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Identification and assessment of probiotic factor(s) via multiple omics approach.

Research Project

Project/Area Number 22780132
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

FUKUDA Shinji  独立行政法人理化学研究所, 免疫系構築研究チーム, 研究員 (80435677)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsビフィズス菌 / 酢酸 / O157 / マルチオーミクス / プロバイオティクス / 腸内細菌 / 相互作用 / 病原性大腸菌 / マルチオミクス
Research Abstract

In order to demonstrate the effect of the bifidobacterial genes encoding 'probiotic transpoter', we generated the genes deficient or overexpressing bifidobacterial strains and assessed them using Escherichia coli O157 : H7 lethal infection murine model. Our experiments clearly showed that the 'probiotic transpoter' largely contributes to the production of acetate by colonized bifidobacteria in the murine colon, and it could prevent the inflammation provoked by Escherichia coli O157 : H7 infection.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (47 results)

All 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 10 results) Presentation (25 results) Book (7 results) Remarks (1 results)

  • [Journal Article] Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters2012

    • Author(s)
      Fukuda, S., Toh, H., Taylor, T. D., Ohno, H., and Hattori, M
    • Journal Title

      Gut Microbes

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters2012

    • Author(s)
      Fukuda, S.
    • Journal Title

      Gut Microbes

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate2011

    • Author(s)
      Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clarke, J. M., Topping, D. L., Suzuki, T., Taylor, T. D., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., and Ohno, H
    • Journal Title

      Nature

      Volume: 469 Pages: 543-547

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A dynamic omics approach identifies nutrition-mediated microbial interactions2011

    • Author(s)
      Nakanishi, Y., Fukuda, S., Chikayama, E., Kimura, Y., Ohno, H., and Kikuchi, J
    • Journal Title

      J. Proteome Res

      Volume: 10 Pages: 824-836

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of a mouse ligated Peyer's patch intestinal loop assay to evaluate bacterial uptake by M cells2011

    • Author(s)
      Fukuda, S., Hase, K., and Ohno, H
    • Journal Title

      J. Vis. Exp

      Volume: 58

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice2011

    • Author(s)
      Takahashi, D., Hase, K., Kimura, S., Nakatsu, F., Ohmae, M., Mandai, Y., Sato, T., Date, Y., Ebisawa, M., Kato, T., Obata, Y., Fukuda, S., Kawamura, Y., Dohi, T., Katsuno, T., Yokosuka, O., Waguri, S., and Ohno, H.
    • Journal Title

      Gastroenterology

      Volume: 141 Pages: 621-632

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation2011

    • Author(s)
      Prakash, T., Oshima, K., Morita, H., Fukuda, S., Imaoka, A., Kumar, N., Sharma, V. K., Takahashi, M., Saitou, N., Taylor, T. D., Ohno, H., Umesaki, Y., and Hattori, M.
    • Journal Title

      Cell Host Microbe

      Volume: 10 Pages: 273-284

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bifidobacterium kashiwanohaense sp. nov., isolated from healthy infant faeces2011

    • Author(s)
      Morita, H., Nakano, A., Onoda, H., Toh, H., Oshima, K., Takami, H., Murakami, M., Fukuda, S., Takizawa, T., Kuwahara, T., Ohno, H., Tanabe S., and Hattori, M.
    • Journal Title

      Int. J. Syst. Evol.Microbiol

      Volume: 61 Pages: 2610-2615

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ビフィズス菌が産生する酢酸が病原性大腸菌感染を予防する:糖トランスポーターの重要性2011

    • Author(s)
      福田真嗣
    • Journal Title

      科研費NEWS

      Volume: 1 Pages: 17-17

    • Related Report
      2011 Final Research Report
  • [Journal Article] 食品と腸内細菌叢2011

    • Author(s)
      福田真嗣、大野博司
    • Journal Title

      臨床検査

      Volume: 55 Pages: 183-187

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] ビフィズス菌の産生する酢酸が病原性大腸菌感染を予防する-糖トランスポーターの重要性-2011

    • Author(s)
      福田真嗣、服部正平、大野博司
    • Journal Title

      実験医学

      Volume: 29 Pages: 923-926

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Application of a mouse ligated Peyer's patch intestinal loop assay to evaluate bacterial uptake by M cells2011

    • Author(s)
      Fukuda S., Hase K., et al
    • Journal Title

      J.Vis.Exp.

      Volume: Dec17 Issue: 58

    • DOI

      10.3791/3225

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a monitoring approach for metabolic dynamics in microbial ecosystems by stable isotope labeling technologies2010

    • Author(s)
      Date, Y., Nakanishi, Y., Fukuda, S., Kato, T., Tsuneda, S., Ohno, H., and Kikuchi, J.
    • Journal Title

      J. Biosci. Bioeng

      Volume: 110 Pages: 87-93

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 腸管出血性大腸菌O157H7投与マウスモデルを用いた宿主-腸内フローラ間相互作用の解析2010

    • Author(s)
      福田真嗣、大野博司
    • Journal Title

      日本臨床腸内微生物学会誌

      Volume: 12 Pages: 35-40

    • NAID

      10024187235

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection2012

    • Author(s)
      Fukuda, S.
    • Organizer
      The 4th Stage Surface Barrier Immunology Study Group (SBARIS) 3rd Meeting
    • Place of Presentation
      Osaka(招待講演)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bifidobacteria can protect from enteropathogenic infection through production of acetate2012

    • Author(s)
      Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H.
    • Organizer
      Keystone Symposia
    • Place of Presentation
      The Microbiome, Keystone, Colorado
    • Year and Date
      2012-03-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection2012

    • Author(s)
      Fukuda, S
    • Organizer
      The 4th Stage Surface Barrier Immunology Study Group(SBARIS) 3rd Meeting
    • Place of Presentation
      Osaka
    • Related Report
      2011 Final Research Report
  • [Presentation] Acetate producing bifidobacteria equipped with 'probiotic transporters' protects the host against enteropathogenic infection2011

    • Author(s)
      Fukuda, S. and Ohno, H
    • Organizer
      International symposium, the 40^<th> annual meeting of the Japanese society for Immunology
    • Place of Presentation
      Chiba
    • Year and Date
      2011-11-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Acetate-producing bifidobacteria equipped with 'probiotic transporters' protects the host against enteropathogenic infection2011

    • Author(s)
      Fukuda, S.
    • Organizer
      International symposium, the 40^<th> annual meeting of the Japanese society for Immunology
    • Place of Presentation
      Chiba(招待講演)
    • Year and Date
      2011-11-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Application of statistical correlation method for understanding complex gut ecosystem2011

    • Author(s)
      Fukuda, S.
    • Organizer
      The International Symposium on Nuclear Magnetic Resonance 2011 (ISNMR 2011)
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-11-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Application of statistical correlation method for understanding complex gut ecosystem2011

    • Author(s)
      Fukuda, S., Nakanishi, Y., Date, Y., Ohno, H., Kikuchi, J
    • Organizer
      The International Symposium on Nuclear Magnetic Resonance 2011(ISNMR 2011)
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Bifidobacteria can protect from enteropathogenic infection through production of acetate2011

    • Author(s)
      Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
    • Organizer
      Keystone Symposia : Malnutrition, Gut-Microbial Interactions and Mucosal Immunity to Vaccines
    • Place of Presentation
      New Delhi
    • Year and Date
      2011-11-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Bifidobacteria can protect from enteropathogenic infection through production of acetate2011

    • Author(s)
      Fukuda, S.
    • Organizer
      Keystone Symposia : Malnutrition, Gut-Microbial Interactions and Mucosal Immunity to Vaccines
    • Place of Presentation
      New Delhi, India
    • Year and Date
      2011-11-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] マルチオーミクス解析から見えてきた腸内エコシステムの様相2011

    • Author(s)
      福田真嗣
    • Organizer
      第6回メタボロームシンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2011-10-13
    • Related Report
      2011 Final Research Report
  • [Presentation] マルチオーミクス解析から見えてきた腸内エコシステムの様相2011

    • Author(s)
      福田真嗣
    • Organizer
      第6回メタボロームシンポジウム
    • Place of Presentation
      大阪(招待講演)
    • Year and Date
      2011-10-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] マルチオーミクス解析技術が解き明かす腸内エコシステムの様相2011

    • Author(s)
      福田真嗣
    • Organizer
      2011年度乳酸菌・腸内細菌工学研究部会講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-28
    • Related Report
      2011 Final Research Report
  • [Presentation] マルチオーミクス解析技術が解き明かす腸内エコシステムの様相2011

    • Author(s)
      福田真嗣
    • Organizer
      2011年度乳酸菌・腸内細菌工学研究部会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] マルチオーミクス解析技術を用いた腸内共生システムの理解2011

    • Author(s)
      福田真嗣
    • Organizer
      筑波大学革新的研究支援プログラム「微生物バイオ資源の新領域開拓と利用」第6回セミナー
    • Place of Presentation
      筑波(招待講演)
    • Year and Date
      2011-08-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection2011

    • Author(s)
      Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
    • Organizer
      15th International Congress of Mucosal Immunology
    • Place of Presentation
      Paris
    • Year and Date
      2011-07-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection2011

    • Author(s)
      Fukuda, S.
    • Organizer
      15th International Congress of Mucosal Immunology
    • Place of Presentation
      Paris, France
    • Year and Date
      2011-07-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Carbohydrate transporters confer a probiotic effect to bifidobacteria2011

    • Author(s)
      Fukuda, S
    • Organizer
      The International Scientific Conference on Probiotics and Prebiotics 2011
    • Place of Presentation
      Kosice, Slovakia
    • Year and Date
      2011-06-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Carbohydrate transporters confer a probiotic effect to bifidobacteria2011

    • Author(s)
      Fukuda, S.
    • Organizer
      The International Scientific Conference on Probiotics and Prebiotics 2011
    • Place of Presentation
      Kosice, Slovakia(招待講演)
    • Year and Date
      2011-06-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] マルチオーミクス手法を用いた宿主-腸内フローラ間相互作用の解析2011

    • Author(s)
      福田真嗣
    • Organizer
      東京大学グローバルCOEプログラム「ゲノム情報ビッグバンから読み解く生命圏」第16回GCOE談話会
    • Place of Presentation
      柏(招待講演)
    • Year and Date
      2011-04-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸管出血性大腸菌O157 : H7感染死マウスモデルを用いたビフィズス菌による感染死抑止機構の解明2011

    • Author(s)
      福田真嗣、藤英博、長谷耕二、大島健志朗、中西裕美子、吉村和敏、戸邉亨、鈴木徹、伊藤喜久治、菊地淳、森田英利、服部正平、大野博司
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 腸管出血性大腸菌O157:H7感染死マウスモデルを用いたビフィズス菌による感染死抑止機構の解明2011

    • Author(s)
      福田真嗣
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Integrated omits approach identified acetate produced by probiotic bifid obacteria to protect host from enteropathogenic infection2011

    • Author(s)
      Fukuda, S
    • Organizer
      The 4th Stage Surface Barrier Immunology Study Group (SBARIS) 3rd Meeting
    • Place of Presentation
      東京
    • Year and Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multiple omics identified probiotic bacterial metabolites to prevent gut inflammation provoked by enterohemorrhagic Escherichia coli O157 : H7 infection2010

    • Author(s)
      Fukuda、S., Toh, H., Nakanishi, Y., Hase, K., Oshima, K., Yoshimura, K., Tobe, T., Taylor, T. D., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe
    • Year and Date
      2010-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Multiple omics identified probiotic bacterial metabolites to prevent gut inflammation provoked by enterohemorrhagic Escherichia coli O157:H7 infection2010

    • Author(s)
      Fukuda, S
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Elucidation of the mechanisms of host-microbial crosstalk in the gut2010

    • Author(s)
      Fukuda, S
    • Organizer
      Genomic Sciences Research Complex(GSC)七夕ミーティング
    • Place of Presentation
      横浜
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Book] 微生物機能学第5章代謝経路を人工的に修飾して物質を効率よく生産する-代謝制御発酵-2012

    • Author(s)
      福田真嗣
    • Publisher
      三共出版
    • Related Report
      2011 Final Research Report
  • [Book] 微生物機能学2012

    • Author(s)
      福田真嗣
    • Publisher
      三共出版(印刷中)
    • Related Report
      2011 Annual Research Report
  • [Book] 世紀を超えるビフィズス菌の研究~その基礎と臨床応用から製品開発へ~第2章2部ビフィズス菌定着マウスのマルチオーミクス解析2011

    • Author(s)
      福田真嗣
    • Publisher
      財団法人日本ビフィズス菌センター
    • Related Report
      2011 Final Research Report
  • [Book] 世紀を超えるビフィズス菌の研究~その基礎と臨床応用から製品開発へ~2011

    • Author(s)
      福田真嗣
    • Publisher
      財団法人日本ビフィズス菌センター(印刷中)
    • Related Report
      2010 Annual Research Report
  • [Book] 難培養微生物研究の最新技術II~ゲノム解析を中心とした最前線と将来展望~第14章微生物群集のメタボローム統合化解析2010

    • Author(s)
      福田真嗣
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] メタゲノム解析技術の最前線2010

    • Author(s)
      福田真嗣
    • Total Pages
      231
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Book] 難培養微生物研究の最新技術II~ゲノム解析を中心とした最前線と将来展望~2010

    • Author(s)
      福田真嗣
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Remarks] 福田真嗣、大野博司ビフィズス菌が産生する酢酸による病原性大腸菌感染症の予防、大学共同利用機関法人情報・システム研究機構ライフサイエンス統合データベースセンターライフサイエンス新着論文レビュー(2011)

    • URL

      http://first.lifesciencedb.jp/archives/2284#more-2284

    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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