Analysis of interaction between flavonoids and proteins for elucidating their physiological expression mechanisms.
Project/Area Number |
22780122
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Food science
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Research Institution | University of Shizuoka |
Principal Investigator |
ISHII Takeshi 静岡県立大学, 食品栄養科学部, 助教 (50448700)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | フラボノイド類 / カテキン類 / 標的蛋白質 / 相互作用 / 結合親和性 / フラボノイド / テアフラビン類 / 蛋白質 / 標的分子 / 共有結合 / 親和性 / 血清アルブミン / プロテオミクス |
Research Abstract |
Previous research has indicated that several flavonoids form complexes with target molecule such as protein in blood and organs, and differences in binding affinity toward the target molecule are believed to determine their biological activity. In this study, the interaction of catechins and their analogs to proteins were evaluated and the relationship between the chemical structure of each catechin and its binding affinity were investigated. The study indicates the galloyl group, the number of hydroxyl groups on the B-ring, and the configuration at C-2 of each catechin influence binding affinity for the serum proteins and the several cellular proteins.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] Human Serum Albumin as an Antioxidant in the Oxidation of (-)-Epigallocatechin Gallate: Participation of Reversible Covalent Binding for Interaction and Stabilization2011
Author(s)
Takeshi Ishii, Tatsuya Ichikawa, Kanako Minoda, Koji Kusaka, Sohei Ito, Yukiko Suzuki, Mitsugu Akagawa, Kazuki Mochizuki, Toshinao Goda, and Tsutomu Nakayama
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 1
Pages: 100-106
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Electrophilic reactivity of tea catechins toward protein sulfhydryls2011
Author(s)
Mori, T., Ishii, T., Katsumata, T., Akagawa, M., Nakamura, Y., and Nakayama, T
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Journal Title
Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science
Related Report
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[Journal Article] (-)-Epigallocatechin-3-gallate inhibits EGF signaling via covalent binding to EGF receptor2011
Author(s)
Maeda, T., Kubo, Y., Kimura, K., Tanaka, T., Ishii, T., and Akagawa, M
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Journal Title
Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science
Related Report
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[Journal Article] Influence of the galloyl moiety in tea catechins on binding affinity for human serum albumin2010
Author(s)
Minoda, K., Ichikawa, T., Katsumata, T., Onobori, K., Mori, T., Suzuki, Y., Ishii, T., and Nakayama, T
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Journal Title
J. Nutr. Sci. Vitaminol
Volume: 56
Pages: 331-334
NAID
URL
Related Report
Peer Reviewed
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[Journal Article] Structural characteristics of green tea catechins for formation of protein carbonyl in human serum albumin2010
Author(s)
Takeshi Ishii, Taiki Mori, Tatsuya Ichikawa, Maiko Kaku, Koji Kusaka, Yoshinori Uekusa, Mitsugu Akagawa, Yoshiyuki Aihara, Takumi Furuta, Toshiyuki Wakimoto, Toshiyuki Kan, and Tsutomu Nakayama
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Journal Title
Bioorg. Med. Chem
Volume: 18
Issue: 14
Pages: 4892-4896
DOI
Related Report
Peer Reviewed
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[Journal Article] Binding affinity of tea catechins for HSA : characterization by high-performance affinity chromatography with immobilized albumin column2010
Author(s)
Ishii, T., Minoda, K., Bae, M.-J., Mori, T., Uekusa, Y., Ichikawa, T., Aihara, Y., Furuta, T., Wakimoto, T., Kan, T., and Nakayama, T
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Journal Title
Mol. Nutr. Food Res
Volume: 54
Issue: 6
Pages: 816-822
DOI
Related Report
Peer Reviewed
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[Presentation] (-)-Epigallocatechin-3-gallate suppresses growth of AZ521 human gastric cancer cells by targeting the DEAD box RNA helicase p682011
Author(s)
Akagawa, M., Tanaka, T., Mizuno, D., Mori, T., Yamaji, R., Nakamura, Y., Kumazawa, S., Nakayama, T., and Ishii, T
Organizer
2011 International Conference of Food Factors
Place of Presentation
Taipei, Taiwan
Year and Date
2011-11-21
Related Report
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