Molecular and developmental genetics of metamorphosis in fish

• Project/Area Number: 22780170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Tohoku University
• Principal Investigator: YOKOI Hayato 東北大学, 大学院・農学研究科, 助教 (40569729)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 変態 / 発生 / 小型魚類 / モデル生物

Research Abstract

This study aimed to accumulate the basic scientific knowledge on the metamorphosis in fish to help establishing more efficient production of the flounder aquaculture which has a huge economical impact in Japan. I conducted molecular and developmental genetic studies using medaka as a model. GFP transgenic lines were generated in order to monitor in vivo thyroid hormone level in a live fish. In another approach to establish medaka as a model system for studying metamorphosis, approximately 20 candidate marker genes that are implicated in thyroid grand development and/or thyroid hormone signaling were cloned and their embryonic expression patterns were examined.

Research Products

• [Journal Article] Continuous illumination through larval development suppresses dopamine synthesis in the suprachiasmatic nucleus, causing activation of α-MSH synthesis in the pituitary and abnormal metamorphic skin pigmentation in flounder. (2012)
  • Author(s): 伊藤香絵, 鷲尾洋平, 藤浪祐一郎, 清水大輔, 横井勇人, 宇治督, 鈴木徹
  • Journal Title: General and Comparative Endocrinology Volume: 176 Issue: 2 Pages: 215-221
  • DOI: 10.1016/j.ygcen.2012.01.017
  • Peer Reviewed
• [Journal Article] Detection of vitellogenin incorporation into zebafish oocytes by FITC (2011)
  • Author(s): 松田陽平、伊藤淑恵、横井勇人、橋本寿史、鈴木徹
  • Journal Title: Reproductive Biology and Endocrinology Volume: 9巻 Issue: 1 Pages: 45-45
  • DOI: 10.1186/1477-7827-9-45
  • Peer Reviewed
• [Journal Article] Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence. (2011)
  • Author(s): Matsuda Y, Ito Y, Hashimoto H, Yokoi H, Suzuki T.
  • Journal Title: Reproductive Biology and Endocrinology Volume: 9 Pages: 45-45
  • Peer Reviewed
• [Journal Article] Evolution of developmental regulation in the vertebrate FgfD subfamily (2010)
  • Author(s): Jovelin R, Yan YL, He X, Catchen J, Amores A, Canestro C, Yokoi H and Postlethwait JH
  • Journal Title: J Exp Zool B Mol Dev Evol Volume: 314(1) Issue: 1 Pages: 33-56
  • DOI: 10.1002/jez.b.21307
  • Peer Reviewed
• [Journal Article] Evolution of developmental regulation in the vertebrate FgfD subfamily. (2010)
  • Author(s): Jovelin, R., Yan, Y.L., He, X., Catchen, J., Amores A., Canestro, C., Yokoi H., Postlethwait, J.H.
  • Journal Title: J.Exp.Zool.B : Mol.Dev.Evol. Volume: 314B Pages: 33-56
  • Peer Reviewed
• [Presentation] 魚類におけるゲノム倍加と機能分配 (2012)
  • Author(s): 横井勇人
  • Organizer: アクアゲノム研究会
  • Place of Presentation: 長崎大学(長崎市)(招待講演)
  • Year and Date: 2012-10-01
• [Presentation] メダカにおける甲状腺関連マーカー遺伝子の発現による甲状腺正常発生の観察 (2012)
  • Author(s): 阿部雄二、鈴木徹、横井勇人
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] ヒラメ仔魚で左右非対称に発現する遺伝子の単離の試み (2012)
  • Author(s): 横井勇人、近藤大地、藤浪祐一朗、宇治督、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] 仔魚期の24時間照明飼育で起こる視床下部・下垂体経路の異常 (2012)
  • Author(s): 伊藤香絵、鷲尾洋平、清水大輔、藤浪祐一郎、宇治督、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] ヒラメ仔魚で左右非対称に発現する遺伝子の単離の試み (2012)
  • Author(s): 横井勇人、近藤、藤浪、宇治、鈴木
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2012-03-27
• [Presentation] メダカにおける甲状腺関連マーカー遺伝子の発現による甲状腺正常発生の観察 (2012)
  • Author(s): 阿部雄二、鈴木徹、横井勇人
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2012-03-27
• [Presentation] Circadian pacemaker in the suprachiasmatic nuclei of teleost fish revealed by rhythmic per2 expression (2012)
  • Author(s): Tohru Suzuki, Nanako Watanabe, Hayato Yokoi
  • Organizer: International Symposium : Frontiers in Behavioral Brain Science ~ Solving the mystery of sleep~
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2012-03-20
• [Presentation] 魚類におけるゲノム倍加と機能分配 (2011)
  • Author(s): 横井勇人
  • Organizer: アクアゲノム研究会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-10-01
• [Presentation] A trial to dissect the flounder"hentai": isolation of genes expressed asymmetrically in left or right side in flounder (2011)
  • Author(s): Hayato Yokoi, Daichi Kondo, Yuichiro Fujinami, Tohru Suzuki
  • Organizer: 17th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 東レ研修センター(三島市)
  • Year and Date: 2011-09-08
• [Presentation] A trial to dissect the flounder "hentai" : isolation of genes expressed asymmetrically in left or right side in flounder (2011)
  • Author(s): Hayato Yokoi, Daichi Kondo, Yuichiro Fujinami, Tohru Suzuki
  • Organizer: 17th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 東レ研修センター(三島市)
  • Year and Date: 2011-09-08
• [Presentation] Isolation of genes enriched asymmetrically in left or right side in flounder (2011)
  • Author(s): Hayato Yokoi, Daichi Kondo, Xiaoming Wu, Yuichiro Fujinami, Tohru Suzuki
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] Left-sided expression of pitx2 at dorsal diencephalon and gut is maintained throughout larval development in flounders for metamorphic morphogenesis (2011)
  • Author(s): Daichi Kondo, Yuichiro Fujinami, Susumu Uji, Hisashi Hashimoto, Hayato Yokoi, Tohru Suzuki
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] Isolation of genes enriched asymmetrically in left or right side in flounder (2011)
  • Author(s): Hayato Yokoi, D Kondo, X Wu, Y Fujinami, T Suzuki
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] oct4-GFPトランスジェニックゼブラフィシュによる細胞の多能性の可視化 (2010)
  • Author(s): 阿部光太、横井勇人、橋本寿史、鈴木徹
  • Organizer: 平成22年度日本水産学会東北支部会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-11-06
• [Presentation] Evolution of soxE genes in teleost fish revealed by comparative expression analysis and genomics (2010)
  • Author(s): Hayato Yokoi, Yi-Lin Yan, Ayano Miyagi, Mark Currey, Julian Catchen, William Cresko, Tohru Suzuki, John Postlethwait
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 京都国際会館
  • Year and Date: 2010-06-21
• [Presentation] Evolution of soxE genes in teleost fish revealed by comparative expression analysis and genomics. (2010)
  • Author(s): Yokoi, H.・Yan, Y.-L.・Miyagi, A.・Currey, M.C.・Catchen, J.M.・Cresko, W.A.・Suzuki, T.・Postlethwait, J.H.
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 京都市
• [Book] Genome duplication and subfunction partitioning : Sox9 in medaka and other vertebrates. In"Medaka : Model for Organogenesis, Human Disease and Evolution." (2011)
  • Author(s): Yokoi H and Postlethwait JH, Takeda H, Naruse K, Tanaka M
  • Publisher: Springer
• [Book] Genome duplication and subfunction partitioning : Sox9 in medaka and other vertebrates (2011)
  • Author(s): Hayato Yokoi, John H.Postlethwait
  • Publisher: Springer
• [Book] Medaka : Model for Organogenesis, Human Disease and Evolution. (2010)
  • Author(s): Hayato Yokoi, John Postlethwait
  • Publisher: Springer-Verlag
• [Remarks] 東北大学研究者紹介
  • URL: http://db.tohoku.ac.jp/whois/detail/0b42e27653b7f35e08dfd12bc8aceb84.html