Role of cellular and humoral immunity against viral-infection in teleost fish

• Project/Area Number: 22780176
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: SOMAMOTO Tomonori 九州大学, 農学研究院, 准教授 (40403993)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 魚類 / 免疫学 / ウイルス学 / 獲得免疫 / ギンブナ / 水産学 / ウイルス / T細胞 / 細胞性免疫

Research Abstract

To understand a role of cellular and humoral immunity against viral-infection in teleost fish, we have investigated in vivo functions of T-cells and B-cells in controlling of viral infection employing clonal ginbuna crucian carp. The results obtained in this study suggest that anti-viral T-cell functions in teleosts do not completely correspond to mammalian ones.

Research Products

• [Journal Article] Identification of anti-viral cytotoxic effector cells in the ginbuna crucian carp Carassius auratus langsdorfii. (2013)
  • Author(s): Somamoto T, Nakanishi T and Nakao M.
  • Journal Title: Dev. Comp. Immunol. Volume: 39 Issue: 4 Pages: 370-377
  • DOI: 10.1016/j.dci.2012.11.001
  • Peer Reviewed
• [Journal Article] Diversity of CD2 subfamily receptors in cyprinid fishes. (2012)
  • Author(s): Sameshima S, Nakao M, Somamoto T.
  • Journal Title: Result Immunol Volume: 2 Pages: 25-34
  • DOI: 10.1016/j.rinim.2012.01.003
  • Peer Reviewed
• [Journal Article] The binding spectra of carp C3 isotypes against natural targets independent of the bindingspecificity of their thioester (2012)
  • Author(s): Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M.
  • Journal Title: Dev.Comp. Immunol. Volume: 38(1) Issue: 1 Pages: 10-16
  • DOI: 10.1016/j.dci.2012.03.004
  • Peer Reviewed
• [Journal Article] Cytotoxic T cells in teleost fish (2011)
  • Author(s): Nakanishi, T., H.Toda, Y.Shibasaki, T.Somamoto
  • Journal Title: Dev.Comp.Immunol. Volume: 35 Issue: 12 Pages: 1317-1323
  • DOI: 10.1016/j.dci.2011.03.033
  • Peer Reviewed
• [Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish (2011)
  • Author(s): Toda,H., Saito,Y., Koike,T., Takizawa,F., Araki,K., Yabu,T., Somamoto,T., Suetake,H., Suzuki,Y., Ototake,M., Moritomo,T., and Nakanishi,T.
  • Journal Title: Dev. Comp. Immunol Volume: 35 Issue: 6 Pages: 650-660
  • DOI: 10.1016/j.dci.2011.01.013
  • Peer Reviewed
• [Journal Article] The complement system in teleost fish : Progress of post-homolog-hunting researches (2011)
  • Author(s): Nakao M, Tsujikura M, Ichiki S, Tam K.Vo, Somamoto T.
  • Journal Title: Dev.Comp.Immunol. Volume: 35 Issue: 12 Pages: 1296-1308
  • DOI: 10.1016/j.dci.2011.03.003
  • Peer Reviewed
• [Journal Article] Molecular characterization of MHC class I and beta-2 microglobulin in a clonal strain of ginbuna crucian carp, Carassius auratus langsdorfii (2011)
  • Author(s): Urabe S, Somamoto T, Sameshima S, Unoki-Kato Y, Nakanishi T, Nakao M
  • Journal Title: Fish Shellfish Immunol. Volume: 31 Issue: 3 Pages: 469-474
  • DOI: 10.1016/j.fsi.2011.06.004
  • Peer Reviewed
• [Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish (2011)
  • Author(s): Toda H., Y.Saito, T.Koike, F.Takizawa, K.Araki, T.Yabu, T.Somamoto, H.Suetake, Y.Suzuki, M.Ototake, T.Moritomo, T.Nakanishi
  • Journal Title: Dev.Comp.Immunol. Volume: 35 Pages: 650-660
  • Peer Reviewed
• [Journal Article] The complement system in teleost fish : Progress of post-homolog-hunting researches (2011)
  • Author(s): Miki Nakao, Masakazu Tsujikura, Satoko Ichiki, Tam K.Vo, Tomonori Somamoto
  • Journal Title: Dev.Comp.Immunol. Volume: 35 Pages: 1296-12308
  • Peer Reviewed
• [Journal Article] 魚類における膜型補体制御因子の同定と機能解析:次世代魚病ワクチンアジュバントへの展開に向けて (2010)
  • Author(s): 辻倉正和・杣本智軌・鵜木陽子・中尾実樹
  • Journal Title: 生物機能研究 Volume: 14巻 Pages: 38-44
• [Presentation] ギンブナ樹状細胞マーカー CD83 に対する抗体の作製と発現 (2013)
  • Author(s): 豊福太樹, 山下基子, 長沢貴宏, 杣本智軌, 中尾実樹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京都、東京海洋大学
  • Year and Date: 2013-03-26
• [Presentation] コイ栓球は他の白血球により活性化され貪食能を示す (2013)
  • Author(s): 長沢貴宏, 中易千早, 杣本智軌, 中尾実樹
  • Organizer: 本水産学会春季大会
  • Place of Presentation: 東京都、東京海洋大学
  • Year and Date: 2013-03-26
• [Presentation] ギンブナにおけるコイヘルペスウイルスに対する防御機構の解明 (2013)
  • Author(s): 福田圭佑・杣本智軌・中尾実樹
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 福岡市、九州大学
  • Year and Date: 2013-01-26
• [Presentation] Rag1 欠損ゼブラフィッシュにおけるT 細胞の機能不全の検証 (2013)
  • Author(s): 白水正道・杣本智軌・吉浦康寿・中尾実樹
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 福岡市、九州大学
  • Year and Date: 2013-01-26
• [Presentation] コイ膜型補体制御因子 cTecrem のモノクローナル抗体作製と機能解析 (2013)
  • Author(s): 中村亮太・辻倉正和・中尾実樹・杣本智軌
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 福岡市、九州大学
  • Year and Date: 2013-01-26
• [Presentation] Rag1欠損ゼブラフィッシュにおけるT細胞の機能不全の検証 (2013)
  • Author(s): 白水正道・杣本智軌・吉浦康寿・中尾実樹
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 福岡市、九州大学
• [Presentation] コイ栓球は他の白血球により活性化され貪食能を示す (2013)
  • Author(s): 長沢貴宏, 中易千早, 杣本 智軌, 中尾 実樹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京都、東京海洋大学
• [Presentation] Streptococcus parauberisに対するヒラメ白血球の活性化 (2012)
  • Author(s): 塚本春香・杣本智軌・中尾実樹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2012-03-28
• [Presentation] 魚類の細胞性免疫機構について (2012)
  • Author(s): 中西照幸・杣木智軌・柴崎康宏・藪健史
  • Organizer: 第153回日本獣医学会微生物分科会シンポジウム
  • Place of Presentation: 大宮ソニックシティー(埼玉県)
  • Year and Date: 2012-03-27
• [Presentation] 不活化ウイルス経腸管感作後のギンブナ T 細胞の免疫応答 (2012)
  • Author(s): 多治見誠亮・杣本智軌・中西照幸・中尾実樹
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 鹿児島大学 (鹿児島県)
  • Year and Date: 2012-01-19
• [Presentation] 不活化ウイルス経腸管感作後のギンブナT細胞の免疫応答 (2012)
  • Author(s): 多治見誠亮・杣本智軌・中西照幸・中尾実樹
  • Organizer: 日本水産学会九州支部大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2012-01-19
• [Presentation] Functions of CD8-positive and CD4-positive lymphocytes against virus-infectionin ginbuna crucain carp (2012)
  • Author(s): Tomonori Somamoto, Teruyuki Nakanishi, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and Comparative Immunolog
  • Place of Presentation: Fukuoka city, Hilton Sea Hawk Hote
• [Presentation] Isotypic Diversity in the Ontogenetic Expression of the Complement Componentin the Common Carp(Cyprinus carpio) (2012)
  • Author(s): Vo Kha Tam, Chie Okura, Masakazu Kondo,Tomonori Somamoto, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka city, Hilton Sea Hawk Hotel
• [Presentation] Phagocytosis and bactericidal abilities of teleost thrombocytes (2012)
  • Author(s): Takahiro Nagasawa, Chihaya Nakayasu,Tomomasa Matsuyama, Aja M. Rieger, Daniel R. Barreda, Tomonori Somamoto, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and ComparativeImmunology
  • Place of Presentation: Fukuoka city, Hilton Sea Hawk Hotel
• [Presentation] Functions of CD8-positive and CD4-positive lymphocytes against virus-infection in ginbuna crucain carp (2012)
  • Author(s): Tomonori Somamoto, Teruyuki Nakanishi, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka city, Hilton Fukuoka Sea Hawk Hotel
• [Presentation] Isotypic Diversity in the Ontogenetic Expression of the Complement Componentin the Common Carp (Cyprinus carpio) (2012)
  • Author(s): Vo Kha Tam, Chie Okura, Masakazu Kondo, Tomonori Somamoto, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka city, Hilton Fukuoka Sea Hawk Hotel
• [Presentation] Phagocytosis and bactericidal abilities of teleost thrombocytes (2012)
  • Author(s): Takahiro Nagasawa, Chihaya Nakayasu, Tomomasa Matsuyama, Aja M. Rieger, Daniel R. Barreda, Tomonori Somamoto, Miki Nakao
  • Organizer: 12th Congress of International Society of Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka city, Hilton Fukuoka Sea Hawk Hotel
• [Presentation] Rag1 欠損ゼブラフィッシュにおける獲得免疫不全の検証 (2011)
  • Author(s): 白水 正道・徳永 弓枝・吉浦 康寿、乙竹充・杣本 智軌・中尾 実樹
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 独立行政法人海洋研究開発機構(神奈川県)
  • Year and Date: 2011-08-21
• [Presentation] Rag1欠損ゼブラフィッシュにおける獲得免疫不全の検証 (2011)
  • Author(s): 白水正道・徳永弓枝・吉浦康寿、乙竹充・杣本智軌・中尾実樹
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 独立行政法人海洋研究開発機構(神奈川県)
  • Year and Date: 2011-08-21
• [Presentation] 魚類の細胞性免疫機構について (2011)
  • Author(s): 中西照幸、杣本智軌、戸田秀明、柴崎康宏、藪健史
  • Organizer: 第151回日本獣医学会 微生物シンポジウム
  • Place of Presentation: 日東京農工大学
  • Year and Date: 2011-03-31
• [Presentation] 魚類の細胞性免疫機構について (2011)
  • Author(s): 中西照幸、杣本智軌、戸田秀明、柴崎康宏、藪健史
  • Organizer: 第151回日本獣医学会微生物シンポジウム2
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2011-03-31
• [Presentation] Function of diversity of the complement component isotypes in bony fish innate immunity: A model study using the common carp (2010)
  • Author(s): Nakao M, Ichiki S, Mutsuro J, Tsujikura M, Somamoto T
  • Organizer: The ninth International Congress on the Biologyof Fish
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-09-10
• [Presentation] Function of diversity of the complement component isotypes in bony fishinnate immunity : A model study using the common carp (2010)
  • Author(s): Nakao M, Ichiki S, Mutsuro J, Tsujikura M, Somamoto T
  • Organizer: The ninth International Congress on the Biology of Fish
  • Place of Presentation: Barcelona, Spain(招待講演)
  • Year and Date: 2010-09-10
• [Presentation] コイ補体レクチン経路に関連するコレクチンの異物認識多様性 (2010)
  • Author(s): 一木昭土、塚本春香、杣本智軌、中尾実樹
  • Organizer: 第47回補体シンポジウム
  • Place of Presentation: コラッセふくしま(福島県)
  • Year and Date: 2010-09-10
• [Presentation] イルス感染で誘導されたギンブナ細胞傷害T 細胞と単球の細胞傷害活性 (2010)
  • Author(s): 杣本 智軌、中西 照幸、中尾 実樹
  • Organizer: 日本比較免疫学会 第22回学術集会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-08-02
• [Presentation] 中尾実樹、魚類における膜型補体制御因子の同定 (2010)
  • Author(s): 辻倉 正和、塚本 春香、杣本智軌、中尾実樹
  • Organizer: 日本比較免疫学会 第22回学術集会、第22回学術集会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-08-02
• [Presentation] Characterization of zymosan-binding proteins in Nile Tilapia serum (2010)
  • Author(s): Soha Gomma, T. Somamoto, M. Nakao
  • Organizer: 日本比較免疫学会 第22回学術集
  • Place of Presentation: 九州大学
  • Year and Date: 2010-08-02
• [Presentation] ウイルス感染で誘導されたギンブナ細胞傷害T細胞と単球の細胞傷害活性 (2010)
  • Author(s): 杣本智軌、中西照幸、中尾実樹
  • Organizer: 日本比較免疫学会第22回学術集会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2010-08-02
• [Presentation] 魚類における膜型補体制御因子の同定 (2010)
  • Author(s): 辻倉正和、塚本春香、杣本智軌、中尾実樹
  • Organizer: 日本比較免疫学会第22回学術集会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2010-08-02
• [Presentation] Characterization of zymosan-binding proteins in Nile Tilapiaserum (2010)
  • Author(s): Soha Gomma, T.Somamoto, M.Nakao
  • Organizer: 日本比較免疫学会第22回学術集会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2010-08-02
• [Book] 補体への招待 「2.5.2. 補体系の進化:脊椎動物」 (大井洋之、木下タロウ、松下操 編) (2011)
  • Author(s): 中尾実樹 杣本智軌
  • Total Pages: 6
  • Publisher: メジカルビュー社
• [Book] 補体への招待「2.5.2.補体系の進化:脊椎動物」(大井洋之、木下タロウ、松下操編) (2011)
  • Author(s): 中尾実樹, 杣本智軌
  • Total Pages: 6
  • Publisher: メジカルビュー社