Protective immunity against intracellularbacteria infish
| Project/Area Number |
22780179
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Kagoshima University |
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Principal Investigator |
ARAKI Kyosuke 鹿児島大学, 水産学部, 助教 (30409073)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 魚病 / ワクチン / 細胞内寄生細菌 / 細胞性免疫 / T細胞 / ブリ / エドワジエラ症 / ミコバクテリウム症 / ブリ属魚類 / ギンブナ / インターフェロンガンマ / クローンギンブナ / 細胞障害性T細胞 / Edwardsiella tarda |
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| Research Abstract |
To elucidate whether cell-mediated immunity and/or humoral immunity contribute to protection against Edwardsiella tardainfection in fish, we examined T-cell related gene expression, specific antibody titers, bacterial clearance, and adoptive transfer of immunized lymphocyte subsets. Our results indicate that cell mediated immunity play an important roles in the protection against E. tardainfection in fish. In addition, cytotoxic T lymphocytes are crucial player in protecting fish from E. tardainfection.Subsequently, we have cloned T-cell related genes from yellowtail. Using these genes as a marker, vaccine efficacy of inactivated vaccine against mycobacteriosis was evaluated. Inactivated vaccine with adjuvant induced cell-mediated immune response, although inactivated cells enhanced humoral immunity in yellowtail.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish2011
Author(s)
Toda,H., Saito,Y., Koike,T., Takizawa,F., Araki,K., Yabu,T., Somamoto,T., Suetake,H., Suzuki,Y., Ototake,M., Moritomo,T., and Nakanishi,T.
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Journal Title
Dev. Comp. Immunol
Volume: 35
Issue: 6
Pages: 650-660
DOI
Related Report
Peer Reviewed
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[Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish.2011
Author(s)
Toda, H., Y.Saito, T.Kioke, F.Takizawa, K.Araki, T.Yabu, T.Somamoto, H.Suetake, Y.Suzuki, M.Ototake, T.Moritomo, T.Nakanishi
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Journal Title
Developmental and Comparative Immunology
Volume: 35
Pages: 650-660
Related Report
Peer Reviewed
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