22780109
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
NAKAGAWA Yu 独立行政法人理化学研究所, 伊藤細胞制御化学研究室, 専任研究員 (90452284)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | Antibiotic / Carbohydrate recognition / Lectin / Natural product / Pradimicin / Solid-state NMR / Pradimicin A / 抗生物質 / 放線菌 / 糖受容体 / 天然物 / 固体NMR / 凝集 / 固相相互作用解析 |
Research Abstract |
Pradimicins(PRMs) are natural antibiotics that recognize D-mannose(Man) in the presence of Ca^<2+> ion. In this study, a new strategy for analyzing molecular interaction of PRMs with Man in the solid state has been developed. The solid-state NMR analyses of the complexes of PRMs with Man revealed that PRMs bind Man in a Ca^<2+>-mediated manner, and successfully identified the Man binding site of PRMs.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Solid-state NMR analysis of calcium and D-mannose binding of BMY-28864, a water-soluble analogue of pradimicin A2012
Author(s)
Nakagawa, Y., Doi, T., Takegoshi, K., Igarashi, Y., and Ito, Y.
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Journal Title
Bioorg. Med. Chem. Lett.
Volume: 22
Pages: 1038-1041
Related Report
Peer Reviewed
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[Journal Article] Mapping of the Primary Mannose-Binding Site of Pradimicin A2011
Author(s)
Nakagawa, Y., Doi, T., Masuda, Y., Takegoshi, K., Igarashi, Y., and Ito, Y.
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Journal Title
J. Am. Chem. Soc.
Volume: 133
Pages: 17485-17493
Related Report
Peer Reviewed
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[Journal Article] Solid-state NMR Spectroscopic Analysis of the Ca^<2+>-dependent Mannose Binding of Pradimicin A2011
Author(s)
Nakagawa, Y., Masuda, Y., Yamada, K., Doi, T., Takegoshi, K., Igarashi, Y., and Ito, Y.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 50
Pages: 6084-6088
Related Report
Peer Reviewed
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[Journal Article] Solid-state NMR spectroscopic analysis of the Ca^<2+>-dependent mannose binding of pradimicin A2011
Author(s)
Nakagawa, Y., Masuda, Y., Yamada, K., Doi, T., Takegoshi, K., Igarashi, Y., Ito, Y.
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Journal Title
Angewandte Chemie International Edition
Volume: 50
Issue: 27
Pages: 6084-6088
DOI
Related Report
Peer Reviewed
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[Journal Article] Mapping of the primary mannose binding site of pradimicin A2011
Author(s)
Nakagawa, Y., Doi, T., Masuda, Y., Takegoshi, K., Igarashi, Y., Ito, Y.
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Journal Title
Journal of the American Chemical Society
Volume: 133
Issue: 43
Pages: 17485-17493
DOI
Related Report
Peer Reviewed
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