Development of vaccine against Oncorhynchus masou virus(OMV) disease based on whole genome analysis of OMV

• Project/Area Number: 22780168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: KASAI Hisae 北海道大学, 大学院・水産科学研究院, 准教授 (50399995)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 魚病 / 増養殖 / 魚類ウイルス / 癌ウイルス / ワクチン / ゲノムワイド解析

Research Abstract

Oncorhynchus masouvirus(OMV) disease is a hepatitic, oncogenic and skin ulcerative condition of national and international socio-economic importance. The OMV genome sequences of three strains including the high virulent strain against rainbow trout revealed about 160 kbp genome composed of a unique long sequence, and a unique short fragment flanked by inverted repeats. ORFs involved in pathogenicity or oncogenicity were found in OMV genome. Furthermore an effective vaccine was developed in which relative percent survival(RPS) reached 74%.

Research Products

• [Journal Article] Inactivation of koi-herpesvirus(KHV) in water using bacteria isolated from carp intestines and carp habitats (2012)
  • Author(s): Yoshida, N., R. Sasaki, H. Kasai and M. Yoshimizu
  • Journal Title: Journalof Fish Diseases Volume: 35(in press)
  • Peer Reviewed
• [Journal Article] Inactivation of koi-herpesvirus (KHV) in water using bacteria isolated from carp intestines and carp habitats (2012)
  • Author(s): Yoshida, N., et al.
  • Journal Title: Journal of Fish Diseases Volume: 35(in press)
  • Peer Reviewed
• [Journal Article] Elimination of Escherichia coli from oysters using electrolyzed seawater (2011)
  • Author(s): Kasai, H., et al
  • Journal Title: Aquaculture Volume: 319 Issue: 3-4 Pages: 315-318
  • DOI: 10.1016/j.aquaculture.2011.07.025
  • Peer Reviewed
• [Journal Article] Absorbability of bacteria isolated from active sludge of fish rearing waste water treatment facilities (2011)
  • Author(s): 吉田夏子, 他
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 77 Issue: 1 Pages: 94-96
  • DOI: 10.2331/suisan.77.94
  • NAID: 10027411727
  • ISSN: 0021-5392, 1349-998X
  • Peer Reviewed
• [Presentation] サケ科魚ヘルペスウイルスOncorhynchus masou virus (OMV)の全ゲノム解析 (2012)
  • Author(s): 森真希, 他
  • Organizer: 平成24年度日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2012-03-17
• [Presentation] サケ科魚ヘルペスウイルスOncorhynchus masou virus(OMV)の全ゲノム解析 (2012)
  • Author(s): 森真希・笠井久会・吉水守
  • Organizer: 平成24年度日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] サケ科魚類のヘルペスウイルス(OMV)不活化ワクチンに必要な抗原量 (2011)
  • Author(s): 笠井久会・降幡充・吉水守
  • Organizer: 日本魚病学会
  • Place of Presentation: 東京大学(東京都文京区)
  • Year and Date: 2011-03-27
• [Presentation] Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed production facility, and net pen or tank in Japan (2011)
  • Author(s): Yoshimizu, M. and H. Kasai
  • Organizer: 3rd International Symposium on Cage Aquaculture in Asia
  • Place of Presentation: Malaysia
• [Presentation] Survivability of koi herpesvirus(KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria (2011)
  • Author(s): Yoshida, N., H. Kasai and M. Yoshimizu
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Hokkaido, Japan
• [Presentation] サケ科魚類のヘルペスウイルス(OMV)不活化ワクチンに必要な抗原量 (2011)
  • Author(s): 笠井久会・降幡充・吉水守
  • Organizer: 平成23年度日本魚病学会春季大会
  • Place of Presentation: 東京大学
• [Presentation] 抗KHV物質産生細菌による魚類飼育排水中のKHVの不活化 (2011)
  • Author(s): 吉田夏子・笠井久会・吉水守
  • Organizer: 平成23年度日本魚病学会春季大会
  • Place of Presentation: 東京大学
• [Presentation] KHVの環境水中における生存性および抗KHV活性を有する細菌の探索 (2011)
  • Author(s): 吉田夏子・清水智子・笠井久会・吉水守
  • Organizer: 平成23年度日本魚病学会春季大会
  • Place of Presentation: 東京大学
• [Presentation] Survivability of koi Herpesvirus (KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria (2011)
  • Author(s): Yoshida, N., et al.
  • Organizer: International Union of Microbiological Society 2011 Sapporo Congress
  • Place of Presentation: 札幌コンベンションホール(北海道)
• [Presentation] Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed production facility, and net-pen or tank in Japan (2011)
  • Author(s): Yoshimizu, M., Kasai, H.
  • Organizer: Cage Aquaculture in Asia 3
  • Place of Presentation: Putra World Trade Center(マレーシア)
• [Presentation] 洗卵による病原細菌・ウイルスの除去効果および卵内への侵入防止効果 (2010)
  • Author(s): 吉水守・笠井久会・小原昌和
  • Organizer: 第4回サケ学研究会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-12-18
• [Presentation] 洗卵による病原細菌・ウイルスの除去効果および卵内への侵入防止効果 (2010)
  • Author(s): 吉水守・笠井久会・小原昌和
  • Organizer: サケ学研究会
  • Place of Presentation: 北海道大学(函館市)
  • Year and Date: 2010-12-18
• [Presentation] 細胞の増殖およびCPE形成時の形態変化 (2010)
  • Author(s): 森真希, 笠井久会, 吉水守
  • Organizer: 日本魚病学会
  • Place of Presentation: 三重大学(津市)
  • Year and Date: 2010-09-22
• [Presentation] 等調液洗卵法による病原細菌およびウイルスの除去と卵内感染防除効果 (2010)
  • Author(s): 小原昌和・小川滋・笠井久会・吉水守
  • Organizer: 日本魚病学会
  • Place of Presentation: 三重大学(津市)
  • Year and Date: 2010-09-22
• [Presentation] Occurrence of Intra-Ovum Infection and Effect of Egg-washing with Isotonic Solution on Elimination of Bacteria and Viruses before Fertilization in Salmon Aquaculture (2010)
  • Author(s): Kohara, M., S.Ogawa, H.Kasai, M.Yoshimizu
  • Organizer: 6^<th> International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, Florida, U.S.A
  • Year and Date: 2010-09-06
• [Presentation] 等調液洗卵法による病原細菌およびウイルスの除去と卵内感染防除効果 (2010)
  • Author(s): 小原昌和・小川滋・笠井久会・吉水守
  • Organizer: 平成22年度日本魚病学会大会
  • Place of Presentation: 三重大学
• [Presentation] 魚類病原細菌および一般細菌の人工感染試験による卵内感染機序の検討 (2010)
  • Author(s): 小原昌和・笠井久会・吉水守
  • Organizer: 平成22年度日本魚病学会大会
  • Place of Presentation: 三重大学
• [Presentation] 細胞の増殖およびCPE形成時の形態変化 (2010)
  • Author(s): 森真希・笠井久会・吉水守
  • Organizer: 平成22年度日本魚病学会大会
  • Place of Presentation: 三重大学
• [Presentation] Occurrence of Intra-Ovum Infection and Effect of Egg-washing with Isotonic Solution on Elimination of Bacteria and Viruses before Fertilization in Salmon Aquaculture (2010)
  • Author(s): Kohara, M., S. Ogawa, H. Kasai and M. Yoshimizu
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: U. S. A.
• [Presentation] Survivability of koi herpesvirus (KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria (2010)
  • Author(s): Yoshida, N., H.Kasai, M.Yoshimizu
  • Organizer: 6^<th> International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, Florida, U.S.A
• [Book] Fish Diseases and Disorders (2011)
  • Author(s): Yoshimizu, M. and H. Kasai
  • Publisher: CABI International
• [Book] Chapter 7 ; Oncogenic viruses, 『2nd edition of Fish Diseases & Disorders, Vol.3』 (2011)
  • Author(s): Yoshimizu, M., Kasai, H.
  • Publisher: CABI International
• [Book] Chapter 7 ; Oncogenic viruses, 『Fish Diseases and Disorders, Vol.3 : Viral, Bacterial and Fungal Infections (2^<nd> edition)』 (2011)
  • Author(s): 吉水守, 笠井久会(共著)
  • Publisher: CABI International, Oxford, England