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Analysis of interaction between flavonoids and proteins for elucidating their physiological expression mechanisms.

Research Project

Project/Area Number 22780122
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionUniversity of Shizuoka

Principal Investigator

ISHII Takeshi  静岡県立大学, 食品栄養科学部, 助教 (50448700)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsフラボノイド類 / カテキン類 / 標的蛋白質 / 相互作用 / 結合親和性 / フラボノイド / テアフラビン類 / 蛋白質 / 標的分子 / 共有結合 / 親和性 / 血清アルブミン / プロテオミクス
Research Abstract

Previous research has indicated that several flavonoids form complexes with target molecule such as protein in blood and organs, and differences in binding affinity toward the target molecule are believed to determine their biological activity. In this study, the interaction of catechins and their analogs to proteins were evaluated and the relationship between the chemical structure of each catechin and its binding affinity were investigated. The study indicates the galloyl group, the number of hydroxyl groups on the B-ring, and the configuration at C-2 of each catechin influence binding affinity for the serum proteins and the several cellular proteins.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (35 results)

All 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 11 results) Presentation (14 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Epigallocatechin-3-gallate induces growth inhibition of human gastric cancer cell line AZ521 cells by targeting the DEAD box RNA helicase p682011

    • Author(s)
      Tomoko Tanaka, Takeshi Ishii, Daisuke Mizuno, Taiki Mori, Ryoichi Yamaji, Yoshimasa Nakamura, Shigenori Kumazawa, Tsutomu Nakayama, and Mitsugu Akagawa
    • Journal Title

      Free Radic. Biol. Med

      Volume: 50 Issue: 10 Pages: 1324-1335

    • DOI

      10.1016/j.freeradbiomed.2011.01.024

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Serum Albumin as an Antioxidant in the Oxidation of (-)-Epigallocatechin Gallate: Participation of Reversible Covalent Binding for Interaction and Stabilization2011

    • Author(s)
      Takeshi Ishii, Tatsuya Ichikawa, Kanako Minoda, Koji Kusaka, Sohei Ito, Yukiko Suzuki, Mitsugu Akagawa, Kazuki Mochizuki, Toshinao Goda, and Tsutomu Nakayama
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 1 Pages: 100-106

    • DOI

      10.1271/bbb.100600

    • NAID

      10027896843

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of tea catechins with serum proteins : Development and application of a new probe for understanding the action of catechins2011

    • Author(s)
      Katsumata, T., Ishii, T., and Nakayama, T
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] Structural characteristics of tea catechins that affect their binding affinity with cyclodextrins2011

    • Author(s)
      Kusaka, K., Ishii, T., and Nakayama, T
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] Stability of(-)-epigallocatechin gallate in aqueous solutions containing food constituents : Effect of synergy between antioxidant property and binding capacity2011

    • Author(s)
      Kusaka, K., Ishii, T., Hiramatsu, M., and Nakayama, T
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] Electrophilic reactivity of tea catechins toward protein sulfhydryls2011

    • Author(s)
      Mori, T., Ishii, T., Katsumata, T., Akagawa, M., Nakamura, Y., and Nakayama, T
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] A search for the high-affinity proteins of tea catechins in human cell lysates2011

    • Author(s)
      Katsumata, T., Ishii, T., and Nakayama, T
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] (-)-Epigallocatechin-3-gallate inhibits EGF signaling via covalent binding to EGF receptor2011

    • Author(s)
      Maeda, T., Kubo, Y., Kimura, K., Tanaka, T., Ishii, T., and Akagawa, M
    • Journal Title

      Proceedings of the 4th International Conference on O-CHA(Tea) Culture and Science

    • Related Report
      2011 Final Research Report
  • [Journal Article] Human serum albumin as an antioxidant in the oxidation of (-)-epigallocatechin gallate : participation of reversible covalent binding for interaction and stabilization.2011

    • Author(s)
      Ishii, T., et al.
    • Journal Title

      Bioscience, Biotechnology,and Biochemistry

      Volume: 75 Pages: 100-106

    • NAID

      10027896843

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Binding of Tea Catechins to Protein Thiols: The Relationship between Stability and Electrophilic Reactivity2010

    • Author(s)
      Taiki Mori, Takeshi Ishii, Mitsugu Akagawa, Yoshimasa Nakamura, and Tsutomu Nakayama
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Issue: 12 Pages: 2451-2456

    • DOI

      10.1271/bbb.100509

    • NAID

      10027728118

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the galloyl moiety in tea catechins on binding affinity for human serum albumin2010

    • Author(s)
      Minoda, K., Ichikawa, T., Katsumata, T., Onobori, K., Mori, T., Suzuki, Y., Ishii, T., and Nakayama, T
    • Journal Title

      J. Nutr. Sci. Vitaminol

      Volume: 56 Pages: 331-334

    • NAID

      10026966230

    • URL

      https://www.jstage.jst.go.jp/article/jnsv/56/5/56_5_331/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural characteristics of green tea catechins for formation of protein carbonyl in human serum albumin2010

    • Author(s)
      Takeshi Ishii, Taiki Mori, Tatsuya Ichikawa, Maiko Kaku, Koji Kusaka, Yoshinori Uekusa, Mitsugu Akagawa, Yoshiyuki Aihara, Takumi Furuta, Toshiyuki Wakimoto, Toshiyuki Kan, and Tsutomu Nakayama
    • Journal Title

      Bioorg. Med. Chem

      Volume: 18 Issue: 14 Pages: 4892-4896

    • DOI

      10.1016/j.bmc.2010.06.021

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding affinity of tea catechins for HSA : characterization by high-performance affinity chromatography with immobilized albumin column2010

    • Author(s)
      Ishii, T., Minoda, K., Bae, M.-J., Mori, T., Uekusa, Y., Ichikawa, T., Aihara, Y., Furuta, T., Wakimoto, T., Kan, T., and Nakayama, T
    • Journal Title

      Mol. Nutr. Food Res

      Volume: 54 Issue: 6 Pages: 816-822

    • DOI

      10.1002/mnfr.200900071

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] お茶の効能を分子レベルで解明する2010

    • Author(s)
      石井剛志、菅敏幸
    • Journal Title

      ファルマシア

      Volume: 46 Pages: 1143-1148

    • NAID

      110009867673

    • Related Report
      2011 Final Research Report
  • [Journal Article] Binding affinity of tea catechins for HSA : characterization by high-performance affinity chromatography with immobilized albumin column.2010

    • Author(s)
      Ishii, T., et al.
    • Journal Title

      Molecular Nutrition and Food Research

      Volume: 54 Pages: 816-822

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural characteristics of green tea catechins for formation of protein carbonyl in human serum albumin.2010

    • Author(s)
      Ishii, T., et al.
    • Journal Title

      Bioorganic and Medicinal Chemistry

      Volume: 18 Pages: 4892-4896

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the galloyl moiety in tea catechins on binding affinity for human serum albumin.2010

    • Author(s)
      Minoda, K., et al.
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 56 Pages: 331-334

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent binding of tea catechins to protein thiols : Relationship between stability and electrophilic reactivity.2010

    • Author(s)
      Mori, T., et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Pages: 2451-2456

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 紅茶テアフラビン類の蛋白質に対する反応性2012

    • Author(s)
      石井剛志, ほか
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] (-)-Epigallocatechin-3-gallate suppresses growth of AZ521 human gastric cancer cells by targeting the DEAD box RNA helicase p682011

    • Author(s)
      Akagawa, M., Tanaka, T., Mizuno, D., Mori, T., Yamaji, R., Nakamura, Y., Kumazawa, S., Nakayama, T., and Ishii, T
    • Organizer
      2011 International Conference of Food Factors
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] エピガロカテキンガレートと細胞膜との相互作用に血清蛋白質が及ぼす影響2011

    • Author(s)
      山本祥平、石井剛志、勝間田知治、中山勉
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] エピガロカテキンガレートと相互作用する血清蛋白質:アルブミンとトランスサイレチンの関係2011

    • Author(s)
      山本祥平、勝間田知治、石井剛志、中山勉
    • Organizer
      第8回日本カテキン学会総会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] カテキン類とカフェインの生体成分に対する結合親和性評価2011

    • Author(s)
      北村実穂、石井剛志、中山勉
    • Organizer
      第8回日本カテキン学会総会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] テアフラビン類と生体成分との相互作用解析2011

    • Author(s)
      上園博美、石井剛志、中山勉
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] カテキン類結合セファロースを用いた標的蛋白質の探索2011

    • Author(s)
      勝間田知治、石井剛志、中山勉
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 紅茶テアフラビン類の蛋白質に対する反応性2011

    • Author(s)
      林美香、石井剛志、平松未宇、中山勉
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Interaction of tea catechins with serum and cell proteins2010

    • Author(s)
      Katsumata, T., Ishii, T., and Nakayama, T
    • Organizer
      Biochemistry and Molecular Biology 2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural characteristics of tea catechins for formation of protein carbonyl in human serum albumin2010

    • Author(s)
      Mori, T., Ishii, T., and Nakayama, T
    • Organizer
      Biochemistry and Molecular Biology 2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] カテキン類とヒト血清アルブミンとの結合特性2010

    • Author(s)
      勝間田知治、石井剛志、蓑田香奈子、森大気、伊藤創平、中山勉
    • Organizer
      第15回日本フードファクター学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-10-04
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] カテキン類と親和性の高い細胞タンパク質の探索2010

    • Author(s)
      勝間田知治、石井剛志、森大気、中山勉
    • Organizer
      第57回日本食品科学工学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-03
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] カテキン類と血清タンパク質との相互作用2010

    • Author(s)
      石井剛志、勝間田知治、日下耕二、市川達也、尾登賢一、蓑田香奈子、加来麻衣子、森大気、伊藤創平、中山勉
    • Organizer
      第64回日本栄養・食糧学会大会
    • Place of Presentation
      徳島
    • Year and Date
      2010-05-23
    • Related Report
      2011 Final Research Report
  • [Presentation] カテキン類と血清タンパク質との相互作用2010

    • Author(s)
      石井剛志, ほか
    • Organizer
      第64回日本・栄養食糧学会大会
    • Place of Presentation
      徳島
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://sfns.u-shizuoka-ken.ac.jp/foodbioc/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://sfns.u-shizuoka-ken.ac.jp/foodbioc/index.html

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] ポリフェノール類化合物の苦渋味の評価方法

    • Inventor(s)
      中山勉、石井剛志、山田智
    • Industrial Property Rights Holder
      中山勉、石井剛志、山田智
    • Industrial Property Number
      2011-253607
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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