Population stagnant growth caused by cryptic species of Brachionus plicatilis species complex

• Project/Area Number: 22780182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Kitasato University
• Principal Investigator: YOSHINAGA Tatsuki 北里大学, 海洋生命科学部, 講師 (30406912)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: シオミズツボワムシ / 初期餌料 / 隠蔽種 / 複合種 / 分子系統 / 生活史特性 / 餌料生物 / 種苗生産 / 酸化ストレス耐性 / 低温ストレス耐性 / チトクロームオキシダーゼI / ユグロン / ミトコンドリアDNA

Research Abstract

Rotifer Brachionus plicatilis has been an indispensable zooplankton as a live-food in aquaculture since 1960s. Three types of L, S and SS have been recognized based on their body size, but recent studies revealed that B. plicatilis consists of genetically-isolated species/strains, called species complex. Based on substitutions in the COI locus, the specimens examined in this study were classified into 5 genetically-isolated strains. Subsequently, the two strains of the L-type, Amami and Obama strain, were further investigated to compare their life-history parameters. The Amami strain showed relatively faster growth and maturation, but had lower stavation resistance than the Obama strain. These differences in their life-history suggested that the Amami and Obama strains could be considered to be r-and K-strategists, respectively. Such different adaptive behaviors between the two strains can cause the competition in the mass-culture, and resulted in the stagnant growth or even collapse of the population.

Research Products

• [Journal Article] Genetic identification and morphology of naturally spawned eggs of the Japanese eel Anguilla japonica collected in the western North Pacific (2011)
  • Author(s): Yoshinaga T, Miller MJ, Yokouchi K, Otake T, Kimura S, Aoyama J, Watanabe S, Shinoda A, Oya M, Miyazaki S, Zenimoto K, Sudo R, Takahashi T, Ahn H, Manabe R, Hagihara S, Morioka H, Itakura H, Machida M, Ban K, Shiozaki M, Ai B, Tsukamoto K
  • Journal Title: Fish. Sci Volume: 77 Pages: 983-992
  • NAID: 10029654654
• [Journal Article] Offshore spawning of Conger myriaster in the western North Pacific : evidence for convergent migration strategies of anguilliform eels in the Atlantic and Pacific (2011)
  • Author(s): Miller MJ, Yoshinaga T, Aoyama J, Otake T, Mochioka N, Kurogi H, Tsukamoto K
  • Journal Title: Naturewissenshaften Volume: 98 Pages: 537-543
• [Journal Article] Oceanic spawning ecology of freshwater eels in the western North Pacific (2011)
  • Author(s): Tsukamoto K, Chow S, Otake T, Kurogi H, Mochioka N, Miller MJ, Aoyama J, Kimura S, Watanabe S, Yoshinaga T, Shinoda A, Kuroki M, Oya M, Watanabe T, Hata K, Ijiri S, Kazeto Y, Nomura K, Tanaka H
  • Journal Title: Nature Commutions Volume: 10
• [Journal Article] Evaluation of the larval distribution and migration route of the Japanese eel in the western North Pacific (2011)
  • Author(s): Shinoda A, Aoyama J, Miller MJ, Otake T, Mochioka N, Watanabe S, Minegishi Y, Kuroki M, Yoshinaga T, Yokouchi K, Fukuda N, Sudo R, Hagihara S, Zenimoto K, Suzuki Y, Oya M, Inagaki T, Kimura S, Fukui A, Lee TW, Tsukamoto K
  • Journal Title: Rev. Fish Biol. Fisheries Volume: 21 Pages: 591-611
• [Journal Article] Calorie restriction-induced maternal longevity is transmitted to their daughters in a rotifer (2011)
  • Author(s): Kaneko G, Yoshinaga T, Yanagawa Y, Ozaki Y, Tsukamoto K, Watabe S
  • Journal Title: Func. Ecol Volume: 25 Pages: 209-216
• [Journal Article] シオミズツボワムシの生活史特性と個体群の大変動 (2010)
  • Author(s): 吉永龍起
  • Journal Title: 日本水産学会誌 Volume: 76 Pages: 617-620
  • NAID: 10026550762
• [Presentation] シオミズツボワムシ複合種2株の生活史特性 (2012)
  • Author(s): 島崎, 田中, 小磯, 吉永
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] 酸化耐性を指標としたワムシ個体群の増殖不調の予知 (2012)
  • Author(s): 田中, 小磯, 吉永
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-27
• [Presentation] シオミズツボワムシ複合種2株の生活史特性 (2012)
  • Author(s): 島崎航, 田中千香也, 小磯雅彦, 吉永龍起
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] 酸化耐性を指標としたワムシ個体群の増殖不調の予知 (2012)
  • Author(s): 田中千香也, 小磯雅彦, 吉永龍起
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] 酸化および低水温耐性試験によるワムシ個体群の増殖不良の予測 (2011)
  • Author(s): 吉永, 臼井, 石塚, 田中, 小磯
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-09-29
• [Presentation] 酸化および低水温耐性試験によるワムシ個体群の増殖不良の予測 (2011)
  • Author(s): 吉永龍起, 臼井健洋, 石塚光, 田中千香也, 小磯雅彦
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
• [Presentation] 初期餌料生物シオミズツボワムシの個体数変動に関する研究 (2011)
  • Author(s): 吉永龍起
  • Organizer: 平成22年度日本水産学会東北支部例会
  • Place of Presentation: 宮城